Tags

Type your tag names separated by a space and hit enter

An Outbreak of Infections Caused by a Klebsiella pneumoniae ST11 Clone Coproducing Klebsiella pneumoniae Carbapenemase-2 and RmtB in a Chinese Teaching Hospital.
Chin Med J (Engl). 2016 09 05; 129(17):2033-9.CM

Abstract

BACKGROUND

Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae bacteria, which cause serious disease outbreaks worldwide, was rarely detected in Xiangya Hospital, prior to an outbreak that occurred from August 4, 2014, to March 17, 2015. The aim of this study was to analyze the epidemiology and molecular characteristics of the K. pneumoniae strains isolated during the outbreak.

METHODS

Nonduplicate carbapenem-resistant K. pneumoniae isolates were screened for blaKPC-2and multiple other resistance determinants using polymerase chain reaction. Subsequent studies included pulsed-field gel electrophoresis (PFGE), multilocus sequence typing, analysis of plasmids, and genetic organization of blaKPC-2locus.

RESULTS

Seventeen blaKPC-2-positive K. pneumoniae were identified. A wide range of resistant determinants was detected. Most isolates (88.2%) coharbored blaKPC-2and rmtB in addition to other resistance genes, including blaSHV-1, blaTEM-1, and aac(3)-IIa. The blaKPC-2and rmtB genes were located on the conjugative IncFIB-type plasmid. Genetic organization of blaKPC-2locusin most strains was consistent with that of the plasmid pKP048. Four types (A1, A2, A3, and B) were detected by PFGE, and Type A1, an ST11, was the predominant PFGE type. A novel K. pneumoniae sequence type (ST1883) related to ST11 was discovered.

CONCLUSIONS

These isolates in our study appeared to be clonal and ST11 K. pneumoniae was the predominant clone attributed to the outbreak. Coharbing of blaKPC-2and rmtB, which were located on a transferable plasmid, in clinical K. pneumoniae isolates may lead to the emergence of a new pattern of drug resistance.

Authors+Show Affiliations

Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.Department of Infection Control Center, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27569227

Citation

Li, Jun, et al. "An Outbreak of Infections Caused By a Klebsiella Pneumoniae ST11 Clone Coproducing Klebsiella Pneumoniae Carbapenemase-2 and RmtB in a Chinese Teaching Hospital." Chinese Medical Journal, vol. 129, no. 17, 2016, pp. 2033-9.
Li J, Zou MX, Wang HC, et al. An Outbreak of Infections Caused by a Klebsiella pneumoniae ST11 Clone Coproducing Klebsiella pneumoniae Carbapenemase-2 and RmtB in a Chinese Teaching Hospital. Chin Med J. 2016;129(17):2033-9.
Li, J., Zou, M. X., Wang, H. C., Dou, Q. Y., Hu, Y. M., Yan, Q., & Liu, W. E. (2016). An Outbreak of Infections Caused by a Klebsiella pneumoniae ST11 Clone Coproducing Klebsiella pneumoniae Carbapenemase-2 and RmtB in a Chinese Teaching Hospital. Chinese Medical Journal, 129(17), 2033-9. https://doi.org/10.4103/0366-6999.189049
Li J, et al. An Outbreak of Infections Caused By a Klebsiella Pneumoniae ST11 Clone Coproducing Klebsiella Pneumoniae Carbapenemase-2 and RmtB in a Chinese Teaching Hospital. Chin Med J. 2016 09 5;129(17):2033-9. PubMed PMID: 27569227.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An Outbreak of Infections Caused by a Klebsiella pneumoniae ST11 Clone Coproducing Klebsiella pneumoniae Carbapenemase-2 and RmtB in a Chinese Teaching Hospital. AU - Li,Jun, AU - Zou,Ming-Xiang, AU - Wang,Hai-Chen, AU - Dou,Qing-Ya, AU - Hu,Yong-Mei, AU - Yan,Qun, AU - Liu,Wen-En, PY - 2016/8/30/entrez PY - 2016/8/30/pubmed PY - 2017/3/14/medline SP - 2033 EP - 9 JF - Chinese medical journal JO - Chin. Med. J. VL - 129 IS - 17 N2 - BACKGROUND: Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae bacteria, which cause serious disease outbreaks worldwide, was rarely detected in Xiangya Hospital, prior to an outbreak that occurred from August 4, 2014, to March 17, 2015. The aim of this study was to analyze the epidemiology and molecular characteristics of the K. pneumoniae strains isolated during the outbreak. METHODS: Nonduplicate carbapenem-resistant K. pneumoniae isolates were screened for blaKPC-2and multiple other resistance determinants using polymerase chain reaction. Subsequent studies included pulsed-field gel electrophoresis (PFGE), multilocus sequence typing, analysis of plasmids, and genetic organization of blaKPC-2locus. RESULTS: Seventeen blaKPC-2-positive K. pneumoniae were identified. A wide range of resistant determinants was detected. Most isolates (88.2%) coharbored blaKPC-2and rmtB in addition to other resistance genes, including blaSHV-1, blaTEM-1, and aac(3)-IIa. The blaKPC-2and rmtB genes were located on the conjugative IncFIB-type plasmid. Genetic organization of blaKPC-2locusin most strains was consistent with that of the plasmid pKP048. Four types (A1, A2, A3, and B) were detected by PFGE, and Type A1, an ST11, was the predominant PFGE type. A novel K. pneumoniae sequence type (ST1883) related to ST11 was discovered. CONCLUSIONS: These isolates in our study appeared to be clonal and ST11 K. pneumoniae was the predominant clone attributed to the outbreak. Coharbing of blaKPC-2and rmtB, which were located on a transferable plasmid, in clinical K. pneumoniae isolates may lead to the emergence of a new pattern of drug resistance. SN - 2542-5641 UR - https://www.unboundmedicine.com/medline/citation/27569227/An_Outbreak_of_Infections_Caused_by_a_Klebsiella_pneumoniae_ST11_Clone_Coproducing_Klebsiella_pneumoniae_Carbapenemase_2_and_RmtB_in_a_Chinese_Teaching_Hospital_ L2 - http://dx.doi.org/10.4103/0366-6999.189049 DB - PRIME DP - Unbound Medicine ER -