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Characterization of a liposome-based artificial skin membrane for in vitro permeation studies using Franz diffusion cell device.
J Liposome Res 2017; 27(4):302-311JL

Abstract

A prerequisite for successful transdermal or dermal drug therapy is the drug ability to penetration through the skin, especially stratum corneum (SC). The most acceptable technique for measuring skin permeation in vitro is the application of both the Franz diffusion cell device and the skin model. In the skin model, a liposome-based artificial skin membrane (LASM) consisting of tight layers of liposomes immobilized on a filter was prepared and characterized. Using porcine ear skin, rat skin and Strat-M™ artificial membrane as control, the LASM was then evaluated in permeation studies with five active compounds: ferulic acid, paeoniflorin, albiflorin, tetrahydrocolumbamine, and tetrahydropalmatine. The scanning electron microscope images demonstrated complete filling of the membrane pores with lipids and the formation of a continuous liposomal coating. The contents of egg phosphatidylcholine (EPC) and cholesterol in LASM were measured to be 12.08 ± 0.18 and 4.41 ± 0.04 mg/cm2, respectively. Moreover, revealed by the measurement of electrical resistance, the LASM remains intact for at least 12 h with the incubation of 20% ethanol. The results of permeation studies demonstrated a good correlation (r2 = 0.9743, r = 0.9871) of Papp values between the drugs' permeation through LASM and porcine ear skin. In addition, by ATR-FTIR analysis, a slighter shift of CH2 stretching frequency between LASM and porcine ear skin was observed compared with the shift between Strat-M™ membrane and porcine ear skin. In summary, for the first time, the LASM has been proved to be a valuable alternative to porcine ear skin in permeation studies using Franz diffusion cell device.

Authors+Show Affiliations

a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine , Nanjing , China and. b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine , Nanjing , China and. b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine , Nanjing , China and. b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine , Nanjing , China and. b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine , Nanjing , China and. b Pharmaceutical Research Laboratory, School of Pharmacy, Nanjing University of Chinese Medicine , Nanjing , China.a Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine , Nanjing , China and.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27581379

Citation

Zhang, Hui, et al. "Characterization of a Liposome-based Artificial Skin Membrane for in Vitro Permeation Studies Using Franz Diffusion Cell Device." Journal of Liposome Research, vol. 27, no. 4, 2017, pp. 302-311.
Zhang H, Zhu X, Shen J, et al. Characterization of a liposome-based artificial skin membrane for in vitro permeation studies using Franz diffusion cell device. J Liposome Res. 2017;27(4):302-311.
Zhang, H., Zhu, X., Shen, J., Xu, H., Ma, M., Gu, W., ... Duan, J. (2017). Characterization of a liposome-based artificial skin membrane for in vitro permeation studies using Franz diffusion cell device. Journal of Liposome Research, 27(4), pp. 302-311. doi:10.1080/08982104.2016.1231205.
Zhang H, et al. Characterization of a Liposome-based Artificial Skin Membrane for in Vitro Permeation Studies Using Franz Diffusion Cell Device. J Liposome Res. 2017;27(4):302-311. PubMed PMID: 27581379.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of a liposome-based artificial skin membrane for in vitro permeation studies using Franz diffusion cell device. AU - Zhang,Hui, AU - Zhu,Xuemin, AU - Shen,Jingjing, AU - Xu,Haiheng, AU - Ma,Min, AU - Gu,Wei, AU - Jiang,Qiudong, AU - Chen,Jun, AU - Duan,Jinao, Y1 - 2016/09/28/ PY - 2016/9/2/pubmed PY - 2018/11/13/medline PY - 2016/9/2/entrez KW - Artificial skin membrane KW - liposomes KW - permeation studies SP - 302 EP - 311 JF - Journal of liposome research JO - J Liposome Res VL - 27 IS - 4 N2 - A prerequisite for successful transdermal or dermal drug therapy is the drug ability to penetration through the skin, especially stratum corneum (SC). The most acceptable technique for measuring skin permeation in vitro is the application of both the Franz diffusion cell device and the skin model. In the skin model, a liposome-based artificial skin membrane (LASM) consisting of tight layers of liposomes immobilized on a filter was prepared and characterized. Using porcine ear skin, rat skin and Strat-M™ artificial membrane as control, the LASM was then evaluated in permeation studies with five active compounds: ferulic acid, paeoniflorin, albiflorin, tetrahydrocolumbamine, and tetrahydropalmatine. The scanning electron microscope images demonstrated complete filling of the membrane pores with lipids and the formation of a continuous liposomal coating. The contents of egg phosphatidylcholine (EPC) and cholesterol in LASM were measured to be 12.08 ± 0.18 and 4.41 ± 0.04 mg/cm2, respectively. Moreover, revealed by the measurement of electrical resistance, the LASM remains intact for at least 12 h with the incubation of 20% ethanol. The results of permeation studies demonstrated a good correlation (r2 = 0.9743, r = 0.9871) of Papp values between the drugs' permeation through LASM and porcine ear skin. In addition, by ATR-FTIR analysis, a slighter shift of CH2 stretching frequency between LASM and porcine ear skin was observed compared with the shift between Strat-M™ membrane and porcine ear skin. In summary, for the first time, the LASM has been proved to be a valuable alternative to porcine ear skin in permeation studies using Franz diffusion cell device. SN - 1532-2394 UR - https://www.unboundmedicine.com/medline/citation/27581379/Characterization_of_a_liposome_based_artificial_skin_membrane_for_in_vitro_permeation_studies_using_Franz_diffusion_cell_device_ L2 - http://www.tandfonline.com/doi/full/10.1080/08982104.2016.1231205 DB - PRIME DP - Unbound Medicine ER -