TY - JOUR T1 - Impact of Phosphate, Potassium, Yeast Extract, and Trace Metals on Chitosan and Metabolite Production by Mucor indicus. AU - Safaei,Zahra, AU - Karimi,Keikhosro, AU - Zamani,Akram, Y1 - 2016/08/30/ PY - 2016/07/21/received PY - 2016/08/16/revised PY - 2016/08/22/accepted PY - 2016/9/3/entrez PY - 2016/9/3/pubmed PY - 2017/3/24/medline KW - Mucor indicus KW - chitosan KW - ethanol KW - phosphate KW - potassium KW - trace metals KW - yeast extract JF - International journal of molecular sciences JO - Int J Mol Sci VL - 17 IS - 9 N2 - In this study the effects of phosphate, potassium, yeast extract, and trace metals on the growth of Mucor indicus and chitosan, chitin, and metabolite production by the fungus were investigated. Maximum yield of chitosan (0.32 g/g cell wall) was obtained in a phosphate-free medium. Reversely, cell growth and ethanol formation by the fungus were positively affected in the presence of phosphate. In a phosphate-free medium, the highest chitosan content (0.42 g/g cell wall) and cell growth (0.66 g/g sugar) were obtained at 2.5 g/L of KOH. Potassium concentration had no significant effect on ethanol and glycerol yields. The presence of trace metals significantly increased the chitosan yield at an optimal phosphate and potassium concentration (0.50 g/g cell wall). By contrast, production of ethanol by the fungus was negatively affected (0.33 g/g sugars). A remarkable increase in chitin and decrease in chitosan were observed in the absence of yeast extract and concentrations lower than 2 g/L. The maximum chitosan yield of 51% cell wall was obtained at 5 g/L of yeast extract when the medium contained no phosphate, 2.5 g/L KOH, and 1 mL/L trace metal solution. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/27589726/Impact_of_Phosphate_Potassium_Yeast_Extract_and_Trace_Metals_on_Chitosan_and_Metabolite_Production_by_Mucor_indicus_ DB - PRIME DP - Unbound Medicine ER -