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Continuous and selective measurement of oxytocin and vasopressin using boron-doped diamond electrodes.
Sci Rep. 2016 09 07; 6:32429.SR

Abstract

The electrochemical detection of oxytocin using boron-doped diamond (BDD) electrodes was studied. Cyclic voltammetry of oxytocin in a phosphate buffer solution exhibits an oxidation peak at +0.7 V (vs. Ag/AgCl), which is attributable to oxidation of the phenolic group in the tyrosyl moiety. Furthermore, the linearity of the current peaks obtained in flow injection analysis (FIA) using BDD microelectrodes over the oxytocin concentration range from 0.1 to 10.0 μM with a detection limit of 50 nM (S/N = 3) was high (R(2) = 0.995). Although the voltammograms of oxytocin and vasopressin observed with an as-deposited BDD electrode, as well as with a cathodically-reduced BDD electrode, were similar, a clear distinction was observed with anodically-oxidized BDD electrodes due to the attractive interaction between vasopressin and the oxidized BDD surface. By means of this distinction, selective measurements using chronoamperometry combined with flow injection analysis at an optimized potential were demonstrated, indicating the possibility of making selective in situ or in vivo measurements of oxytocin.

Authors+Show Affiliations

Department of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan.Department of Chemistry, Faculty of Mathematics and Science, University of Indonesia, Kampus UI Depok, Jakarta 116424, Indonesia.Department of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan. JST-ACCEL, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27599852

Citation

Asai, Kai, et al. "Continuous and Selective Measurement of Oxytocin and Vasopressin Using Boron-doped Diamond Electrodes." Scientific Reports, vol. 6, 2016, p. 32429.
Asai K, Ivandini TA, Einaga Y. Continuous and selective measurement of oxytocin and vasopressin using boron-doped diamond electrodes. Sci Rep. 2016;6:32429.
Asai, K., Ivandini, T. A., & Einaga, Y. (2016). Continuous and selective measurement of oxytocin and vasopressin using boron-doped diamond electrodes. Scientific Reports, 6, 32429. https://doi.org/10.1038/srep32429
Asai K, Ivandini TA, Einaga Y. Continuous and Selective Measurement of Oxytocin and Vasopressin Using Boron-doped Diamond Electrodes. Sci Rep. 2016 09 7;6:32429. PubMed PMID: 27599852.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Continuous and selective measurement of oxytocin and vasopressin using boron-doped diamond electrodes. AU - Asai,Kai, AU - Ivandini,Tribidasari A, AU - Einaga,Yasuaki, Y1 - 2016/09/07/ PY - 2016/04/07/received PY - 2016/08/09/accepted PY - 2016/9/8/entrez PY - 2016/9/8/pubmed PY - 2018/5/16/medline SP - 32429 EP - 32429 JF - Scientific reports JO - Sci Rep VL - 6 N2 - The electrochemical detection of oxytocin using boron-doped diamond (BDD) electrodes was studied. Cyclic voltammetry of oxytocin in a phosphate buffer solution exhibits an oxidation peak at +0.7 V (vs. Ag/AgCl), which is attributable to oxidation of the phenolic group in the tyrosyl moiety. Furthermore, the linearity of the current peaks obtained in flow injection analysis (FIA) using BDD microelectrodes over the oxytocin concentration range from 0.1 to 10.0 μM with a detection limit of 50 nM (S/N = 3) was high (R(2) = 0.995). Although the voltammograms of oxytocin and vasopressin observed with an as-deposited BDD electrode, as well as with a cathodically-reduced BDD electrode, were similar, a clear distinction was observed with anodically-oxidized BDD electrodes due to the attractive interaction between vasopressin and the oxidized BDD surface. By means of this distinction, selective measurements using chronoamperometry combined with flow injection analysis at an optimized potential were demonstrated, indicating the possibility of making selective in situ or in vivo measurements of oxytocin. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/27599852/Continuous_and_selective_measurement_of_oxytocin_and_vasopressin_using_boron_doped_diamond_electrodes_ L2 - https://doi.org/10.1038/srep32429 DB - PRIME DP - Unbound Medicine ER -