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Heat shock protein 90 is essential for replication of porcine circovirus type 2 in PK-15 cells.
Virus Res. 2016 09 15; 224:29-37.VR

Abstract

Porcine circovirus type 2 (PCV2) is recognized as the causative agent of porcine circovirus-associated disease (PCVAD). However, the mechanism of PCV2 replication has not been understood completely. Heat shock protein 90 (Hsp90) plays an important role in viral genome replication, viral genes expression, and viral particle packaging. In this study, we firstly found that inhibition of Hsp90 by pretreatment of host cells with 17-AAG, a specific inhibitor of Hsp90, or blocking Hsp90α/Hsp90β with siRNA, resulted in significantly reduced viral replication in PK-15 cells. But inhibition of Hsp90 by 17-AAG did not affect PCV2 entry into the host cells. Meanwhile, over-expression of Hsp90α/Hsp90β enhanced PCV2 genome replication and virion production. In addition, Hsp90β was enriched in the nuclear zone in the cells infected with PCV2. But it did not interact with the viral Cap/Rep proteins. It suggested that Hsp90 is required for PCV2 production in PK-15 cells culture. It should be helpful for further evaluating the mechanism of replication and pathogenesis of PCV2 and developing novel antiviral therapies.

Authors+Show Affiliations

Department of Comparative Medicine, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, PR China.Department of Comparative Medicine, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, PR China.Department of Comparative Medicine, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, PR China.Department of Comparative Medicine, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, PR China.Department of Comparative Medicine, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, PR China.Department of Comparative Medicine, Jinling Hospital, 305 East Zhongshan Road, Nanjing 210002, PR China. Electronic address: yunshif@163.com.Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: jiangp@njau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27553861

Citation

Liu, Jie, et al. "Heat Shock Protein 90 Is Essential for Replication of Porcine Circovirus Type 2 in PK-15 Cells." Virus Research, vol. 224, 2016, pp. 29-37.
Liu J, Zhang X, Ma C, et al. Heat shock protein 90 is essential for replication of porcine circovirus type 2 in PK-15 cells. Virus Res. 2016;224:29-37.
Liu, J., Zhang, X., Ma, C., You, J., Dong, M., Yun, S., & Jiang, P. (2016). Heat shock protein 90 is essential for replication of porcine circovirus type 2 in PK-15 cells. Virus Research, 224, 29-37. https://doi.org/10.1016/j.virusres.2016.08.009
Liu J, et al. Heat Shock Protein 90 Is Essential for Replication of Porcine Circovirus Type 2 in PK-15 Cells. Virus Res. 2016 09 15;224:29-37. PubMed PMID: 27553861.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heat shock protein 90 is essential for replication of porcine circovirus type 2 in PK-15 cells. AU - Liu,Jie, AU - Zhang,Xuliang, AU - Ma,Chang, AU - You,Jinwei, AU - Dong,Min, AU - Yun,Shifeng, AU - Jiang,Ping, Y1 - 2016/08/20/ PY - 2016/04/12/received PY - 2016/08/17/revised PY - 2016/08/17/accepted PY - 2016/8/25/entrez PY - 2016/8/25/pubmed PY - 2017/12/2/medline KW - Antiviral KW - Hsp90 KW - Porcine circovirus type 2 KW - Replication SP - 29 EP - 37 JF - Virus research JO - Virus Res. VL - 224 N2 - Porcine circovirus type 2 (PCV2) is recognized as the causative agent of porcine circovirus-associated disease (PCVAD). However, the mechanism of PCV2 replication has not been understood completely. Heat shock protein 90 (Hsp90) plays an important role in viral genome replication, viral genes expression, and viral particle packaging. In this study, we firstly found that inhibition of Hsp90 by pretreatment of host cells with 17-AAG, a specific inhibitor of Hsp90, or blocking Hsp90α/Hsp90β with siRNA, resulted in significantly reduced viral replication in PK-15 cells. But inhibition of Hsp90 by 17-AAG did not affect PCV2 entry into the host cells. Meanwhile, over-expression of Hsp90α/Hsp90β enhanced PCV2 genome replication and virion production. In addition, Hsp90β was enriched in the nuclear zone in the cells infected with PCV2. But it did not interact with the viral Cap/Rep proteins. It suggested that Hsp90 is required for PCV2 production in PK-15 cells culture. It should be helpful for further evaluating the mechanism of replication and pathogenesis of PCV2 and developing novel antiviral therapies. SN - 1872-7492 UR - https://www.unboundmedicine.com/medline/citation/27553861/Heat_shock_protein_90_is_essential_for_replication_of_porcine_circovirus_type_2_in_PK_15_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(16)30243-X DB - PRIME DP - Unbound Medicine ER -