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Genome-wide identification, phylogeny and expressional profiles of mitogen activated protein kinase kinase kinase (MAPKKK) gene family in bread wheat (Triticum aestivum L.).
BMC Genomics. 2016 08 22; 17:668.BG

Abstract

BACKGROUND

Mitogen-activated protein kinase kinase kinases (MAPKKKs) are the important components of MAPK cascades, which play the crucial role in plant growth and development as well as in response to diverse stresses. Although this family has been systematically studied in many plant species, little is known about MAPKKK genes in wheat (Triticum aestivum L.), especially those involved in the regulatory network of stress processes.

RESULTS

In this study, we identified 155 wheat MAPKKK genes through a genome-wide search method based on the latest available wheat genome information, of which 29 belonged to MEKK, 11 to ZIK and 115 to Raf subfamily, respectively. Then, chromosome localization, gene structure and conserved protein motifs and phylogenetic relationship as well as regulatory network of these TaMAPKKKs were systematically investigated and results supported the prediction. Furthermore, a total of 11 homologous groups between A, B and D sub-genome and 24 duplication pairs among them were detected, which contributed to the expansion of wheat MAPKKK gene family. Finally, the expression profiles of these MAPKKKs during development and under different abiotic stresses were investigated using the RNA-seq data. Additionally, 10 tissue-specific and 4 salt-responsive TaMAPKKK genes were selected to validate their expression level through qRT-PCR analysis.

CONCLUSIONS

This study for the first time reported the genome organization, evolutionary features and expression profiles of the wheat MAPKKK gene family, which laid the foundation for further functional analysis of wheat MAPKKK genes, and contributed to better understanding the roles and regulatory mechanism of MAPKKKs in wheat.

Authors+Show Affiliations

State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, 3 Taicheng Road, Yangling, 712100, Shaanxi, China.State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, 3 Taicheng Road, Yangling, 712100, Shaanxi, China.State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, 3 Taicheng Road, Yangling, 712100, Shaanxi, China.State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, 3 Taicheng Road, Yangling, 712100, Shaanxi, China.State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, 3 Taicheng Road, Yangling, 712100, Shaanxi, China. sweining2002@yahoo.com. Australia-China Joint Research Centre for Abiotic and Biotic Stress Management in Agriculture, Horticulture and Forestry, Yangling, 712100, Shaanxi, China. sweining2002@yahoo.com.State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, 3 Taicheng Road, Yangling, 712100, Shaanxi, China. small@nwsuaf.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27549916

Citation

Wang, Meng, et al. "Genome-wide Identification, Phylogeny and Expressional Profiles of Mitogen Activated Protein Kinase Kinase Kinase (MAPKKK) Gene Family in Bread Wheat (Triticum Aestivum L.)." BMC Genomics, vol. 17, 2016, p. 668.
Wang M, Yue H, Feng K, et al. Genome-wide identification, phylogeny and expressional profiles of mitogen activated protein kinase kinase kinase (MAPKKK) gene family in bread wheat (Triticum aestivum L.). BMC Genomics. 2016;17:668.
Wang, M., Yue, H., Feng, K., Deng, P., Song, W., & Nie, X. (2016). Genome-wide identification, phylogeny and expressional profiles of mitogen activated protein kinase kinase kinase (MAPKKK) gene family in bread wheat (Triticum aestivum L.). BMC Genomics, 17, 668. https://doi.org/10.1186/s12864-016-2993-7
Wang M, et al. Genome-wide Identification, Phylogeny and Expressional Profiles of Mitogen Activated Protein Kinase Kinase Kinase (MAPKKK) Gene Family in Bread Wheat (Triticum Aestivum L.). BMC Genomics. 2016 08 22;17:668. PubMed PMID: 27549916.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide identification, phylogeny and expressional profiles of mitogen activated protein kinase kinase kinase (MAPKKK) gene family in bread wheat (Triticum aestivum L.). AU - Wang,Meng, AU - Yue,Hong, AU - Feng,Kewei, AU - Deng,Pingchuan, AU - Song,Weining, AU - Nie,Xiaojun, Y1 - 2016/08/22/ PY - 2016/03/30/received PY - 2016/08/03/accepted PY - 2016/8/24/entrez PY - 2016/8/24/pubmed PY - 2017/9/12/medline KW - Expression profiles KW - Gene family KW - MAPKKKs KW - Wheat SP - 668 EP - 668 JF - BMC genomics JO - BMC Genomics VL - 17 N2 - BACKGROUND: Mitogen-activated protein kinase kinase kinases (MAPKKKs) are the important components of MAPK cascades, which play the crucial role in plant growth and development as well as in response to diverse stresses. Although this family has been systematically studied in many plant species, little is known about MAPKKK genes in wheat (Triticum aestivum L.), especially those involved in the regulatory network of stress processes. RESULTS: In this study, we identified 155 wheat MAPKKK genes through a genome-wide search method based on the latest available wheat genome information, of which 29 belonged to MEKK, 11 to ZIK and 115 to Raf subfamily, respectively. Then, chromosome localization, gene structure and conserved protein motifs and phylogenetic relationship as well as regulatory network of these TaMAPKKKs were systematically investigated and results supported the prediction. Furthermore, a total of 11 homologous groups between A, B and D sub-genome and 24 duplication pairs among them were detected, which contributed to the expansion of wheat MAPKKK gene family. Finally, the expression profiles of these MAPKKKs during development and under different abiotic stresses were investigated using the RNA-seq data. Additionally, 10 tissue-specific and 4 salt-responsive TaMAPKKK genes were selected to validate their expression level through qRT-PCR analysis. CONCLUSIONS: This study for the first time reported the genome organization, evolutionary features and expression profiles of the wheat MAPKKK gene family, which laid the foundation for further functional analysis of wheat MAPKKK genes, and contributed to better understanding the roles and regulatory mechanism of MAPKKKs in wheat. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/27549916/Genome_wide_identification_phylogeny_and_expressional_profiles_of_mitogen_activated_protein_kinase_kinase_kinase__MAPKKK__gene_family_in_bread_wheat__Triticum_aestivum_L___ L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-2993-7 DB - PRIME DP - Unbound Medicine ER -