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Transcriptional Analysis of The Adaptive Digestive System of The Migratory Locust in Response to Plant Defensive Protease Inhibitors.
Sci Rep. 2016 09 01; 6:32460.SR

Abstract

Herbivorous insects evolved adaptive mechanisms to compensate for the presence of plant defensive protease inhibitors (PI) in their food. The underlying regulatory mechanisms of these compensatory responses remain largely elusive. In the current study, we investigated the initiation of this adaptive response in the migratory locust, Locusta migratoria, via microarray analysis of gut tissues. Four hours after dietary uptake of PIs, 114 and 150 transcripts were respectively found up- or downregulated. The results suggest a quick trade-off between compensating for potential loss of digestive activity on the one hand, and stress tolerance, defense, and structural integrity of the gut on the other hand. We additionally addressed the role of a group of related upregulated hexamerin-like proteins in the PI-induced response. Simultaneous knockdown of corresponding transcripts by means of RNA interference resulted in a reduced capacity of the locust nymphs to cope with the effects of PI. Moreover, since insect hexamerins have been shown to bind Juvenile Hormone (JH), we also investigated the effect of JH on the proteolytic digestion in L. migratoria. Our results indicate that JH has a stimulatory effect on the expression of three homologous chymotrypsin genes, while knocking down the JH receptor (methoprene tolerant) led to opposite effects.

Authors+Show Affiliations

Department of Animal Physiology and Neurobiology, Zoological Institute KU Leuven, Naamsestraat 59, B-3000 Leuven, Belgium.Department of Animal Physiology and Neurobiology, Zoological Institute KU Leuven, Naamsestraat 59, B-3000 Leuven, Belgium.Department of Animal Physiology and Neurobiology, Zoological Institute KU Leuven, Naamsestraat 59, B-3000 Leuven, Belgium.Systemic Physiological and Ecotoxicological Research (SPHERE), Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerpen, Belgium. Zebrafishlab, Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium.Department of Animal Physiology and Neurobiology, Zoological Institute KU Leuven, Naamsestraat 59, B-3000 Leuven, Belgium.Systemic Physiological and Ecotoxicological Research (SPHERE), Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerpen, Belgium. Zebrafishlab, Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium.Department of Animal Physiology and Neurobiology, Zoological Institute KU Leuven, Naamsestraat 59, B-3000 Leuven, Belgium.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27581362

Citation

Spit, Jornt, et al. "Transcriptional Analysis of the Adaptive Digestive System of the Migratory Locust in Response to Plant Defensive Protease Inhibitors." Scientific Reports, vol. 6, 2016, p. 32460.
Spit J, Holtof M, Badisco L, et al. Transcriptional Analysis of The Adaptive Digestive System of The Migratory Locust in Response to Plant Defensive Protease Inhibitors. Sci Rep. 2016;6:32460.
Spit, J., Holtof, M., Badisco, L., Vergauwen, L., Vogel, E., Knapen, D., & Vanden Broeck, J. (2016). Transcriptional Analysis of The Adaptive Digestive System of The Migratory Locust in Response to Plant Defensive Protease Inhibitors. Scientific Reports, 6, 32460. https://doi.org/10.1038/srep32460
Spit J, et al. Transcriptional Analysis of the Adaptive Digestive System of the Migratory Locust in Response to Plant Defensive Protease Inhibitors. Sci Rep. 2016 09 1;6:32460. PubMed PMID: 27581362.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional Analysis of The Adaptive Digestive System of The Migratory Locust in Response to Plant Defensive Protease Inhibitors. AU - Spit,Jornt, AU - Holtof,Michiel, AU - Badisco,Liesbet, AU - Vergauwen,Lucia, AU - Vogel,Elise, AU - Knapen,Dries, AU - Vanden Broeck,Jozef, Y1 - 2016/09/01/ PY - 2016/06/06/received PY - 2016/08/05/accepted PY - 2016/9/2/entrez PY - 2016/9/2/pubmed PY - 2018/5/17/medline SP - 32460 EP - 32460 JF - Scientific reports JO - Sci Rep VL - 6 N2 - Herbivorous insects evolved adaptive mechanisms to compensate for the presence of plant defensive protease inhibitors (PI) in their food. The underlying regulatory mechanisms of these compensatory responses remain largely elusive. In the current study, we investigated the initiation of this adaptive response in the migratory locust, Locusta migratoria, via microarray analysis of gut tissues. Four hours after dietary uptake of PIs, 114 and 150 transcripts were respectively found up- or downregulated. The results suggest a quick trade-off between compensating for potential loss of digestive activity on the one hand, and stress tolerance, defense, and structural integrity of the gut on the other hand. We additionally addressed the role of a group of related upregulated hexamerin-like proteins in the PI-induced response. Simultaneous knockdown of corresponding transcripts by means of RNA interference resulted in a reduced capacity of the locust nymphs to cope with the effects of PI. Moreover, since insect hexamerins have been shown to bind Juvenile Hormone (JH), we also investigated the effect of JH on the proteolytic digestion in L. migratoria. Our results indicate that JH has a stimulatory effect on the expression of three homologous chymotrypsin genes, while knocking down the JH receptor (methoprene tolerant) led to opposite effects. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/27581362/Transcriptional_Analysis_of_The_Adaptive_Digestive_System_of_The_Migratory_Locust_in_Response_to_Plant_Defensive_Protease_Inhibitors_ DB - PRIME DP - Unbound Medicine ER -