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In vitro antimicrobial activity of a gel containing antimicrobial peptide AMP2041, chlorhexidine digluconate and Tris-EDTA on clinical isolates of Pseudomonas aeruginosa from canine otitis.
Vet Dermatol. 2016 Oct; 27(5):391-e98.VD

Abstract

BACKGROUND

Pseudomonas aeruginosa (PA) may cause suppurative otitis externa with severe inflammation and ulceration in dogs. Multidrug resistance is commonly reported for this organism, creating a difficult therapeutic challenge.

OBJECTIVE

The aim of this study was to evaluate the in vitro antimicrobial activity of a gel containing 0.5 μg/mL of antimicrobial peptide AMP2041, 0.07% chlorhexidine digluconate (CLX), 0.4% Tris and 0.1% EDTA on 30 clinical isolates of PA from canine otitis externa.

MATERIALS AND METHODS

Antimicrobial activity was evaluated through minimal bactericidal concentration (MBC). Standardized bacterial suspensions were incubated with different concentrations of the gel at 37°C for 30 min and plated for colony forming unit (CFU) counts. Time-to-kill kinetics were evaluated with the undiluted product and at MBC for each PA strain at 30 s, 1, 5, 10, 15, 30 min, 24 and 48 h.

RESULTS

The MBC was 1:64 for two of 30 strains, 1:128 for 15 of 30 strains and 1:256 for 13 of 30 strains. The geometric mean was 1:165, equivalent to a concentration of 0.003 μg/mL AMP2041 + 0.0004% CLX + 0.0024%Tris + 0.0006% EDTA. Time-to-kill assays with the undiluted product showed complete bactericidal effect within 30 s for all isolates, whereas at the MBC this effect was reached within 5 min for 20 of 30 isolates and within 30 min for all isolates. Bactericidal activity was maintained after 48 h for all isolates.

CONCLUSION

This gel has shown rapid, complete and long-lasting activity against a panel of 30 PA isolates from cases of canine otitis externa.

Authors+Show Affiliations

Clinica Veterinaria Malpensa, via G. Marconi 27, 21017, Samarate (VA), Italy.Dipartimento di Scienze Medico-Veterinarie, University of Parma, via del Taglio 10, 43126, Parma, Italy.Dipartimento di Scienze Medico-Veterinarie, University of Parma, via del Taglio 10, 43126, Parma, Italy.Dipartimento di Scienze Medico-Veterinarie, University of Parma, via del Taglio 10, 43126, Parma, Italy.Dipartimento di Scienze Medico-Veterinarie, University of Parma, via del Taglio 10, 43126, Parma, Italy.Dipartimento di Scienze Medico-Veterinarie, University of Parma, via del Taglio 10, 43126, Parma, Italy.Dipartimento di Scienze Medico-Veterinarie, University of Parma, via del Taglio 10, 43126, Parma, Italy. clotildesilvia.cabassi@unipr.it.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27545352

Citation

Ghibaudo, Giovanni, et al. "In Vitro Antimicrobial Activity of a Gel Containing Antimicrobial Peptide AMP2041, Chlorhexidine Digluconate and Tris-EDTA On Clinical Isolates of Pseudomonas Aeruginosa From Canine Otitis." Veterinary Dermatology, vol. 27, no. 5, 2016, pp. 391-e98.
Ghibaudo G, Santospirito D, Sala A, et al. In vitro antimicrobial activity of a gel containing antimicrobial peptide AMP2041, chlorhexidine digluconate and Tris-EDTA on clinical isolates of Pseudomonas aeruginosa from canine otitis. Vet Dermatol. 2016;27(5):391-e98.
Ghibaudo, G., Santospirito, D., Sala, A., Flisi, S., Taddei, S., Cavirani, S., & Cabassi, C. S. (2016). In vitro antimicrobial activity of a gel containing antimicrobial peptide AMP2041, chlorhexidine digluconate and Tris-EDTA on clinical isolates of Pseudomonas aeruginosa from canine otitis. Veterinary Dermatology, 27(5), 391-e98. https://doi.org/10.1111/vde.12371
Ghibaudo G, et al. In Vitro Antimicrobial Activity of a Gel Containing Antimicrobial Peptide AMP2041, Chlorhexidine Digluconate and Tris-EDTA On Clinical Isolates of Pseudomonas Aeruginosa From Canine Otitis. Vet Dermatol. 2016;27(5):391-e98. PubMed PMID: 27545352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antimicrobial activity of a gel containing antimicrobial peptide AMP2041, chlorhexidine digluconate and Tris-EDTA on clinical isolates of Pseudomonas aeruginosa from canine otitis. AU - Ghibaudo,Giovanni, AU - Santospirito,Davide, AU - Sala,Andrea, AU - Flisi,Sara, AU - Taddei,Simone, AU - Cavirani,Sandro, AU - Cabassi,Clotilde Silvia, Y1 - 2016/08/21/ PY - 2016/06/29/accepted PY - 2016/8/23/entrez PY - 2016/8/23/pubmed PY - 2017/5/17/medline SP - 391 EP - e98 JF - Veterinary dermatology JO - Vet. Dermatol. VL - 27 IS - 5 N2 - BACKGROUND: Pseudomonas aeruginosa (PA) may cause suppurative otitis externa with severe inflammation and ulceration in dogs. Multidrug resistance is commonly reported for this organism, creating a difficult therapeutic challenge. OBJECTIVE: The aim of this study was to evaluate the in vitro antimicrobial activity of a gel containing 0.5 μg/mL of antimicrobial peptide AMP2041, 0.07% chlorhexidine digluconate (CLX), 0.4% Tris and 0.1% EDTA on 30 clinical isolates of PA from canine otitis externa. MATERIALS AND METHODS: Antimicrobial activity was evaluated through minimal bactericidal concentration (MBC). Standardized bacterial suspensions were incubated with different concentrations of the gel at 37°C for 30 min and plated for colony forming unit (CFU) counts. Time-to-kill kinetics were evaluated with the undiluted product and at MBC for each PA strain at 30 s, 1, 5, 10, 15, 30 min, 24 and 48 h. RESULTS: The MBC was 1:64 for two of 30 strains, 1:128 for 15 of 30 strains and 1:256 for 13 of 30 strains. The geometric mean was 1:165, equivalent to a concentration of 0.003 μg/mL AMP2041 + 0.0004% CLX + 0.0024%Tris + 0.0006% EDTA. Time-to-kill assays with the undiluted product showed complete bactericidal effect within 30 s for all isolates, whereas at the MBC this effect was reached within 5 min for 20 of 30 isolates and within 30 min for all isolates. Bactericidal activity was maintained after 48 h for all isolates. CONCLUSION: This gel has shown rapid, complete and long-lasting activity against a panel of 30 PA isolates from cases of canine otitis externa. SN - 1365-3164 UR - https://www.unboundmedicine.com/medline/citation/27545352/In_vitro_antimicrobial_activity_of_a_gel_containing_antimicrobial_peptide_AMP2041_chlorhexidine_digluconate_and_Tris_EDTA_on_clinical_isolates_of_Pseudomonas_aeruginosa_from_canine_otitis_ L2 - https://doi.org/10.1111/vde.12371 DB - PRIME DP - Unbound Medicine ER -