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Comparison of faecal microbiota in Blastocystis-positive and Blastocystis-negative irritable bowel syndrome patients.

Abstract

BACKGROUND

We investigated whether the carriage of Blastocystis in IBS patients was associated with differences in the faecal microbiota. Forty patients with diarrhoea-predominant IBS (26 Blastocystis-positive and 14 Blastocystis-negative) and 57 healthy controls (HC) (42 Blastocystis-positive and 15 Blastocystis-negative) submitted faecal samples for metataxonomic analysis of the 16S ribosomal RNA gene. Differences in the relative abundance of bacteria in these IBS and HC groups were evaluated from phylum to genus level.

RESULTS

Significant changes were observed in two dominant phyla in IBS patients, regardless of Blastocystis infection status, namely a rise in Firmicutes and a statistically significant reduction in relative abundance of Bacteroidetes (with a threefold increase in the Firmicutes to Bacteoridetes ratio). Significant differences at genus level in IBS subjects compared to HC were also observed for many bacterial species. However, further clinical subgroup analysis of Blastocystis-positive and Blastocystis-negative subjects, regardless of symptoms, showed no significant differences at the phylum or genus level in IBS-P compared to IBS-N.

CONCLUSIONS

Significant differences in the faecal microbiota between diarrhoea-predominant IBS patients and healthy controls were confirmed, but the carriage of Blastocystis did not significantly alter the faecal microbiota. If Blastocystis-positive patients represent a separate clinical subtype of IBS, this group is not identified by changes in the microbiota.

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  • Authors+Show Affiliations

    ,

    School of Veterinary Science, The University of Queensland, Gatton Campus, Brisbane, Queensland, 4343, Australia. robyn@tgclinic.com.au. Toowoomba Gastroenterology Clinic, Suite 105 Medici Medical Centre, 15 Scott St, Toowoomba, QLD, 4350, Australia. robyn@tgclinic.com.au.

    ,

    Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, Melbourne, Victoria, 3052, Australia.

    ,

    School of Pathology and Laboratory Medicine, University of Western Australia, Crawley, Western Australia, 6009, Australia.

    ,

    Rural Clinical School, School of Medicine, The University of Queensland, Toowoomba, 4350, Australia.

    Australian Infectious Diseases Research Centre, The University of Queensland, St. Lucia, Queensland, 4072, Australia.

    Source

    Microbiome 4:1 2016 08 31 pg 47

    MeSH

    Adult
    Bacterial Load
    Bacteroidetes
    Base Sequence
    Blastocystis
    Blastocystis Infections
    Feces
    Female
    Firmicutes
    Humans
    Irritable Bowel Syndrome
    Male
    Microbiota
    Middle Aged
    RNA, Ribosomal, 16S
    Sequence Analysis, DNA

    Pub Type(s)

    Comparative Study
    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    27580855

    Citation

    Nagel, Robyn, et al. "Comparison of Faecal Microbiota in Blastocystis-positive and Blastocystis-negative Irritable Bowel Syndrome Patients." Microbiome, vol. 4, no. 1, 2016, p. 47.
    Nagel R, Traub RJ, Allcock RJ, et al. Comparison of faecal microbiota in Blastocystis-positive and Blastocystis-negative irritable bowel syndrome patients. Microbiome. 2016;4(1):47.
    Nagel, R., Traub, R. J., Allcock, R. J., Kwan, M. M., & Bielefeldt-Ohmann, H. (2016). Comparison of faecal microbiota in Blastocystis-positive and Blastocystis-negative irritable bowel syndrome patients. Microbiome, 4(1), p. 47. doi:10.1186/s40168-016-0191-0.
    Nagel R, et al. Comparison of Faecal Microbiota in Blastocystis-positive and Blastocystis-negative Irritable Bowel Syndrome Patients. Microbiome. 2016 08 31;4(1):47. PubMed PMID: 27580855.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Comparison of faecal microbiota in Blastocystis-positive and Blastocystis-negative irritable bowel syndrome patients. AU - Nagel,Robyn, AU - Traub,Rebecca J, AU - Allcock,Richard J N, AU - Kwan,Marcella M S, AU - Bielefeldt-Ohmann,Helle, Y1 - 2016/08/31/ PY - 2015/10/04/received PY - 2016/08/09/accepted PY - 2016/9/2/entrez PY - 2016/9/2/pubmed PY - 2017/7/7/medline KW - Blastocystis KW - Faecal microbiota KW - Irritable bowel syndrome SP - 47 EP - 47 JF - Microbiome JO - Microbiome VL - 4 IS - 1 N2 - BACKGROUND: We investigated whether the carriage of Blastocystis in IBS patients was associated with differences in the faecal microbiota. Forty patients with diarrhoea-predominant IBS (26 Blastocystis-positive and 14 Blastocystis-negative) and 57 healthy controls (HC) (42 Blastocystis-positive and 15 Blastocystis-negative) submitted faecal samples for metataxonomic analysis of the 16S ribosomal RNA gene. Differences in the relative abundance of bacteria in these IBS and HC groups were evaluated from phylum to genus level. RESULTS: Significant changes were observed in two dominant phyla in IBS patients, regardless of Blastocystis infection status, namely a rise in Firmicutes and a statistically significant reduction in relative abundance of Bacteroidetes (with a threefold increase in the Firmicutes to Bacteoridetes ratio). Significant differences at genus level in IBS subjects compared to HC were also observed for many bacterial species. However, further clinical subgroup analysis of Blastocystis-positive and Blastocystis-negative subjects, regardless of symptoms, showed no significant differences at the phylum or genus level in IBS-P compared to IBS-N. CONCLUSIONS: Significant differences in the faecal microbiota between diarrhoea-predominant IBS patients and healthy controls were confirmed, but the carriage of Blastocystis did not significantly alter the faecal microbiota. If Blastocystis-positive patients represent a separate clinical subtype of IBS, this group is not identified by changes in the microbiota. SN - 2049-2618 UR - https://www.unboundmedicine.com/medline/citation/27580855/Comparison_of_faecal_microbiota_in_Blastocystis_positive_and_Blastocystis_negative_irritable_bowel_syndrome_patients_ L2 - https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-016-0191-0 DB - PRIME DP - Unbound Medicine ER -