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Effect of hydroxyurea on the rheological properties of sickle erythrocytes in vivo.

Abstract

We have monitored the rheological effects of hydroxyurea (HU) on erythrocytes obtained from two patients with severe sickle cell anemia who were enrolled in a therapeutic trial of this drug. Erythrocyte membrane stability and whole cell and membrane deformability of red cells from treated and untreated patients and normal controls were determined in room air using an ektacytometer--a laser viscodiffractometer. The percentage of dense cells was quantitated by centrifugation on a discontinuous Stractan density gradient. F reticulocytes (FR), absolute F reticulocytes (AFR), and F cells (FC) were determined by single-cell radial immunolgic assays. After 1 year of treatment with HU, there was a significant increase in the level of hemoglobin (Hb) F, FR, AFR, and FC. The degree of anemia remained the same, but there was significant increase in the mean cell volume (MCV) and a significant decrease in the mean corpuscular Hb concentration (MCHC). Whole cell deformability improved by twofold, but membrane stability remained within normal limits. The hydration status of sickle erythrocytes improved as was indicated by a change toward normal in gradient osmotic ektacytometry, an increase in RBC K+ content, a decrease in percent of dense cells, and a decrease in the MCHC. The data indicate that, in addition to its effect on the production of Hb, F, HU has a salutary effect on whole cell deformability and on the hydration status of sickle erythrocytes. Determination of the rheological properties of erythrocytes may be of value in monitoring the response to HU.

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  • Authors+Show Affiliations

    ,

    Cardeza Foundation for Hematologic Research, Philadelphia, PA 19107.

    ,

    Source

    American journal of hematology 32:2 1989 Oct pg 104-11

    MeSH

    Anemia, Sickle Cell
    Erythrocyte Deformability
    Erythrocyte Indices
    Erythrocyte Membrane
    Genotype
    Globins
    Humans
    Hydroxyurea

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't
    Research Support, U.S. Gov't, P.H.S.

    Language

    eng

    PubMed ID

    2757007

    Citation

    Ballas, S K., et al. "Effect of Hydroxyurea On the Rheological Properties of Sickle Erythrocytes in Vivo." American Journal of Hematology, vol. 32, no. 2, 1989, pp. 104-11.
    Ballas SK, Dover GJ, Charache S. Effect of hydroxyurea on the rheological properties of sickle erythrocytes in vivo. Am J Hematol. 1989;32(2):104-11.
    Ballas, S. K., Dover, G. J., & Charache, S. (1989). Effect of hydroxyurea on the rheological properties of sickle erythrocytes in vivo. American Journal of Hematology, 32(2), pp. 104-11.
    Ballas SK, Dover GJ, Charache S. Effect of Hydroxyurea On the Rheological Properties of Sickle Erythrocytes in Vivo. Am J Hematol. 1989;32(2):104-11. PubMed PMID: 2757007.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effect of hydroxyurea on the rheological properties of sickle erythrocytes in vivo. AU - Ballas,S K, AU - Dover,G J, AU - Charache,S, PY - 1989/10/1/pubmed PY - 1989/10/1/medline PY - 1989/10/1/entrez SP - 104 EP - 11 JF - American journal of hematology JO - Am. J. Hematol. VL - 32 IS - 2 N2 - We have monitored the rheological effects of hydroxyurea (HU) on erythrocytes obtained from two patients with severe sickle cell anemia who were enrolled in a therapeutic trial of this drug. Erythrocyte membrane stability and whole cell and membrane deformability of red cells from treated and untreated patients and normal controls were determined in room air using an ektacytometer--a laser viscodiffractometer. The percentage of dense cells was quantitated by centrifugation on a discontinuous Stractan density gradient. F reticulocytes (FR), absolute F reticulocytes (AFR), and F cells (FC) were determined by single-cell radial immunolgic assays. After 1 year of treatment with HU, there was a significant increase in the level of hemoglobin (Hb) F, FR, AFR, and FC. The degree of anemia remained the same, but there was significant increase in the mean cell volume (MCV) and a significant decrease in the mean corpuscular Hb concentration (MCHC). Whole cell deformability improved by twofold, but membrane stability remained within normal limits. The hydration status of sickle erythrocytes improved as was indicated by a change toward normal in gradient osmotic ektacytometry, an increase in RBC K+ content, a decrease in percent of dense cells, and a decrease in the MCHC. The data indicate that, in addition to its effect on the production of Hb, F, HU has a salutary effect on whole cell deformability and on the hydration status of sickle erythrocytes. Determination of the rheological properties of erythrocytes may be of value in monitoring the response to HU. SN - 0361-8609 UR - https://www.unboundmedicine.com/medline/citation/2757007/Effect_of_hydroxyurea_on_the_rheological_properties_of_sickle_erythrocytes_in_vivo_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0361-8609&date=1989&volume=32&issue=2&spage=104 DB - PRIME DP - Unbound Medicine ER -