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Nutritional supplements modulate fluorescent protein-bound advanced glycation endproducts and digestive enzymes related to type 2 diabetes mellitus.
BMC Complement Altern Med. 2016 Sep 01; 16:338.BC

Abstract

BACKGROUND

Chronic hyperglycemia enhances the formation of advanced glycation endproducts (AGEs) and reactive oxygen species (ROS), contributing to diabetic complications. Thus, controlling blood glucose levels, inhibiting the formation of AGEs and reducing ROS are key therapeutic targets in early stage type 2 diabetes.

METHODS

The inhibitory effects of seven commercial liquid nutritional supplements against carbohydrate hydrolysing enzymes, α-amylase and α-glucosidase, was determined by dinitrosalicylic (DNS) reagent and p-nitrophenyl-α-D-glucopyranoside solution, respectively. Antiglycation activity was determined using the formation of fluorescent protein-bound AGEs. Total phenolic and flavonoid content and antioxidant properties (1,1-diphenyl-2-picrylhydrazyl antioxidant activity (DPPH) and ferric reducing antioxidant power (FRAP)) were determined for correlation among these components and inhibitory activities.

RESULTS

Samoan noni juice showed the greatest inhibitory effects against α-amylase, whereas chlorophyll extracts showed the greatest inhibitory effect against α-glucosidase. Inhibition of α-glucosidase correlated with TFC (r(2) = 0.766; p < 0.01) and FRAP (r(2) = 0.750; p < 0.01) whereas no correlation was observed for α-amylase inhibition. All supplements inhibited fluorescent protein-bound AGEs, with the greatest effect exerted by Olive Leaf Extract, Blood Sugar Support (IC50 = 0.5 mg/ml). The IC50 values negatively correlated with TPC (r(2) = -0.707; p < 0.001) and DPPH scavenging activities (r(2) = 0.515; p < 0.05).

CONCLUSION

The findings of this study highlight the potential of liquid nutritional supplements in managing and treating type 2 diabetes mellitus.

Authors+Show Affiliations

School of Pharmacy and Medical Sciences, University of the South Australia, Adelaide, SA, 5001, Australia.School of Pharmacy and Medical Sciences, University of the South Australia, Adelaide, SA, 5001, Australia. permal.deo@unisa.edu.au.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27585435

Citation

Koch, Emily R., and Permal Deo. "Nutritional Supplements Modulate Fluorescent Protein-bound Advanced Glycation Endproducts and Digestive Enzymes Related to Type 2 Diabetes Mellitus." BMC Complementary and Alternative Medicine, vol. 16, 2016, p. 338.
Koch ER, Deo P. Nutritional supplements modulate fluorescent protein-bound advanced glycation endproducts and digestive enzymes related to type 2 diabetes mellitus. BMC Complement Altern Med. 2016;16:338.
Koch, E. R., & Deo, P. (2016). Nutritional supplements modulate fluorescent protein-bound advanced glycation endproducts and digestive enzymes related to type 2 diabetes mellitus. BMC Complementary and Alternative Medicine, 16, 338. https://doi.org/10.1186/s12906-016-1329-0
Koch ER, Deo P. Nutritional Supplements Modulate Fluorescent Protein-bound Advanced Glycation Endproducts and Digestive Enzymes Related to Type 2 Diabetes Mellitus. BMC Complement Altern Med. 2016 Sep 1;16:338. PubMed PMID: 27585435.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nutritional supplements modulate fluorescent protein-bound advanced glycation endproducts and digestive enzymes related to type 2 diabetes mellitus. AU - Koch,Emily R, AU - Deo,Permal, Y1 - 2016/09/01/ PY - 2016/04/26/received PY - 2016/08/26/accepted PY - 2016/9/3/entrez PY - 2016/9/3/pubmed PY - 2017/2/17/medline KW - Antiglycation KW - Antioxidant activities KW - Flavonoids KW - Liquid nutritional supplements KW - Phenolic KW - α-amylase KW - α-glucosidase SP - 338 EP - 338 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 16 N2 - BACKGROUND: Chronic hyperglycemia enhances the formation of advanced glycation endproducts (AGEs) and reactive oxygen species (ROS), contributing to diabetic complications. Thus, controlling blood glucose levels, inhibiting the formation of AGEs and reducing ROS are key therapeutic targets in early stage type 2 diabetes. METHODS: The inhibitory effects of seven commercial liquid nutritional supplements against carbohydrate hydrolysing enzymes, α-amylase and α-glucosidase, was determined by dinitrosalicylic (DNS) reagent and p-nitrophenyl-α-D-glucopyranoside solution, respectively. Antiglycation activity was determined using the formation of fluorescent protein-bound AGEs. Total phenolic and flavonoid content and antioxidant properties (1,1-diphenyl-2-picrylhydrazyl antioxidant activity (DPPH) and ferric reducing antioxidant power (FRAP)) were determined for correlation among these components and inhibitory activities. RESULTS: Samoan noni juice showed the greatest inhibitory effects against α-amylase, whereas chlorophyll extracts showed the greatest inhibitory effect against α-glucosidase. Inhibition of α-glucosidase correlated with TFC (r(2) = 0.766; p < 0.01) and FRAP (r(2) = 0.750; p < 0.01) whereas no correlation was observed for α-amylase inhibition. All supplements inhibited fluorescent protein-bound AGEs, with the greatest effect exerted by Olive Leaf Extract, Blood Sugar Support (IC50 = 0.5 mg/ml). The IC50 values negatively correlated with TPC (r(2) = -0.707; p < 0.001) and DPPH scavenging activities (r(2) = 0.515; p < 0.05). CONCLUSION: The findings of this study highlight the potential of liquid nutritional supplements in managing and treating type 2 diabetes mellitus. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/27585435/Nutritional_supplements_modulate_fluorescent_protein_bound_advanced_glycation_endproducts_and_digestive_enzymes_related_to_type_2_diabetes_mellitus_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-016-1329-0 DB - PRIME DP - Unbound Medicine ER -