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Novel single-stranded DNA binding protein-assisted fluorescence aptamer switch based on FRET for homogeneous detection of antibiotics.
Biosens Bioelectron. 2017 Jan 15; 87:508-513.BB

Abstract

Herein, a smart single-stranded DNA binding protein (SSB)-assisted fluorescence aptamer switch based on fluorescence resonance energy transfer (FRET) was designed. The FRET switch was synthesized by connecting SSB labeled quantum dots (QDs@SSB) as donor with aptamer (apt) labeled gold nanoparticles (AuNPs@apt) as acceptor, and it was employed for detecting chloramphenicol (CAP) in a homogenous solution. In the assay, the interaction between core-shell QDs@SSB and AuNPs@apt leads to a dramatic quenching (turning off). After adding CAP in the detection system, AuNPs@apt can bind the target specifically then separate QDs@SSB with AuNPs@apt-target, resulting in restoring the fluorescence intensity of QDs (turning on). Consequently, the fluorescence intensity recovers and the recovery extent can be used for detection of CAP in homogenous phase via optical responses. Under optimal conditions, the fluorescence intensity increased linearly with increasing concentrations of CAP from 0.005 to 100ngmL-1. The limit of this fluorescence aptamer switch was around 3pgmL-1 for CAP detection. When the analyte is changed, the assay can be applied to detect other targets only by changing relative aptamer in AuNPs@apt probe. Furthermore, it has potential to be served as a simple, sensitive and portable platform for antibiotic contaminants detection in biological and environmental samples.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Wang Y
Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
Gan N
Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China. Electronic address: ganning@nbu.edu.cn.
Zhou Y
Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
Li T
Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
Cao Y
Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
Chen Y
Deptartment of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210000, China.

MeSH

AnimalsAnti-Bacterial AgentsAptamers, NucleotideBiosensing TechniquesChloramphenicolDNA-Binding ProteinsFluorescence Resonance Energy TransferGoldLimit of DetectionMetal NanoparticlesMilkQuantum Dots

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

27596250

Citation

Wang, Ye, et al. "Novel Single-stranded DNA Binding Protein-assisted Fluorescence Aptamer Switch Based On FRET for Homogeneous Detection of Antibiotics." Biosensors & Bioelectronics, vol. 87, 2017, pp. 508-513.
Wang Y, Gan N, Zhou Y, et al. Novel single-stranded DNA binding protein-assisted fluorescence aptamer switch based on FRET for homogeneous detection of antibiotics. Biosens Bioelectron. 2017;87:508-513.
Wang, Y., Gan, N., Zhou, Y., Li, T., Cao, Y., & Chen, Y. (2017). Novel single-stranded DNA binding protein-assisted fluorescence aptamer switch based on FRET for homogeneous detection of antibiotics. Biosensors & Bioelectronics, 87, 508-513. https://doi.org/10.1016/j.bios.2016.08.107
Wang Y, et al. Novel Single-stranded DNA Binding Protein-assisted Fluorescence Aptamer Switch Based On FRET for Homogeneous Detection of Antibiotics. Biosens Bioelectron. 2017 Jan 15;87:508-513. PubMed PMID: 27596250.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel single-stranded DNA binding protein-assisted fluorescence aptamer switch based on FRET for homogeneous detection of antibiotics. AU - Wang,Ye, AU - Gan,Ning, AU - Zhou,You, AU - Li,Tianhua, AU - Cao,Yuting, AU - Chen,Yinji, Y1 - 2016/08/30/ PY - 2016/06/15/received PY - 2016/08/08/revised PY - 2016/08/30/accepted PY - 2016/9/7/pubmed PY - 2017/2/22/medline PY - 2016/9/7/entrez KW - Aptamer KW - Chloramphenicol KW - FRET switch KW - Gold nanoparticles KW - Quantum dots KW - Single strand DNA-binding protein SP - 508 EP - 513 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 87 N2 - Herein, a smart single-stranded DNA binding protein (SSB)-assisted fluorescence aptamer switch based on fluorescence resonance energy transfer (FRET) was designed. The FRET switch was synthesized by connecting SSB labeled quantum dots (QDs@SSB) as donor with aptamer (apt) labeled gold nanoparticles (AuNPs@apt) as acceptor, and it was employed for detecting chloramphenicol (CAP) in a homogenous solution. In the assay, the interaction between core-shell QDs@SSB and AuNPs@apt leads to a dramatic quenching (turning off). After adding CAP in the detection system, AuNPs@apt can bind the target specifically then separate QDs@SSB with AuNPs@apt-target, resulting in restoring the fluorescence intensity of QDs (turning on). Consequently, the fluorescence intensity recovers and the recovery extent can be used for detection of CAP in homogenous phase via optical responses. Under optimal conditions, the fluorescence intensity increased linearly with increasing concentrations of CAP from 0.005 to 100ngmL-1. The limit of this fluorescence aptamer switch was around 3pgmL-1 for CAP detection. When the analyte is changed, the assay can be applied to detect other targets only by changing relative aptamer in AuNPs@apt probe. Furthermore, it has potential to be served as a simple, sensitive and portable platform for antibiotic contaminants detection in biological and environmental samples. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/27596250/Novel_single_stranded_DNA_binding_protein_assisted_fluorescence_aptamer_switch_based_on_FRET_for_homogeneous_detection_of_antibiotics_ DB - PRIME DP - Unbound Medicine ER -