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Induction of oxidative stress, suppression of glucose-induced insulin release, ATP production, glucokinase activity, and histomorphometric changes in pancreatic islets of hypothyroid rat.
Eur J Pharmacol 2016; 791:147-156EJ

Abstract

Thyroid hormones have important role in metabolism and impairment of glucose metabolism and insulin secretion has been shown in hypothyroid rats but the exact mechanisms for this defect are poorly understood. The aim of this study was to investigate the effect of hypothyroidism on oxidative stress parameters, insulin secretory pathway and histomorphometric changes of pancreas. In the isolated islets of the control and methimazole -treated hypothyroid insulin secretion and content, ATP production, Glucokinase, and hexokinase specific activity and kATP and L-type channels sensitivity were assayed. In order to determine oxidative stress parameters, antioxidant enzymes and lipid peroxidation were measured in pancreatic homogenates. Histomorphometric changes and histochemistry of the islet in both groups were compared. Results showed that plasma glucose and insulin concentration and their area under the curve during IPGTT in hypothyroid group were respectively higher and lower than the controls. In the hypothyroid islets, glucose stimulated insulin secretion, ATP production, hexokinase and glucokinase activities were decreased. Hypothyroid induced a significant increased lipid peroxidation, and decreased the antioxidant enzyme activity. Compared with the control group, insulin antibody positivity, the total volume of the pancreas, islets, and the total number as well as the mean volume of the beta cells were also significantly decreased in the hypothyroid group. These findings indicate that oxidative stress produced under hypothyroidism could have a role in progression of pancreatic β-cell dysfunction, reduced beta cell mass and decreased glucokinase activity, impairing glucose tolerance and insulin secretion.

Authors+Show Affiliations

Department of Physiology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Physiology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: karbalai@sums.ac.ir.Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27568837

Citation

Safayee, Sepideh, et al. "Induction of Oxidative Stress, Suppression of Glucose-induced Insulin Release, ATP Production, Glucokinase Activity, and Histomorphometric Changes in Pancreatic Islets of Hypothyroid Rat." European Journal of Pharmacology, vol. 791, 2016, pp. 147-156.
Safayee S, Karbalaei N, Noorafshan A, et al. Induction of oxidative stress, suppression of glucose-induced insulin release, ATP production, glucokinase activity, and histomorphometric changes in pancreatic islets of hypothyroid rat. Eur J Pharmacol. 2016;791:147-156.
Safayee, S., Karbalaei, N., Noorafshan, A., & Nadimi, E. (2016). Induction of oxidative stress, suppression of glucose-induced insulin release, ATP production, glucokinase activity, and histomorphometric changes in pancreatic islets of hypothyroid rat. European Journal of Pharmacology, 791, pp. 147-156. doi:10.1016/j.ejphar.2016.08.024.
Safayee S, et al. Induction of Oxidative Stress, Suppression of Glucose-induced Insulin Release, ATP Production, Glucokinase Activity, and Histomorphometric Changes in Pancreatic Islets of Hypothyroid Rat. Eur J Pharmacol. 2016 Nov 15;791:147-156. PubMed PMID: 27568837.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of oxidative stress, suppression of glucose-induced insulin release, ATP production, glucokinase activity, and histomorphometric changes in pancreatic islets of hypothyroid rat. AU - Safayee,Sepideh, AU - Karbalaei,Narges, AU - Noorafshan,Ali, AU - Nadimi,Elham, Y1 - 2016/08/26/ PY - 2016/04/07/received PY - 2016/08/24/revised PY - 2016/08/25/accepted PY - 2016/10/30/pubmed PY - 2017/3/30/medline PY - 2016/8/30/entrez KW - Beta cell mass KW - Histomorphometry KW - Hypothyroidism KW - Insulin secretion KW - Oxidative stress SP - 147 EP - 156 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 791 N2 - Thyroid hormones have important role in metabolism and impairment of glucose metabolism and insulin secretion has been shown in hypothyroid rats but the exact mechanisms for this defect are poorly understood. The aim of this study was to investigate the effect of hypothyroidism on oxidative stress parameters, insulin secretory pathway and histomorphometric changes of pancreas. In the isolated islets of the control and methimazole -treated hypothyroid insulin secretion and content, ATP production, Glucokinase, and hexokinase specific activity and kATP and L-type channels sensitivity were assayed. In order to determine oxidative stress parameters, antioxidant enzymes and lipid peroxidation were measured in pancreatic homogenates. Histomorphometric changes and histochemistry of the islet in both groups were compared. Results showed that plasma glucose and insulin concentration and their area under the curve during IPGTT in hypothyroid group were respectively higher and lower than the controls. In the hypothyroid islets, glucose stimulated insulin secretion, ATP production, hexokinase and glucokinase activities were decreased. Hypothyroid induced a significant increased lipid peroxidation, and decreased the antioxidant enzyme activity. Compared with the control group, insulin antibody positivity, the total volume of the pancreas, islets, and the total number as well as the mean volume of the beta cells were also significantly decreased in the hypothyroid group. These findings indicate that oxidative stress produced under hypothyroidism could have a role in progression of pancreatic β-cell dysfunction, reduced beta cell mass and decreased glucokinase activity, impairing glucose tolerance and insulin secretion. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/27568837/Induction_of_oxidative_stress_suppression_of_glucose_induced_insulin_release_ATP_production_glucokinase_activity_and_histomorphometric_changes_in_pancreatic_islets_of_hypothyroid_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(16)30543-X DB - PRIME DP - Unbound Medicine ER -