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Enhanced cellulase production from Trichoderma reesei Rut-C30 by engineering with an artificial zinc finger protein library.
Biotechnol J. 2016 Oct; 11(10):1282-1290.BJ

Abstract

Trichoderma reesei Rut-C30 is a well-known cellulase producer, and improvement of its cellulase production is of great interest. An artificial zinc finger protein (AZFP) library is constructed for expression in T. reesei Rut-C30, and a mutant strain T. reesei U3 is selected based on its enhanced cellulase production. The U3 mutant shows a 55% rise in filter paper activity and 8.1-fold increased β-glucosidase activity, when compared to the native strain T. reesei Rut-C30. It is demonstrated that enhanced β-glucosidase activity was due to elevated transcription level of β-glucosidase gene in the U3 mutant. Moreover, significant elevation in transcription levels of several putative Azfp-U3 target genes is detected in the U3 mutant, including genes encoding hypothetical transcription factors and a putative glycoside hydrolase. Furthermore, U3 cellulase shows 115% higher glucose yield from pretreated corn stover, when compared to the cellulase of T. reesei Rut-C30. These results demonstrate that AZFP can be used to improve cellulase production in T. reesei Rut-C30. Our current work offers the establishment of an alternative strategy to develop fungal cell factories for improved production of high value industrial products.

Authors+Show Affiliations

School of Life Science and Biotechnology, Dalian University of Technology, Dalian, China.School of Life Science and Biotechnology, Dalian University of Technology, Dalian, China. State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, China. xqzhao@sjtu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27578229

Citation

Zhang, Fei, et al. "Enhanced Cellulase Production From Trichoderma Reesei Rut-C30 By Engineering With an Artificial Zinc Finger Protein Library." Biotechnology Journal, vol. 11, no. 10, 2016, pp. 1282-1290.
Zhang F, Bai F, Zhao X. Enhanced cellulase production from Trichoderma reesei Rut-C30 by engineering with an artificial zinc finger protein library. Biotechnol J. 2016;11(10):1282-1290.
Zhang, F., Bai, F., & Zhao, X. (2016). Enhanced cellulase production from Trichoderma reesei Rut-C30 by engineering with an artificial zinc finger protein library. Biotechnology Journal, 11(10), 1282-1290. https://doi.org/10.1002/biot.201600227
Zhang F, Bai F, Zhao X. Enhanced Cellulase Production From Trichoderma Reesei Rut-C30 By Engineering With an Artificial Zinc Finger Protein Library. Biotechnol J. 2016;11(10):1282-1290. PubMed PMID: 27578229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced cellulase production from Trichoderma reesei Rut-C30 by engineering with an artificial zinc finger protein library. AU - Zhang,Fei, AU - Bai,Fengwu, AU - Zhao,Xinqing, Y1 - 2016/09/14/ PY - 2016/04/10/received PY - 2016/08/25/revised PY - 2016/08/29/accepted PY - 2016/9/1/pubmed PY - 2017/1/20/medline PY - 2016/9/1/entrez KW - Artificial transcription factor (ATF) KW - Artificial zinc finger protein (AZFP) KW - Cellulase KW - Trichoderma reesei Rut-C30 KW - β-glucosidase SP - 1282 EP - 1290 JF - Biotechnology journal JO - Biotechnol J VL - 11 IS - 10 N2 - Trichoderma reesei Rut-C30 is a well-known cellulase producer, and improvement of its cellulase production is of great interest. An artificial zinc finger protein (AZFP) library is constructed for expression in T. reesei Rut-C30, and a mutant strain T. reesei U3 is selected based on its enhanced cellulase production. The U3 mutant shows a 55% rise in filter paper activity and 8.1-fold increased β-glucosidase activity, when compared to the native strain T. reesei Rut-C30. It is demonstrated that enhanced β-glucosidase activity was due to elevated transcription level of β-glucosidase gene in the U3 mutant. Moreover, significant elevation in transcription levels of several putative Azfp-U3 target genes is detected in the U3 mutant, including genes encoding hypothetical transcription factors and a putative glycoside hydrolase. Furthermore, U3 cellulase shows 115% higher glucose yield from pretreated corn stover, when compared to the cellulase of T. reesei Rut-C30. These results demonstrate that AZFP can be used to improve cellulase production in T. reesei Rut-C30. Our current work offers the establishment of an alternative strategy to develop fungal cell factories for improved production of high value industrial products. SN - 1860-7314 UR - https://www.unboundmedicine.com/medline/citation/27578229/Enhanced_cellulase_production_from_Trichoderma_reesei_Rut_C30_by_engineering_with_an_artificial_zinc_finger_protein_library_ L2 - https://doi.org/10.1002/biot.201600227 DB - PRIME DP - Unbound Medicine ER -