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A quantitative proteomic approach to highlight Phragmites sp. adaptation mechanisms to chemical stress induced by a textile dyeing pollutant.
Sci Total Environ. 2016 Dec 15; 573:788-798.ST

Abstract

Phragmites sp. is present worldwide in treatment wetlands though the mechanisms involved in the phytoremediation remain unclear. In this study a quantitative proteomic approach was used to study the prompt response and adaptation of Phragmites to the textile dyeing pollutant, Acid Orange 7 (AO7). Previously, it was demonstrated that AO7 could be successfully removed from wastewater and mineralized in a constructed wetland planted with Phragmites sp. This azo dye is readily taken up by roots and transported to the plant aerial part by the xylem. Phragmites leaf samples were collected from a pilot scale vertical flow constructed wetland after 0.25, 3.25 and 24.25h exposure to AO7 (400mgL-1) immediately after a watering cycle used as control. Leaf soluble protein extraction yielded an average of 1560 proteins in a broad pI range (pH3-10) by two-dimensional gel electrophoresis. A time course comparative analysis of leaf proteome revealed that 40 proteins had a differential abundance compared to control (p<0.05) within a 3.25h period. After 24.25h in contact with AO7, leaf proteome was similar to control. Adaptation to AO7 involved proteins related with cellular signalling (calreticulin, Ras-related protein Rab11D and 20S proteasome), energy production and conversion (adenosine triphosphate synthase beta subunit) carbohydrate transport and metabolism (phosphoglucose isomerase, fructose-bisphosphate aldolase, monodehydroascorbate reductase, frutockinase-1 and Hypothetical protein POPTR_0003s12000g and the Uncharacterized protein LOC100272772) and photosynthesis (sedoheptulose-1,7-bisphosphatase and ferredoxin-NADP+ reductase). Therefore, the quantitative proteomic approach used in this work indicates that mechanisms associated with stress cell signalling, energy production, carbohydrate transport and metabolism as well as proteins related with photosynthesis are key players in the initial chemical stress response in the phytoremediation process of AO7.

Authors+Show Affiliations

Centre for Natural Resources and the Environment (CERENA), Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. Electronic address: renata.ferreira@tecnico.ulisboa.pt.Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.Centre for Natural Resources and the Environment (CERENA), Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal; Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal; Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. Electronic address: isacoreia@tecnico.ulisboa.pt.Centre for Natural Resources and the Environment (CERENA), Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal; Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. Electronic address: susetedias@tecnico.ulisboa.pt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27592466

Citation

Ferreira, R A., et al. "A Quantitative Proteomic Approach to Highlight Phragmites Sp. Adaptation Mechanisms to Chemical Stress Induced By a Textile Dyeing Pollutant." The Science of the Total Environment, vol. 573, 2016, pp. 788-798.
Ferreira RA, Roma-Rodrigues C, Davies LC, et al. A quantitative proteomic approach to highlight Phragmites sp. adaptation mechanisms to chemical stress induced by a textile dyeing pollutant. Sci Total Environ. 2016;573:788-798.
Ferreira, R. A., Roma-Rodrigues, C., Davies, L. C., Sá-Correia, I., & Martins-Dias, S. (2016). A quantitative proteomic approach to highlight Phragmites sp. adaptation mechanisms to chemical stress induced by a textile dyeing pollutant. The Science of the Total Environment, 573, 788-798. https://doi.org/10.1016/j.scitotenv.2016.08.169
Ferreira RA, et al. A Quantitative Proteomic Approach to Highlight Phragmites Sp. Adaptation Mechanisms to Chemical Stress Induced By a Textile Dyeing Pollutant. Sci Total Environ. 2016 Dec 15;573:788-798. PubMed PMID: 27592466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A quantitative proteomic approach to highlight Phragmites sp. adaptation mechanisms to chemical stress induced by a textile dyeing pollutant. AU - Ferreira,R A, AU - Roma-Rodrigues,C, AU - Davies,L C, AU - Sá-Correia,I, AU - Martins-Dias,S, Y1 - 2016/09/01/ PY - 2016/07/26/received PY - 2016/08/23/revised PY - 2016/08/23/accepted PY - 2016/10/18/pubmed PY - 2018/3/22/medline PY - 2016/9/5/entrez KW - Acid Orange 7 KW - Azo dyes KW - Constructed wetlands KW - Photosynthesis KW - Phytoremediation KW - Signalling SP - 788 EP - 798 JF - The Science of the total environment JO - Sci Total Environ VL - 573 N2 - Phragmites sp. is present worldwide in treatment wetlands though the mechanisms involved in the phytoremediation remain unclear. In this study a quantitative proteomic approach was used to study the prompt response and adaptation of Phragmites to the textile dyeing pollutant, Acid Orange 7 (AO7). Previously, it was demonstrated that AO7 could be successfully removed from wastewater and mineralized in a constructed wetland planted with Phragmites sp. This azo dye is readily taken up by roots and transported to the plant aerial part by the xylem. Phragmites leaf samples were collected from a pilot scale vertical flow constructed wetland after 0.25, 3.25 and 24.25h exposure to AO7 (400mgL-1) immediately after a watering cycle used as control. Leaf soluble protein extraction yielded an average of 1560 proteins in a broad pI range (pH3-10) by two-dimensional gel electrophoresis. A time course comparative analysis of leaf proteome revealed that 40 proteins had a differential abundance compared to control (p<0.05) within a 3.25h period. After 24.25h in contact with AO7, leaf proteome was similar to control. Adaptation to AO7 involved proteins related with cellular signalling (calreticulin, Ras-related protein Rab11D and 20S proteasome), energy production and conversion (adenosine triphosphate synthase beta subunit) carbohydrate transport and metabolism (phosphoglucose isomerase, fructose-bisphosphate aldolase, monodehydroascorbate reductase, frutockinase-1 and Hypothetical protein POPTR_0003s12000g and the Uncharacterized protein LOC100272772) and photosynthesis (sedoheptulose-1,7-bisphosphatase and ferredoxin-NADP+ reductase). Therefore, the quantitative proteomic approach used in this work indicates that mechanisms associated with stress cell signalling, energy production, carbohydrate transport and metabolism as well as proteins related with photosynthesis are key players in the initial chemical stress response in the phytoremediation process of AO7. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/27592466/A_quantitative_proteomic_approach_to_highlight_Phragmites_sp__adaptation_mechanisms_to_chemical_stress_induced_by_a_textile_dyeing_pollutant_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(16)31871-X DB - PRIME DP - Unbound Medicine ER -