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Antioxidant activity and total phenolic and flavonoids content variations of leaves extracts of white Horehound (Marrubium vulgare Linné) from three geographical origins.
Ann Pharm Fr 2016; 74(6):453-462AP

Abstract

OBJECTIFS

To elucidate the effect of the sampling location of Marrubium vulgare L. leaves on phenolic contents and antioxidant proprieties of flavonoids extracts.

MATERIALS AND METHODS

M. vulgare L. leaves were collected from three different geographical locations belonging to northwest Algeria: Tessala (mountain region), M'sila forest (coastal region), and Ain Skhouna (steppe region). The flavonoid extraction was achieved using organic solvents with different polarities (methanol, chloroform, ethyl acetate, and hexane). Folin-Ciocalteu colorimetric method was used for quantification of total phenolic contents, and aluminum chloride assay for quantification of total flavonoid contents. The antioxidant properties of flavonoids extract were studied by free l,l-diphenyl-2-picrylhydrazyl radical-scavenging technique.

RESULTS

Total phenolic and flavonoids concentrations varied respectively between 40.7 and 160mg gallic acid equivalents/g and 27.4 and 66.3mg catechin equivalents/g. The DPPH free radical-scavenging activity shows that the antioxidant activity of the flavonoid extracts varied significantly (P<0.001) depending on the type of the organic solvent used, and the sampling location. The methanol, chloroform and ethyl acetate extracts exhibited the highest percentages of inhibition unlike to the aqueous and hexane extracts. These percentages are ranged from 54.8 to 98.8% at 1000μg/mL. In general, M'sila forest flavonoids extracts showed the highest free radical inhibition capacity; followed by those of Ain Skhouna and Tessala Mountain. The inhibitory concentration 50 (IC50) ranged from 33.7 to 774μg/mL and often exceeded those recorded by phenolic standards (ascorbic acid, gallic acid, caffeic acid, tannic acid and catechin). The phytochemical screening revealed the presence of some flavonoid classes, such as flavans and flavanols.

CONCLUSION

The results suggested a potent antioxidant activity of M. vulgare flavonoids extracts, which may find its application in feature research for the food and the pharmacological industries.

Authors+Show Affiliations

Laboratory of vegetal biodiversity: conservation and valorization, faculty of life and natural sciences, Djillali Liabes university, 22000 Sidi Bel-Abbes, Algeria. Electronic address: bouterfas_karim@yahoo.fr.Laboratory of vegetal biodiversity: conservation and valorization, faculty of life and natural sciences, Djillali Liabes university, 22000 Sidi Bel-Abbes, Algeria.Laboratory of biotoxicology, faculty of life and natural sciences, Djillali Liabes university, 22000 Sidi Bel-Abbes, Algeria.Laboratory of vegetal biodiversity: conservation and valorization, faculty of life and natural sciences, Djillali Liabes university, 22000 Sidi Bel-Abbes, Algeria.Laboratory of vegetal biodiversity: conservation and valorization, faculty of life and natural sciences, Djillali Liabes university, 22000 Sidi Bel-Abbes, Algeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27553439

Citation

Bouterfas, K, et al. "Antioxidant Activity and Total Phenolic and Flavonoids Content Variations of Leaves Extracts of White Horehound (Marrubium Vulgare Linné) From Three Geographical Origins." Annales Pharmaceutiques Francaises, vol. 74, no. 6, 2016, pp. 453-462.
Bouterfas K, Mehdadi Z, Elaoufi MM, et al. Antioxidant activity and total phenolic and flavonoids content variations of leaves extracts of white Horehound (Marrubium vulgare Linné) from three geographical origins. Ann Pharm Fr. 2016;74(6):453-462.
Bouterfas, K., Mehdadi, Z., Elaoufi, M. M., Latreche, A., & Benchiha, W. (2016). Antioxidant activity and total phenolic and flavonoids content variations of leaves extracts of white Horehound (Marrubium vulgare Linné) from three geographical origins. Annales Pharmaceutiques Francaises, 74(6), pp. 453-462. doi:10.1016/j.pharma.2016.07.002.
Bouterfas K, et al. Antioxidant Activity and Total Phenolic and Flavonoids Content Variations of Leaves Extracts of White Horehound (Marrubium Vulgare Linné) From Three Geographical Origins. Ann Pharm Fr. 2016;74(6):453-462. PubMed PMID: 27553439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activity and total phenolic and flavonoids content variations of leaves extracts of white Horehound (Marrubium vulgare Linné) from three geographical origins. AU - Bouterfas,K, AU - Mehdadi,Z, AU - Elaoufi,M M, AU - Latreche,A, AU - Benchiha,W, Y1 - 2016/08/21/ PY - 2016/04/08/received PY - 2016/07/19/revised PY - 2016/07/20/accepted PY - 2016/10/30/pubmed PY - 2017/4/4/medline PY - 2016/8/25/entrez KW - Activité antioxydante KW - Antioxidant activity KW - CI(50) KW - DPPH KW - Extraits flavonoïques KW - Flavonoid extracts KW - Geographical location KW - IC(50) KW - Localité géographique KW - Marrubium vulgare KW - Marrubium vulgare L. SP - 453 EP - 462 JF - Annales pharmaceutiques francaises JO - Ann Pharm Fr VL - 74 IS - 6 N2 - OBJECTIFS: To elucidate the effect of the sampling location of Marrubium vulgare L. leaves on phenolic contents and antioxidant proprieties of flavonoids extracts. MATERIALS AND METHODS: M. vulgare L. leaves were collected from three different geographical locations belonging to northwest Algeria: Tessala (mountain region), M'sila forest (coastal region), and Ain Skhouna (steppe region). The flavonoid extraction was achieved using organic solvents with different polarities (methanol, chloroform, ethyl acetate, and hexane). Folin-Ciocalteu colorimetric method was used for quantification of total phenolic contents, and aluminum chloride assay for quantification of total flavonoid contents. The antioxidant properties of flavonoids extract were studied by free l,l-diphenyl-2-picrylhydrazyl radical-scavenging technique. RESULTS: Total phenolic and flavonoids concentrations varied respectively between 40.7 and 160mg gallic acid equivalents/g and 27.4 and 66.3mg catechin equivalents/g. The DPPH free radical-scavenging activity shows that the antioxidant activity of the flavonoid extracts varied significantly (P<0.001) depending on the type of the organic solvent used, and the sampling location. The methanol, chloroform and ethyl acetate extracts exhibited the highest percentages of inhibition unlike to the aqueous and hexane extracts. These percentages are ranged from 54.8 to 98.8% at 1000μg/mL. In general, M'sila forest flavonoids extracts showed the highest free radical inhibition capacity; followed by those of Ain Skhouna and Tessala Mountain. The inhibitory concentration 50 (IC50) ranged from 33.7 to 774μg/mL and often exceeded those recorded by phenolic standards (ascorbic acid, gallic acid, caffeic acid, tannic acid and catechin). The phytochemical screening revealed the presence of some flavonoid classes, such as flavans and flavanols. CONCLUSION: The results suggested a potent antioxidant activity of M. vulgare flavonoids extracts, which may find its application in feature research for the food and the pharmacological industries. SN - 0003-4509 UR - https://www.unboundmedicine.com/medline/citation/27553439/Antioxidant_activity_and_total_phenolic_and_flavonoids_content_variations_of_leaves_extracts_of_white_Horehound__Marrubium_vulgare_Linné__from_three_geographical_origins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-4509(16)30060-8 DB - PRIME DP - Unbound Medicine ER -