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Protective effect of lavender oil on scopolamine induced cognitive deficits in mice and H2O2 induced cytotoxicity in PC12 cells.
J Ethnopharmacol. 2016 Dec 04; 193:408-415.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Lavender essential oil (LO), an aromatic liquid extracted from Lavandula angustifolia Mill., has been traditionally used in the treatments of many nervous system diseases, and recently LO also reported to be effective for the Alzheimer's disease (AD).

AIM OF THE STUDY

The improvement effect of lavender oil (LO) on the scopolamine-induced cognitive deficits in mice and H2O2 induced cytotoxicity in PC12 cells have been evaluated. The relevant mechanism was also researched from the perspective of antioxidant effect and cholinergic system modulation.

MATERIALS AND METHODS

Cognitive deficits were induced in C57BL/6J mice treated with scopolamine (1mg/kg, i.p.) and were assessed by Morris water maze (MWM) and step-through passive avoidance tests. Then their hippocampus were removed for biochemical assays (acetylcholinesterase (AChE), superoxide dismutase (SOD), glutathione peroxidase (GPX) and malondialdehyde (MDA)). In vitro, the cytotoxicity were induced by 4h exposure to H2O2 in PC12 and evaluated by cell viability (MTT), lactate dehydrogenase (LDH) level, nitric oxide (NO) release, reactive oxygen species (ROS) production and mitochondrial membrane potential (MMP).

RESULTS

The results demonstrated that LO (100mg/kg) could improve the cognitive performance of scopolamine induced mice in behavioral tests. Meanwhile, it significantly decreased the AChE activity, MDA level, and increase SOD and GPX activities of the model. Moreover, LO (12μg/mL) protected PC12 cells from H2O2 induced cytotoxicity by reducing LDH, NO release, intracellular ROS accumulation and MMP loss.

CONCLUSIONS

It was suggested that LO could show neuroprotective effect in AD model in vivo (scopolamine-treated mice) and in vitro (H2O2 induced PC12 cells) via modulating oxidative stress and AChE activity.

Authors+Show Affiliations

Research Center of Pharmacology and Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.Research Center of Pharmacology and Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.Research Center of Pharmacology and Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.Research Center of Pharmacology and Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.Department of Pharmacology and Toxicology Laboratory, Xinjiang Institute of Traditional Uighur Medicine, Urumqi, Xinjiang 830049, China.Department of Pharmacology and Toxicology Laboratory, Xinjiang Institute of Traditional Uighur Medicine, Urumqi, Xinjiang 830049, China.Department of Pharmacology and Toxicology Laboratory, Xinjiang Institute of Traditional Uighur Medicine, Urumqi, Xinjiang 830049, China.Research Center of Pharmacology and Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China. Electronic address: liuxinmin@hotmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27558947

Citation

Xu, Pan, et al. "Protective Effect of Lavender Oil On Scopolamine Induced Cognitive Deficits in Mice and H2O2 Induced Cytotoxicity in PC12 Cells." Journal of Ethnopharmacology, vol. 193, 2016, pp. 408-415.
Xu P, Wang K, Lu C, et al. Protective effect of lavender oil on scopolamine induced cognitive deficits in mice and H2O2 induced cytotoxicity in PC12 cells. J Ethnopharmacol. 2016;193:408-415.
Xu, P., Wang, K., Lu, C., Dong, L., Gao, L., Yan, M., Aibai, S., & Liu, X. (2016). Protective effect of lavender oil on scopolamine induced cognitive deficits in mice and H2O2 induced cytotoxicity in PC12 cells. Journal of Ethnopharmacology, 193, 408-415. https://doi.org/10.1016/j.jep.2016.08.030
Xu P, et al. Protective Effect of Lavender Oil On Scopolamine Induced Cognitive Deficits in Mice and H2O2 Induced Cytotoxicity in PC12 Cells. J Ethnopharmacol. 2016 Dec 4;193:408-415. PubMed PMID: 27558947.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of lavender oil on scopolamine induced cognitive deficits in mice and H2O2 induced cytotoxicity in PC12 cells. AU - Xu,Pan, AU - Wang,Kezhu, AU - Lu,Cong, AU - Dong,Liming, AU - Gao,Li, AU - Yan,Ming, AU - Aibai,Silafu, AU - Liu,Xinmin, Y1 - 2016/08/21/ PY - 2016/03/16/received PY - 2016/08/10/revised PY - 2016/08/20/accepted PY - 2016/8/26/pubmed PY - 2017/4/18/medline PY - 2016/8/26/entrez KW - Lavender oil KW - Morris water maze test KW - Oxidative stress KW - PC12 cells KW - Scopolamine SP - 408 EP - 415 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 193 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Lavender essential oil (LO), an aromatic liquid extracted from Lavandula angustifolia Mill., has been traditionally used in the treatments of many nervous system diseases, and recently LO also reported to be effective for the Alzheimer's disease (AD). AIM OF THE STUDY: The improvement effect of lavender oil (LO) on the scopolamine-induced cognitive deficits in mice and H2O2 induced cytotoxicity in PC12 cells have been evaluated. The relevant mechanism was also researched from the perspective of antioxidant effect and cholinergic system modulation. MATERIALS AND METHODS: Cognitive deficits were induced in C57BL/6J mice treated with scopolamine (1mg/kg, i.p.) and were assessed by Morris water maze (MWM) and step-through passive avoidance tests. Then their hippocampus were removed for biochemical assays (acetylcholinesterase (AChE), superoxide dismutase (SOD), glutathione peroxidase (GPX) and malondialdehyde (MDA)). In vitro, the cytotoxicity were induced by 4h exposure to H2O2 in PC12 and evaluated by cell viability (MTT), lactate dehydrogenase (LDH) level, nitric oxide (NO) release, reactive oxygen species (ROS) production and mitochondrial membrane potential (MMP). RESULTS: The results demonstrated that LO (100mg/kg) could improve the cognitive performance of scopolamine induced mice in behavioral tests. Meanwhile, it significantly decreased the AChE activity, MDA level, and increase SOD and GPX activities of the model. Moreover, LO (12μg/mL) protected PC12 cells from H2O2 induced cytotoxicity by reducing LDH, NO release, intracellular ROS accumulation and MMP loss. CONCLUSIONS: It was suggested that LO could show neuroprotective effect in AD model in vivo (scopolamine-treated mice) and in vitro (H2O2 induced PC12 cells) via modulating oxidative stress and AChE activity. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/27558947/Protective_effect_of_lavender_oil_on_scopolamine_induced_cognitive_deficits_in_mice_and_H2O2_induced_cytotoxicity_in_PC12_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)30556-6 DB - PRIME DP - Unbound Medicine ER -