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The endocannabinoid anandamide impairs in vitro decidualization of human cells.
Reproduction. 2016 10; 152(4):351-61.R

Abstract

Endocannabinoids (eCBs) are endogenous mediators that along with the cannabinoid receptors (CB1 and CB2), a membrane transporter and metabolic enzymes form the endocannabinoid system (ECS). Several eCBs have been discovered with emphasis on anandamide (AEA). They are involved in several biological processes such as energy balance, immune response and reproduction. Decidualization occurs during the secretory phase of human menstrual cycle, which involves proliferation and differentiation of endometrial stromal cells into decidual cells and is crucial for the establishment and progression of pregnancy. In this study, a telomerase-immortalized human endometrial stromal cell line (St-T1b) and non-differentiated primary cultures of human decidual fibroblasts from term placenta were used to characterize the ECS using immunoblotting and qRT-PCR techniques. It was shown that St-T1b cells express CB1, but not CB2, and that both receptors are expressed in HdF cells. Furthermore, the expression of fatty acid amide hydrolase (FAAH), the main degrading enzyme of AEA, increased during stromal cell differentiation. AEA inhibited cell proliferation, through deregulation of cell cycle progression and induced polyploidy. Moreover, through CB1 binding receptor, AEA also impaired cell differentiation. Therefore, AEA is proposed as a modulator of human decidualization. Our findings may provide wider implications, as deregulated levels of AEA, due to Cannabis sativa consumption or altered expression of the metabolic enzymes, may negatively regulate human endometrial stromal cell decidualization with an impact on human (in)fertility.Free Portuguese abstract: A Portuguese translation of this abstract is freely available at http://www.reproduction-online.org/content/152/4/351/suppl/DC1.

Authors+Show Affiliations

UCIBIO@REQUIMTELaboratório de Bioquímica, Departamento Ciências Biológicas, Faculdade de Farmácia da Universidade do Porto, Porto, Portugal.UCIBIO@REQUIMTELaboratório de Bioquímica, Departamento Ciências Biológicas, Faculdade de Farmácia da Universidade do Porto, Porto, Portugal.UCIBIO@REQUIMTELaboratório de Bioquímica, Departamento Ciências Biológicas, Faculdade de Farmácia da Universidade do Porto, Porto, Portugal.UCIBIO@REQUIMTELaboratório de Bioquímica, Departamento Ciências Biológicas, Faculdade de Farmácia da Universidade do Porto, Porto, Portugal.UCIBIO@REQUIMTELaboratório de Bioquímica, Departamento Ciências Biológicas, Faculdade de Farmácia da Universidade do Porto, Porto, Portugal.UCIBIO@REQUIMTELaboratório de Bioquímica, Departamento Ciências Biológicas, Faculdade de Farmácia da Universidade do Porto, Porto, Portugal brunofonseca@ff.up.pt.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27568210

Citation

Almada, M, et al. "The Endocannabinoid Anandamide Impairs in Vitro Decidualization of Human Cells." Reproduction (Cambridge, England), vol. 152, no. 4, 2016, pp. 351-61.
Almada M, Amaral C, Diniz-da-Costa M, et al. The endocannabinoid anandamide impairs in vitro decidualization of human cells. Reproduction. 2016;152(4):351-61.
Almada, M., Amaral, C., Diniz-da-Costa, M., Correia-da-Silva, G., Teixeira, N. A., & Fonseca, B. M. (2016). The endocannabinoid anandamide impairs in vitro decidualization of human cells. Reproduction (Cambridge, England), 152(4), 351-61. https://doi.org/10.1530/REP-16-0364
Almada M, et al. The Endocannabinoid Anandamide Impairs in Vitro Decidualization of Human Cells. Reproduction. 2016;152(4):351-61. PubMed PMID: 27568210.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The endocannabinoid anandamide impairs in vitro decidualization of human cells. AU - Almada,M, AU - Amaral,C, AU - Diniz-da-Costa,M, AU - Correia-da-Silva,G, AU - Teixeira,N A, AU - Fonseca,B M, PY - 2016/04/01/received PY - 2016/08/01/accepted PY - 2016/8/29/entrez PY - 2016/8/29/pubmed PY - 2017/10/31/medline SP - 351 EP - 61 JF - Reproduction (Cambridge, England) JO - Reproduction VL - 152 IS - 4 N2 - Endocannabinoids (eCBs) are endogenous mediators that along with the cannabinoid receptors (CB1 and CB2), a membrane transporter and metabolic enzymes form the endocannabinoid system (ECS). Several eCBs have been discovered with emphasis on anandamide (AEA). They are involved in several biological processes such as energy balance, immune response and reproduction. Decidualization occurs during the secretory phase of human menstrual cycle, which involves proliferation and differentiation of endometrial stromal cells into decidual cells and is crucial for the establishment and progression of pregnancy. In this study, a telomerase-immortalized human endometrial stromal cell line (St-T1b) and non-differentiated primary cultures of human decidual fibroblasts from term placenta were used to characterize the ECS using immunoblotting and qRT-PCR techniques. It was shown that St-T1b cells express CB1, but not CB2, and that both receptors are expressed in HdF cells. Furthermore, the expression of fatty acid amide hydrolase (FAAH), the main degrading enzyme of AEA, increased during stromal cell differentiation. AEA inhibited cell proliferation, through deregulation of cell cycle progression and induced polyploidy. Moreover, through CB1 binding receptor, AEA also impaired cell differentiation. Therefore, AEA is proposed as a modulator of human decidualization. Our findings may provide wider implications, as deregulated levels of AEA, due to Cannabis sativa consumption or altered expression of the metabolic enzymes, may negatively regulate human endometrial stromal cell decidualization with an impact on human (in)fertility.Free Portuguese abstract: A Portuguese translation of this abstract is freely available at http://www.reproduction-online.org/content/152/4/351/suppl/DC1. SN - 1741-7899 UR - https://www.unboundmedicine.com/medline/citation/27568210/The_endocannabinoid_anandamide_impairs_in_vitro_decidualization_of_human_cells_ L2 - https://rep.bioscientifica.com/doi/10.1530/REP-16-0364 DB - PRIME DP - Unbound Medicine ER -