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Quorum Quenching and Microbial Control through Phenolic Extract of Eugenia Uniflora Fruits.
J Food Sci. 2016 Oct; 81(10):M2538-M2544.JF

Abstract

We describe the characterization of the centesimal composition, mineral and phenolic content of Eugenia uniflora fruit and the determination of the antioxidant, antimicrobial and quorum quenching activities of the pulp phenolic extract. Centesimal composition was determined according to standard methods; trace elements were measured by total reflection X-ray fluorescence spectroscopy. The phenolic compounds were extracted by solid-phase chromatography and quantified by spectrophotometry. Antioxidant activity was determined by using 3 different methods. Antimicrobial activity was evaluated against a panel of foodborne microorganisms and antiquorum sensing activity in Chromobacterium violaceum was performed by measuring inhibition of quorum sensing dependent violacein production. The centesimal composition (per 100 g of pulp) was as follows: protein 3.68 ± 0.21 g, lipids 0.02 ± 0.03 g, carbohydrates 10.31 g and fiber 2.06 g. Trace elements (mg/g of pulp) were determined as: K 0.90, Ca 3.36, Fe 0.60, Zn 0.17, Cl 0.56, Cr 0.06, Ni 0.04, and Cu 0.07. The pulp is a source of phenolic compounds and presents antioxidant activity similar to other berries. The fruit phenolic extract inhibited all tested bacteria. We also found that the fruit phenolic extract at low subinhibitory concentrations inhibited up to 96% of violacein production in C. violaceum, likely due to the fruit's phenolic content. This study shows the contribution of E. uniflora phenolic compounds to the antioxidant, antimicrobial and the newly discovered quorum quenching activity, all of which could be used by the food and pharmaceutical industries to develop new functional products.

Authors+Show Affiliations

Dept. of Foods, School of Nutrition, Federal Univ. of Ouro Preto, Ouro Preto, MG, Brazil.Dept. of Foods, School of Nutrition, Federal Univ. of Ouro Preto, Ouro Preto, MG, Brazil.Dept. of Foods, School of Nutrition, Federal Univ. of Ouro Preto, Ouro Preto, MG, Brazil.Dept. of Foods, School of Nutrition, Federal Univ. of Ouro Preto, Ouro Preto, MG, Brazil.Dept. of Foods, School of Nutrition, Federal Univ. of Ouro Preto, Ouro Preto, MG, Brazil.Faculty of Pharmacy, Federal Univ. of Juiz de Fora, Governador Valadares, MG, Brazil.Chemistry Dept, Inst. of Physical and Biological Sciences, Federal Univ. of Ouro Preto, Ouro Preto, MG, Brazil.Dept. of Food and Experimental Nutrition, Food Research Center, Faculty of Pharmaceutical Sciences, Univ. of São Paulo, SP, Brazil. uelintonpinto@usp.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27603708

Citation

Rodrigues, Adeline Conceição, et al. "Quorum Quenching and Microbial Control Through Phenolic Extract of Eugenia Uniflora Fruits." Journal of Food Science, vol. 81, no. 10, 2016, pp. M2538-M2544.
Rodrigues AC, Zola FG, Ávila Oliveira B, et al. Quorum Quenching and Microbial Control through Phenolic Extract of Eugenia Uniflora Fruits. J Food Sci. 2016;81(10):M2538-M2544.
Rodrigues, A. C., Zola, F. G., Ávila Oliveira, B., Sacramento, N. T., da Silva, E. R., Bertoldi, M. C., Taylor, J. G., & Pinto, U. M. (2016). Quorum Quenching and Microbial Control through Phenolic Extract of Eugenia Uniflora Fruits. Journal of Food Science, 81(10), M2538-M2544. https://doi.org/10.1111/1750-3841.13431
Rodrigues AC, et al. Quorum Quenching and Microbial Control Through Phenolic Extract of Eugenia Uniflora Fruits. J Food Sci. 2016;81(10):M2538-M2544. PubMed PMID: 27603708.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quorum Quenching and Microbial Control through Phenolic Extract of Eugenia Uniflora Fruits. AU - Rodrigues,Adeline Conceição, AU - Zola,Flávia Guimarães, AU - Ávila Oliveira,Brígida D', AU - Sacramento,Nayara Thais Barbosa, AU - da Silva,Elis Regina, AU - Bertoldi,Michele Corrêa, AU - Taylor,Jason Guy, AU - Pinto,Uelinton Manoel, Y1 - 2016/09/07/ PY - 2016/05/05/received PY - 2016/07/08/revised PY - 2016/07/27/accepted PY - 2016/9/8/pubmed PY - 2017/5/4/medline PY - 2016/9/8/entrez KW - antimicrobial activity KW - antioxidant capacity KW - phenolic compounds KW - pitanga KW - quorum sensing SP - M2538 EP - M2544 JF - Journal of food science JO - J. Food Sci. VL - 81 IS - 10 N2 - We describe the characterization of the centesimal composition, mineral and phenolic content of Eugenia uniflora fruit and the determination of the antioxidant, antimicrobial and quorum quenching activities of the pulp phenolic extract. Centesimal composition was determined according to standard methods; trace elements were measured by total reflection X-ray fluorescence spectroscopy. The phenolic compounds were extracted by solid-phase chromatography and quantified by spectrophotometry. Antioxidant activity was determined by using 3 different methods. Antimicrobial activity was evaluated against a panel of foodborne microorganisms and antiquorum sensing activity in Chromobacterium violaceum was performed by measuring inhibition of quorum sensing dependent violacein production. The centesimal composition (per 100 g of pulp) was as follows: protein 3.68 ± 0.21 g, lipids 0.02 ± 0.03 g, carbohydrates 10.31 g and fiber 2.06 g. Trace elements (mg/g of pulp) were determined as: K 0.90, Ca 3.36, Fe 0.60, Zn 0.17, Cl 0.56, Cr 0.06, Ni 0.04, and Cu 0.07. The pulp is a source of phenolic compounds and presents antioxidant activity similar to other berries. The fruit phenolic extract inhibited all tested bacteria. We also found that the fruit phenolic extract at low subinhibitory concentrations inhibited up to 96% of violacein production in C. violaceum, likely due to the fruit's phenolic content. This study shows the contribution of E. uniflora phenolic compounds to the antioxidant, antimicrobial and the newly discovered quorum quenching activity, all of which could be used by the food and pharmaceutical industries to develop new functional products. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/27603708/Quorum_Quenching_and_Microbial_Control_through_Phenolic_Extract_of_Eugenia_Uniflora_Fruits_ L2 - https://doi.org/10.1111/1750-3841.13431 DB - PRIME DP - Unbound Medicine ER -