Tags

Type your tag names separated by a space and hit enter

Cyclophilin B protects SH-SY5Y human neuroblastoma cells against MPP(+)-induced neurotoxicity via JNK pathway.
Biochem Biophys Res Commun. 2016 09 23; 478(3):1396-402.BB

Abstract

Parkinson's disease (PD) is the second most common neurodegenerative disorder of aging. PD involves a progressive loss of dopaminergic neurons in the substantia nigra pars compacta. 1-Methyl-4-phenyl-1, 2, 3, 6-tetrahydropyidine (MPTP) and its toxic metabolite 1-methyl-4-phenylpyridinium ion (MPP+) inhibit the complex I of the mitochondrial electron transport chain, and have been widely used to construct PD models. Cyclophilin B (CypB) is an endoplasmic reticulum protein that binds to cyclosporine A as a cyclophilin family member. CypB has peptidyl-prolyl cis-trans isomerase (PPIase) activity. We investigated the protective effects of overexpressed CypB on MPP+-induced neurocytotoxicity in SH-SY5Y human neuroblastoma cells. Overexpressed CypB decreased MPP(+)-induced oxidative stress through the modulation of antioxidant enzymes including manganese superoxide dismutase and catalase, and prevented neurocytotoxicity via mitogen-activated protein kinase, especially the c-Jun N-terminal kinase pathway. In addition, CypB inhibited the activation of MPP(+)-induced the pro-apoptotic molecules poly (ADP-ribose) polymerase, Bax, and Bcl-2, and attenuated MPP(+)-induced mitochondrial dysfunction. The data suggest that overexpressed CypB protects neuronal cells from MPP+-induced dopaminergic neuronal cell death.

Authors+Show Affiliations

Department of Biomedical Science, Graduate School, Kyung Hee University, Republic of Korea.Department of Biomedical Science, Graduate School, Kyung Hee University, Republic of Korea.Department of Biomedical Science, Graduate School, Kyung Hee University, Republic of Korea.Department of Biomedical Science, Graduate School, Kyung Hee University, Republic of Korea.Department of Biomedical Science, Graduate School, Kyung Hee University, Republic of Korea.Department of Biochemistry and Molecular Biology, Medicine Research Center for Bioreaction of Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, Republic of Korea.Department of Biochemistry and Molecular Biology, Medicine Research Center for Bioreaction of Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, Republic of Korea.Department of Biochemistry and Molecular Biology, Medicine Research Center for Bioreaction of Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, Republic of Korea.Department of Biochemistry and Molecular Biology, Medicine Research Center for Bioreaction of Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, Republic of Korea.Department of Biochemistry and Molecular Biology, Medicine Research Center for Bioreaction of Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, Republic of Korea. Electronic address: wchoe@khu.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27569281

Citation

Oh, Yoojung, et al. "Cyclophilin B Protects SH-SY5Y Human Neuroblastoma Cells Against MPP(+)-induced Neurotoxicity Via JNK Pathway." Biochemical and Biophysical Research Communications, vol. 478, no. 3, 2016, pp. 1396-402.
Oh Y, Jeong K, Kim K, et al. Cyclophilin B protects SH-SY5Y human neuroblastoma cells against MPP(+)-induced neurotoxicity via JNK pathway. Biochem Biophys Res Commun. 2016;478(3):1396-402.
Oh, Y., Jeong, K., Kim, K., Lee, Y. S., Jeong, S., Kim, S. S., Yoon, K. S., Ha, J., Kang, I., & Choe, W. (2016). Cyclophilin B protects SH-SY5Y human neuroblastoma cells against MPP(+)-induced neurotoxicity via JNK pathway. Biochemical and Biophysical Research Communications, 478(3), 1396-402. https://doi.org/10.1016/j.bbrc.2016.08.135
Oh Y, et al. Cyclophilin B Protects SH-SY5Y Human Neuroblastoma Cells Against MPP(+)-induced Neurotoxicity Via JNK Pathway. Biochem Biophys Res Commun. 2016 09 23;478(3):1396-402. PubMed PMID: 27569281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cyclophilin B protects SH-SY5Y human neuroblastoma cells against MPP(+)-induced neurotoxicity via JNK pathway. AU - Oh,Yoojung, AU - Jeong,Kwon, AU - Kim,Kiyoon, AU - Lee,Young-Seok, AU - Jeong,Suyun, AU - Kim,Sung Soo, AU - Yoon,Kyung-Sik, AU - Ha,Joohun, AU - Kang,Insug, AU - Choe,Wonchae, Y1 - 2016/08/25/ PY - 2016/08/18/received PY - 2016/08/24/accepted PY - 2016/8/30/entrez PY - 2016/8/30/pubmed PY - 2017/5/30/medline KW - Apoptosis KW - Cyclophilin B KW - JNK KW - MPP+ KW - Parkinson's disease SP - 1396 EP - 402 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 478 IS - 3 N2 - Parkinson's disease (PD) is the second most common neurodegenerative disorder of aging. PD involves a progressive loss of dopaminergic neurons in the substantia nigra pars compacta. 1-Methyl-4-phenyl-1, 2, 3, 6-tetrahydropyidine (MPTP) and its toxic metabolite 1-methyl-4-phenylpyridinium ion (MPP+) inhibit the complex I of the mitochondrial electron transport chain, and have been widely used to construct PD models. Cyclophilin B (CypB) is an endoplasmic reticulum protein that binds to cyclosporine A as a cyclophilin family member. CypB has peptidyl-prolyl cis-trans isomerase (PPIase) activity. We investigated the protective effects of overexpressed CypB on MPP+-induced neurocytotoxicity in SH-SY5Y human neuroblastoma cells. Overexpressed CypB decreased MPP(+)-induced oxidative stress through the modulation of antioxidant enzymes including manganese superoxide dismutase and catalase, and prevented neurocytotoxicity via mitogen-activated protein kinase, especially the c-Jun N-terminal kinase pathway. In addition, CypB inhibited the activation of MPP(+)-induced the pro-apoptotic molecules poly (ADP-ribose) polymerase, Bax, and Bcl-2, and attenuated MPP(+)-induced mitochondrial dysfunction. The data suggest that overexpressed CypB protects neuronal cells from MPP+-induced dopaminergic neuronal cell death. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/27569281/Cyclophilin_B_protects_SH_SY5Y_human_neuroblastoma_cells_against_MPP_+__induced_neurotoxicity_via_JNK_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(16)31399-7 DB - PRIME DP - Unbound Medicine ER -