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A sister of PIN1 gene in tomato (Solanum lycopersicum) defines leaf and flower organ initiation patterns by maintaining epidermal auxin flux.
Dev Biol. 2016 11 01; 419(1):85-98.DB

Abstract

The spatiotemporal localization of the plant hormone auxin acts as a positional cue during early leaf and flower organogenesis. One of the main contributors to auxin localization is the auxin efflux carrier PIN-FORMED1 (PIN1). Phylogenetic analysis has revealed that PIN1 genes are split into two sister clades; PIN1 and the relatively uncharacterized Sister-Of-PIN1 (SoPIN1). In this paper we identify entire-2 as a loss-of-function SlSoPIN1a (Solyc10g078370) mutant in Solanum lycopersicum. The entire-2 plants are unable to specify proper leaf initiation leading to a frequent switch from the wild type spiral phyllotactic pattern to distichous and decussate patterns. Leaves in entire-2 are large and less complex and the leaflets display spatial deformities in lamina expansion, vascular development, and margin specification. During sympodial growth in entire-2 the specification of organ position and identity is greatly affected resulting in variable branching patterns on the main sympodial and inflorescence axes. To understand how SlSoPIN1a functions in establishing proper auxin maxima we used the auxin signaling reporter DR5: Venus to visualize differences in auxin localization between entire-2 and wild type. DR5: Venus visualization shows a widening of auxin localization which spreads to subepidermal tissue layers during early leaf and flower organogenesis, showing that SoPIN1 functions to focus auxin signaling to the epidermal layer. The striking spatial deformities observed in entire-2 help provide a mechanistic framework for explaining the function of the SoPIN1 clade in S.lycopersicum.

Authors+Show Affiliations

Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA, USA.Department of Botany and Plant Sciences, University of California at Riverside, Riverside, CA, USA.Donald Danforth Plant Science Center, St. Louis, MO, USA.Department of Plant Biology, University of California at Davis, Davis, CA, USA. Electronic address: nrsinha@ucdavis.edu.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

27554165

Citation

Martinez, Ciera C., et al. "A Sister of PIN1 Gene in Tomato (Solanum Lycopersicum) Defines Leaf and Flower Organ Initiation Patterns By Maintaining Epidermal Auxin Flux." Developmental Biology, vol. 419, no. 1, 2016, pp. 85-98.
Martinez CC, Koenig D, Chitwood DH, et al. A sister of PIN1 gene in tomato (Solanum lycopersicum) defines leaf and flower organ initiation patterns by maintaining epidermal auxin flux. Dev Biol. 2016;419(1):85-98.
Martinez, C. C., Koenig, D., Chitwood, D. H., & Sinha, N. R. (2016). A sister of PIN1 gene in tomato (Solanum lycopersicum) defines leaf and flower organ initiation patterns by maintaining epidermal auxin flux. Developmental Biology, 419(1), 85-98. https://doi.org/10.1016/j.ydbio.2016.08.011
Martinez CC, et al. A Sister of PIN1 Gene in Tomato (Solanum Lycopersicum) Defines Leaf and Flower Organ Initiation Patterns By Maintaining Epidermal Auxin Flux. Dev Biol. 2016 11 1;419(1):85-98. PubMed PMID: 27554165.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A sister of PIN1 gene in tomato (Solanum lycopersicum) defines leaf and flower organ initiation patterns by maintaining epidermal auxin flux. AU - Martinez,Ciera C, AU - Koenig,Daniel, AU - Chitwood,Daniel H, AU - Sinha,Neelima R, Y1 - 2016/08/20/ PY - 2016/05/19/received PY - 2016/07/28/revised PY - 2016/08/12/accepted PY - 2016/8/25/pubmed PY - 2017/8/25/medline PY - 2016/8/25/entrez KW - Auxin signaling KW - Phyllotaxy KW - Sister-of-PIN1 KW - Tomato KW - entire-2 SP - 85 EP - 98 JF - Developmental biology JO - Dev Biol VL - 419 IS - 1 N2 - The spatiotemporal localization of the plant hormone auxin acts as a positional cue during early leaf and flower organogenesis. One of the main contributors to auxin localization is the auxin efflux carrier PIN-FORMED1 (PIN1). Phylogenetic analysis has revealed that PIN1 genes are split into two sister clades; PIN1 and the relatively uncharacterized Sister-Of-PIN1 (SoPIN1). In this paper we identify entire-2 as a loss-of-function SlSoPIN1a (Solyc10g078370) mutant in Solanum lycopersicum. The entire-2 plants are unable to specify proper leaf initiation leading to a frequent switch from the wild type spiral phyllotactic pattern to distichous and decussate patterns. Leaves in entire-2 are large and less complex and the leaflets display spatial deformities in lamina expansion, vascular development, and margin specification. During sympodial growth in entire-2 the specification of organ position and identity is greatly affected resulting in variable branching patterns on the main sympodial and inflorescence axes. To understand how SlSoPIN1a functions in establishing proper auxin maxima we used the auxin signaling reporter DR5: Venus to visualize differences in auxin localization between entire-2 and wild type. DR5: Venus visualization shows a widening of auxin localization which spreads to subepidermal tissue layers during early leaf and flower organogenesis, showing that SoPIN1 functions to focus auxin signaling to the epidermal layer. The striking spatial deformities observed in entire-2 help provide a mechanistic framework for explaining the function of the SoPIN1 clade in S.lycopersicum. SN - 1095-564X UR - https://www.unboundmedicine.com/medline/citation/27554165/A_sister_of_PIN1_gene_in_tomato__Solanum_lycopersicum__defines_leaf_and_flower_organ_initiation_patterns_by_maintaining_epidermal_auxin_flux_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(16)30306-2 DB - PRIME DP - Unbound Medicine ER -