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In vitro analysis of partially hydrolyzed guar gum fermentation on identified gut microbiota.
Anaerobe. 2016 Dec; 42:60-66.A

Abstract

BACKGROUND

Prebiotic dietary fibers resist digestion in the upper gastrointestinal tract and allow for stimulation of bacteria in the distal intestine and colon. Stimulation of bacteria among different individuals varies greatly, depending on a wide range of variables.

OBJECTIVE

To determine the range of differences in response between individuals, a preclinical in vitro fermentation was conducted with six fecal donors. The primary objective was to compare the fecal microbiota of six individuals at baseline, 12 h and 24 h post-exposure to partially hydrolyzed guar gum (PHGG).

METHOD

Fecal donations were collected from six healthy individuals consuming a non-specific Western diet, free of antibiotic treatments in the past year, not affected by any GI diseases and not consuming any probiotic or prebiotic supplements. Fecal samples were exposed to 0.5 g of PHGG and measured for bacterial changes at 0, 12 and 24 h based on 16S rRNA sequencing.

RESULTS

Parabacteroides increased from 3.48% of sequence reads to 10.62% of sequence reads after 24 h (p = 0.0181) and Bacteroidetes increased from 45.89% of sequence reads to 50.29% of sequence reads (p = 0.0008).

CONCLUSIONS

PHGG stimulates growth of Parabacteroides, a genus of bacteria that have been inversely associated with IBS and ulcerative colitis. PHGG provides stimulation of beneficial Bacteroidetes (Bacteroides and Parabacteroides), which may be correlated with many positive health markers and outcomes. PHGG is a prebiotic dietary fiber that is readily fermentable.

Authors+Show Affiliations

Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Ave St. Paul, MN 55108, United States.Informatics Institute, University of Minnesota, 101 Pleasant St, Minneapolis, MN 55455, United States.Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Ave St. Paul, MN 55108, United States. Electronic address: jslavin@umn.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27585552

Citation

Carlson, Justin, et al. "In Vitro Analysis of Partially Hydrolyzed Guar Gum Fermentation On Identified Gut Microbiota." Anaerobe, vol. 42, 2016, pp. 60-66.
Carlson J, Gould T, Slavin J. In vitro analysis of partially hydrolyzed guar gum fermentation on identified gut microbiota. Anaerobe. 2016;42:60-66.
Carlson, J., Gould, T., & Slavin, J. (2016). In vitro analysis of partially hydrolyzed guar gum fermentation on identified gut microbiota. Anaerobe, 42, 60-66. https://doi.org/10.1016/j.anaerobe.2016.08.006
Carlson J, Gould T, Slavin J. In Vitro Analysis of Partially Hydrolyzed Guar Gum Fermentation On Identified Gut Microbiota. Anaerobe. 2016;42:60-66. PubMed PMID: 27585552.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro analysis of partially hydrolyzed guar gum fermentation on identified gut microbiota. AU - Carlson,Justin, AU - Gould,Trevor, AU - Slavin,Joanne, Y1 - 2016/08/30/ PY - 2016/06/14/received PY - 2016/08/22/revised PY - 2016/08/22/accepted PY - 2016/11/5/pubmed PY - 2017/1/17/medline PY - 2016/11/7/entrez KW - Fermentation KW - Microbiome KW - Microbiota KW - PHGG KW - Prebiotic SP - 60 EP - 66 JF - Anaerobe JO - Anaerobe VL - 42 N2 - BACKGROUND: Prebiotic dietary fibers resist digestion in the upper gastrointestinal tract and allow for stimulation of bacteria in the distal intestine and colon. Stimulation of bacteria among different individuals varies greatly, depending on a wide range of variables. OBJECTIVE: To determine the range of differences in response between individuals, a preclinical in vitro fermentation was conducted with six fecal donors. The primary objective was to compare the fecal microbiota of six individuals at baseline, 12 h and 24 h post-exposure to partially hydrolyzed guar gum (PHGG). METHOD: Fecal donations were collected from six healthy individuals consuming a non-specific Western diet, free of antibiotic treatments in the past year, not affected by any GI diseases and not consuming any probiotic or prebiotic supplements. Fecal samples were exposed to 0.5 g of PHGG and measured for bacterial changes at 0, 12 and 24 h based on 16S rRNA sequencing. RESULTS: Parabacteroides increased from 3.48% of sequence reads to 10.62% of sequence reads after 24 h (p = 0.0181) and Bacteroidetes increased from 45.89% of sequence reads to 50.29% of sequence reads (p = 0.0008). CONCLUSIONS: PHGG stimulates growth of Parabacteroides, a genus of bacteria that have been inversely associated with IBS and ulcerative colitis. PHGG provides stimulation of beneficial Bacteroidetes (Bacteroides and Parabacteroides), which may be correlated with many positive health markers and outcomes. PHGG is a prebiotic dietary fiber that is readily fermentable. SN - 1095-8274 UR - https://www.unboundmedicine.com/medline/citation/27585552/In_vitro_analysis_of_partially_hydrolyzed_guar_gum_fermentation_on_identified_gut_microbiota_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1075-9964(16)30097-X DB - PRIME DP - Unbound Medicine ER -