Tags

Type your tag names separated by a space and hit enter

Beneficial effect of Ageratum conyzoides Linn (Asteraceae) upon inflammatory response induced by carrageenan into the mice pleural cavity.
J Ethnopharmacol. 2016 Dec 24; 194:337-347.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Ageratum conyzoides Linn (Asteraceae), a tropical plant that is very common in West Africa and some parts of Asia and South America, has been used to treat inflammatory disorders. In Brazil, teas made from A. conyzoides L. are used as anti-inflammatory, analgesic and anti-diarrheic agents. Therefore, it is necessary to study the mechanism of anti-inflammatory action of A. conyzoides L. to support its medicinal use for treating inflammatory conditions. These studies will also support the development of effective pharmacological agents with potent anti-inflammatory properties.

AIM OF THE STUDY

To evaluate the anti-inflammatory effects of the crude extract (CE), its derived fractions: ethanol (EtOH-F), hexane (HEX-F), ethyl acetate (EtOAc-F) and dichloromethane (DCM-F) and isolated compounds, such as 5'-methoxy nobiletin (MeONOB), 1,2-benzopyrone and eupalestin, which are obtained from the aerial parts of A. conyzoides L.

MATERIALS AND METHODS

These evaluations were performed using an animal model of inflammation induced by carrageenan. The following inflammatory parameters were analysed: leukocyte influx, protein concentration of the exudate, myeloperoxidase (MPO), adenosine deaminase (ADA) and nitric oxide metabolites (NOx) concentrations, interleukin 10 (IL-10), interleukin 17A (IL-17A), interleukin 6 (IL-6), tumor necrosis factor (TNF), interferon gamma (IFN-γ) and phosphorylation of p65 subunit of NF-κB (p-p65 NF-κB), p38 mitogen-activated protein kinases (p-p38 MAPK) were also analysed.

RESULTS

CE, its EtOH-F, HEX-F, EtOAc-F and DCM-F and the isolated compounds, including MeONOB, 1,2-benzopyrone and eupalestin, significantly reduced leukocyte influx, protein concentration of the exudate, MPO, ADA, and NOx concentrations (p<0.05). CE, EtOH-F and isolated compounds significantly reduced IL-17A, IL-6, TNF and IFN-γ levels (p<0.05). CE, EtOH-F and isolated compound 1,2-benzopyrone also increased IL-10 levels (p<0.05). Isolated compounds, MeONOB, 1,2-benzopyrone and eupalestin, reduced p-p65 NF-κB and p-p38 MAPK (p<0.01).

CONCLUSIONS

This study demonstrates that A. conyzoides L. exerts its important anti-inflammatory properties by inhibiting leukocyte influx and protein concentration of the exudate, as well as reducing the levels of several pro-inflammatory mediators. The anti-inflammatory action of A. conyzoides L. may be because of the inhibition of p65 NF-κB and MAPK activation by the isolated compounds.

Authors+Show Affiliations

Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Florianópolis, Brazil.Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Florianópolis, Brazil.Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Florianópolis, Brazil.Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Florianópolis, Brazil.Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Florianópolis, Brazil.Department of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina, Florianópolis, Brazil.Department of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina, Florianópolis, Brazil.Department of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina, Florianópolis, Brazil.Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Florianópolis, Brazil. Electronic address: tania.frode@ufsc.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27596330

Citation

Vigil de Mello, Silvana Virgínia Gagliotti, et al. "Beneficial Effect of Ageratum Conyzoides Linn (Asteraceae) Upon Inflammatory Response Induced By Carrageenan Into the Mice Pleural Cavity." Journal of Ethnopharmacology, vol. 194, 2016, pp. 337-347.
Vigil de Mello SV, da Rosa JS, Facchin BM, et al. Beneficial effect of Ageratum conyzoides Linn (Asteraceae) upon inflammatory response induced by carrageenan into the mice pleural cavity. J Ethnopharmacol. 2016;194:337-347.
Vigil de Mello, S. V., da Rosa, J. S., Facchin, B. M., Luz, A. B., Vicente, G., Faqueti, L. G., Rosa, D. W., Biavatti, M. W., & Fröde, T. S. (2016). Beneficial effect of Ageratum conyzoides Linn (Asteraceae) upon inflammatory response induced by carrageenan into the mice pleural cavity. Journal of Ethnopharmacology, 194, 337-347. https://doi.org/10.1016/j.jep.2016.09.003
Vigil de Mello SV, et al. Beneficial Effect of Ageratum Conyzoides Linn (Asteraceae) Upon Inflammatory Response Induced By Carrageenan Into the Mice Pleural Cavity. J Ethnopharmacol. 2016 Dec 24;194:337-347. PubMed PMID: 27596330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Beneficial effect of Ageratum conyzoides Linn (Asteraceae) upon inflammatory response induced by carrageenan into the mice pleural cavity. AU - Vigil de Mello,Silvana Virgínia Gagliotti, AU - da Rosa,Julia Salvan, AU - Facchin,Bruno Matheus, AU - Luz,Ana Beatriz Gobbo, AU - Vicente,Geison, AU - Faqueti,Larissa Gabriela, AU - Rosa,Daniela Weingartner, AU - Biavatti,Maique Weber, AU - Fröde,Tânia Silvia, Y1 - 2016/09/03/ PY - 2016/03/07/received PY - 2016/08/29/revised PY - 2016/09/02/accepted PY - 2016/9/7/pubmed PY - 2017/4/22/medline PY - 2016/9/7/entrez KW - 1,2-Benzopyrone (PubChem CID: 323) KW - 5′-Methoxy nobiletin (PubChem CID: 72815) KW - Ageratum conyzoides Linn (Asteraceae) KW - Anti-inflammatory effects KW - Eupalestin (PubChem CID: 331149) KW - MAPK KW - NF-Kappa B KW - Pro-inflammatory mediators SP - 337 EP - 347 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 194 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Ageratum conyzoides Linn (Asteraceae), a tropical plant that is very common in West Africa and some parts of Asia and South America, has been used to treat inflammatory disorders. In Brazil, teas made from A. conyzoides L. are used as anti-inflammatory, analgesic and anti-diarrheic agents. Therefore, it is necessary to study the mechanism of anti-inflammatory action of A. conyzoides L. to support its medicinal use for treating inflammatory conditions. These studies will also support the development of effective pharmacological agents with potent anti-inflammatory properties. AIM OF THE STUDY: To evaluate the anti-inflammatory effects of the crude extract (CE), its derived fractions: ethanol (EtOH-F), hexane (HEX-F), ethyl acetate (EtOAc-F) and dichloromethane (DCM-F) and isolated compounds, such as 5'-methoxy nobiletin (MeONOB), 1,2-benzopyrone and eupalestin, which are obtained from the aerial parts of A. conyzoides L. MATERIALS AND METHODS: These evaluations were performed using an animal model of inflammation induced by carrageenan. The following inflammatory parameters were analysed: leukocyte influx, protein concentration of the exudate, myeloperoxidase (MPO), adenosine deaminase (ADA) and nitric oxide metabolites (NOx) concentrations, interleukin 10 (IL-10), interleukin 17A (IL-17A), interleukin 6 (IL-6), tumor necrosis factor (TNF), interferon gamma (IFN-γ) and phosphorylation of p65 subunit of NF-κB (p-p65 NF-κB), p38 mitogen-activated protein kinases (p-p38 MAPK) were also analysed. RESULTS: CE, its EtOH-F, HEX-F, EtOAc-F and DCM-F and the isolated compounds, including MeONOB, 1,2-benzopyrone and eupalestin, significantly reduced leukocyte influx, protein concentration of the exudate, MPO, ADA, and NOx concentrations (p<0.05). CE, EtOH-F and isolated compounds significantly reduced IL-17A, IL-6, TNF and IFN-γ levels (p<0.05). CE, EtOH-F and isolated compound 1,2-benzopyrone also increased IL-10 levels (p<0.05). Isolated compounds, MeONOB, 1,2-benzopyrone and eupalestin, reduced p-p65 NF-κB and p-p38 MAPK (p<0.01). CONCLUSIONS: This study demonstrates that A. conyzoides L. exerts its important anti-inflammatory properties by inhibiting leukocyte influx and protein concentration of the exudate, as well as reducing the levels of several pro-inflammatory mediators. The anti-inflammatory action of A. conyzoides L. may be because of the inhibition of p65 NF-κB and MAPK activation by the isolated compounds. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/27596330/Beneficial_effect_of_Ageratum_conyzoides_Linn__Asteraceae__upon_inflammatory_response_induced_by_carrageenan_into_the_mice_pleural_cavity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)30653-5 DB - PRIME DP - Unbound Medicine ER -