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Comparison of two chemiluminescent immunoassays in the detection of measles IgM antibodies.
J Virol Methods. 2016 11; 237:38-39.JV

Abstract

Serological confirmation of measles is achieved by detecting the specific immunoglobulin M (IgM), and it is important to evaluate new commercial inmunoassays in order to ensure the quality of results. The objective of this study was to compare the performance of a novel automated chemiluminescent immunoassay (CLIA), Virclia IgM measles (Vircell, Spain), with that of the widely used Liaison measles IgM assay (DiaSorin, Italy). A panel of 86 sera from laboratory-confirmed cases was used for the sensitivity calculation, and 59 sera from healthy individuals and those with other viral infections were used for the specificity calculation. Sensitivity values were 96.5% for Virclia and 97.6% for Liaison; specificity values were 93.2% for Virclia and 96.6% for Liaison; neither difference was statistically significant VirClia IgM measles is a good alternative to other immunoassays for the serological confirmation of measles.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Gómez-Camarasa C
Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: gomezcamarasa@gmail.com.
Lara-Oya A
Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: ana.lara.sspa@juntadeandalucia.es.
Cobo F
Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: fernando.cobo.sspa@juntadeandalucia.es.
Sampedro-Martínez A
Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: antonioj.sampedro.sspa@juntadeandalucia.es.
Rodríguez-Granger J
Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: javierm.rodriguez.sspa@juntadeandalucia.es.
Gutierrez-Fernández J
Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: josegf@ugr.es.
Navarro-Marí JM
Microbiology Department; University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014, Granada, Spain. Electronic address: josem.navarro.sspa@juntadeandalucia.es.

MeSH

Antibodies, ViralHumansImmunoassayImmunoenzyme TechniquesImmunoglobulin GImmunoglobulin MItalyLuminescent MeasurementsMeaslesMeasles virusReagent Kits, DiagnosticSensitivity and SpecificitySerologic TestsSpain

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

27575683

Citation

Gómez-Camarasa, Cristina, et al. "Comparison of Two Chemiluminescent Immunoassays in the Detection of Measles IgM Antibodies." Journal of Virological Methods, vol. 237, 2016, pp. 38-39.
Gómez-Camarasa C, Lara-Oya A, Cobo F, et al. Comparison of two chemiluminescent immunoassays in the detection of measles IgM antibodies. J Virol Methods. 2016;237:38-39.
Gómez-Camarasa, C., Lara-Oya, A., Cobo, F., Sampedro-Martínez, A., Rodríguez-Granger, J., Gutierrez-Fernández, J., & Navarro-Marí, J. M. (2016). Comparison of two chemiluminescent immunoassays in the detection of measles IgM antibodies. Journal of Virological Methods, 237, 38-39. https://doi.org/10.1016/j.jviromet.2016.08.018
Gómez-Camarasa C, et al. Comparison of Two Chemiluminescent Immunoassays in the Detection of Measles IgM Antibodies. J Virol Methods. 2016;237:38-39. PubMed PMID: 27575683.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of two chemiluminescent immunoassays in the detection of measles IgM antibodies. AU - Gómez-Camarasa,Cristina, AU - Lara-Oya,Ana, AU - Cobo,Fernando, AU - Sampedro-Martínez,Antonio, AU - Rodríguez-Granger,Javier, AU - Gutierrez-Fernández,José, AU - Navarro-Marí,José María, Y1 - 2016/08/26/ PY - 2016/05/20/received PY - 2016/08/24/revised PY - 2016/08/25/accepted PY - 2016/8/31/pubmed PY - 2017/8/2/medline PY - 2016/8/31/entrez KW - Chemiluminescent immunoassay KW - Measles KW - Serology SP - 38 EP - 39 JF - Journal of virological methods JO - J Virol Methods VL - 237 N2 - Serological confirmation of measles is achieved by detecting the specific immunoglobulin M (IgM), and it is important to evaluate new commercial inmunoassays in order to ensure the quality of results. The objective of this study was to compare the performance of a novel automated chemiluminescent immunoassay (CLIA), Virclia IgM measles (Vircell, Spain), with that of the widely used Liaison measles IgM assay (DiaSorin, Italy). A panel of 86 sera from laboratory-confirmed cases was used for the sensitivity calculation, and 59 sera from healthy individuals and those with other viral infections were used for the specificity calculation. Sensitivity values were 96.5% for Virclia and 97.6% for Liaison; specificity values were 93.2% for Virclia and 96.6% for Liaison; neither difference was statistically significant VirClia IgM measles is a good alternative to other immunoassays for the serological confirmation of measles. SN - 1879-0984 UR - https://www.unboundmedicine.com/medline/citation/27575683/Comparison_of_two_chemiluminescent_immunoassays_in_the_detection_of_measles_IgM_antibodies_ DB - PRIME DP - Unbound Medicine ER -