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Xitong Wan attenuates inflammation development through inhibiting the activation of nuclear factor-κB in rats with adjuvant-induced arthritis.
J Ethnopharmacol. 2016 Dec 04; 193:266-271.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Xitong Wan (XTW), a traditional Chinese herbs formula, has been used to treat "Bi Zheng" in the clinical practice of traditional Chinese medicine (TCM) for hundreds of years. However, no scientific validation is available on the anti-rheumatic effect of XTW.

AIM OF STUDY

This study was carried out to investigate the effects of XTW on joints swelling, joints destruction, production of inflammatory mediators and nuclear factor-κB (NF-κB) activation in rats with adjuvant-induced arthritis (AIA).

MATERIALS AND METHODS

AIA was induced by intradermal injection of Complete Freund's adjuvant in the footpad of Wistar rats. Paw volume was measured every 7 days during XTW treatment. Histological score was calculated by hematoxylin and eosin staining. Osteoclast number in articular tissues was counted by tartrate-resistant acid phosphatase staining. Levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in serum were detected by enzyme-linked immunosorbent assay. Levels of NF-κBp65 and inhibitor of NF-κB (IκB)α in synovium were analyzed by Western blot assay.

RESULTS

Compared with AIA group rats, XTW significantly decreased the paw volume of AIA rats. Meanwhile, XTW significantly reduced the histological score and osteoclast number in articular tissues of AIA rats. In addition, XTW markedly abated the levels of TNF-α, IL-1β and IL-6 in serum, as well as enhanced the level of IκBα in synovium of AIA rats. However, XTW did not show significant effect on the level of p65 in synovium of AIA rats.

CONCLUSIONS

These results suggest that XTW attenuates the inflammation development through inhibiting the NF-κB-mediated proinflammatory cytokines production in AIA rats. Our study provides the scientific evidence of XTW on treatment of rheumatoid arthritis in the clinical practice of TCM.

Authors+Show Affiliations

Department of Combination of Chinese and Western Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, PR China. Electronic address: jiaping008@163.com.College of Environment and Resource, Chongqing Technology and Business University, Chongqing 400067, PR China.Department of Combination of Chinese and Western Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, PR China.Department of Combination of Chinese and Western Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, PR China.Department of Combination of Chinese and Western Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, PR China.Department of Combination of Chinese and Western Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27566198

Citation

Jia, Ping, et al. "Xitong Wan Attenuates Inflammation Development Through Inhibiting the Activation of Nuclear factor-κB in Rats With Adjuvant-induced Arthritis." Journal of Ethnopharmacology, vol. 193, 2016, pp. 266-271.
Jia P, Chen G, Qin WY, et al. Xitong Wan attenuates inflammation development through inhibiting the activation of nuclear factor-κB in rats with adjuvant-induced arthritis. J Ethnopharmacol. 2016;193:266-271.
Jia, P., Chen, G., Qin, W. Y., Zhong, Y., Yang, J., & Rong, X. F. (2016). Xitong Wan attenuates inflammation development through inhibiting the activation of nuclear factor-κB in rats with adjuvant-induced arthritis. Journal of Ethnopharmacology, 193, 266-271. https://doi.org/10.1016/j.jep.2016.08.006
Jia P, et al. Xitong Wan Attenuates Inflammation Development Through Inhibiting the Activation of Nuclear factor-κB in Rats With Adjuvant-induced Arthritis. J Ethnopharmacol. 2016 Dec 4;193:266-271. PubMed PMID: 27566198.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Xitong Wan attenuates inflammation development through inhibiting the activation of nuclear factor-κB in rats with adjuvant-induced arthritis. AU - Jia,Ping, AU - Chen,Gang, AU - Qin,Wen-Yi, AU - Zhong,Yu, AU - Yang,Juan, AU - Rong,Xiao-Feng, Y1 - 2016/08/24/ PY - 2016/03/31/received PY - 2016/07/14/revised PY - 2016/08/03/accepted PY - 2016/8/28/pubmed PY - 2017/6/1/medline PY - 2016/8/28/entrez KW - Adjuvant-induced arthritis KW - Nuclear factor-κB KW - Rheumatoid arthritis KW - Xitong Wan SP - 266 EP - 271 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 193 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Xitong Wan (XTW), a traditional Chinese herbs formula, has been used to treat "Bi Zheng" in the clinical practice of traditional Chinese medicine (TCM) for hundreds of years. However, no scientific validation is available on the anti-rheumatic effect of XTW. AIM OF STUDY: This study was carried out to investigate the effects of XTW on joints swelling, joints destruction, production of inflammatory mediators and nuclear factor-κB (NF-κB) activation in rats with adjuvant-induced arthritis (AIA). MATERIALS AND METHODS: AIA was induced by intradermal injection of Complete Freund's adjuvant in the footpad of Wistar rats. Paw volume was measured every 7 days during XTW treatment. Histological score was calculated by hematoxylin and eosin staining. Osteoclast number in articular tissues was counted by tartrate-resistant acid phosphatase staining. Levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in serum were detected by enzyme-linked immunosorbent assay. Levels of NF-κBp65 and inhibitor of NF-κB (IκB)α in synovium were analyzed by Western blot assay. RESULTS: Compared with AIA group rats, XTW significantly decreased the paw volume of AIA rats. Meanwhile, XTW significantly reduced the histological score and osteoclast number in articular tissues of AIA rats. In addition, XTW markedly abated the levels of TNF-α, IL-1β and IL-6 in serum, as well as enhanced the level of IκBα in synovium of AIA rats. However, XTW did not show significant effect on the level of p65 in synovium of AIA rats. CONCLUSIONS: These results suggest that XTW attenuates the inflammation development through inhibiting the NF-κB-mediated proinflammatory cytokines production in AIA rats. Our study provides the scientific evidence of XTW on treatment of rheumatoid arthritis in the clinical practice of TCM. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/27566198/Xitong_Wan_attenuates_inflammation_development_through_inhibiting_the_activation_of_nuclear_factor_κB_in_rats_with_adjuvant_induced_arthritis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)30526-8 DB - PRIME DP - Unbound Medicine ER -