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Biochemical and transcriptomic analyses reveal different metabolite biosynthesis profiles among three color and developmental stages in 'Anji Baicha' (Camellia sinensis).
BMC Plant Biol. 2016 09 08; 16(1):195.BP

Abstract

BACKGROUND

The new shoots of the albino tea cultivar 'Anji Baicha' are yellow or white at low temperatures and turn green as the environmental temperatures increase during the early spring. 'Anji Baicha' metabolite profiles exhibit considerable variability over three color and developmental stages, especially regarding the carotenoid, chlorophyll, and theanine concentrations. Previous studies focused on physiological characteristics, gene expression differences, and variations in metabolite abundances in albino tea plant leaves at specific growth stages. However, the molecular mechanisms regulating metabolite biosynthesis in various color and developmental stages in albino tea leaves have not been fully characterized.

RESULTS

We used RNA-sequencing to analyze 'Anji Baicha' leaves at the yellow-green, albescent, and re-greening stages. The leaf transcriptomes differed considerably among the three stages. Functional classifications based on Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that differentially expressed unigenes were mainly related to metabolic pathways, biosynthesis of secondary metabolites, phenylpropanoid biosynthesis, and carbon fixation in photosynthetic organisms. Chemical analyses revealed higher β-carotene and theanine levels, but lower chlorophyll a levels, in the albescent stage than in the green stage. Furthermore, unigenes involved in carotenoid, chlorophyll, and theanine biosyntheses were identified, and the expression patterns of the differentially expressed unigenes in these biosynthesis pathways were characterized. Through co-expression analyses, we identified the key genes in these pathways. These genes may be responsible for the metabolite biosynthesis differences among the different leaf color and developmental stages of 'Anji Baicha' tea plants.

CONCLUSIONS

Our study presents the results of transcriptomic and biochemical analyses of 'Anji Baicha' tea plants at various stages. The distinct transcriptome profiles for each color and developmental stage enabled us to identify changes to biosynthesis pathways and revealed the contributions of such variations to the albino phenotype of tea plants. Furthermore, comparisons of the transcriptomes and related metabolites helped clarify the molecular regulatory mechanisms underlying the secondary metabolic pathways in different stages.

Authors+Show Affiliations

Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, China. School of Agriculture and Food Science, Zhejiang Agriculture and Forestry University, Lin'an, Hangzhou, China.Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, China.Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, China.Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, China.Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, China.Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, China.Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, China.Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, China. liangchen@tricaas.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27609021

Citation

Li, Chun-Fang, et al. "Biochemical and Transcriptomic Analyses Reveal Different Metabolite Biosynthesis Profiles Among Three Color and Developmental Stages in 'Anji Baicha' (Camellia Sinensis)." BMC Plant Biology, vol. 16, no. 1, 2016, p. 195.
Li CF, Xu YX, Ma JQ, et al. Biochemical and transcriptomic analyses reveal different metabolite biosynthesis profiles among three color and developmental stages in 'Anji Baicha' (Camellia sinensis). BMC Plant Biol. 2016;16(1):195.
Li, C. F., Xu, Y. X., Ma, J. Q., Jin, J. Q., Huang, D. J., Yao, M. Z., Ma, C. L., & Chen, L. (2016). Biochemical and transcriptomic analyses reveal different metabolite biosynthesis profiles among three color and developmental stages in 'Anji Baicha' (Camellia sinensis). BMC Plant Biology, 16(1), 195. https://doi.org/10.1186/s12870-016-0885-2
Li CF, et al. Biochemical and Transcriptomic Analyses Reveal Different Metabolite Biosynthesis Profiles Among Three Color and Developmental Stages in 'Anji Baicha' (Camellia Sinensis). BMC Plant Biol. 2016 09 8;16(1):195. PubMed PMID: 27609021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biochemical and transcriptomic analyses reveal different metabolite biosynthesis profiles among three color and developmental stages in 'Anji Baicha' (Camellia sinensis). AU - Li,Chun-Fang, AU - Xu,Yan-Xia, AU - Ma,Jian-Qiang, AU - Jin,Ji-Qiang, AU - Huang,Dan-Juan, AU - Yao,Ming-Zhe, AU - Ma,Chun-Lei, AU - Chen,Liang, Y1 - 2016/09/08/ PY - 2016/08/08/received PY - 2016/08/31/accepted PY - 2016/9/10/entrez PY - 2016/9/10/pubmed PY - 2017/9/25/medline KW - Albino KW - Camellia sinensis KW - Carotenoids KW - Chlorophylls KW - RNA-seq KW - Tea plant KW - Theanine SP - 195 EP - 195 JF - BMC plant biology JO - BMC Plant Biol VL - 16 IS - 1 N2 - BACKGROUND: The new shoots of the albino tea cultivar 'Anji Baicha' are yellow or white at low temperatures and turn green as the environmental temperatures increase during the early spring. 'Anji Baicha' metabolite profiles exhibit considerable variability over three color and developmental stages, especially regarding the carotenoid, chlorophyll, and theanine concentrations. Previous studies focused on physiological characteristics, gene expression differences, and variations in metabolite abundances in albino tea plant leaves at specific growth stages. However, the molecular mechanisms regulating metabolite biosynthesis in various color and developmental stages in albino tea leaves have not been fully characterized. RESULTS: We used RNA-sequencing to analyze 'Anji Baicha' leaves at the yellow-green, albescent, and re-greening stages. The leaf transcriptomes differed considerably among the three stages. Functional classifications based on Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that differentially expressed unigenes were mainly related to metabolic pathways, biosynthesis of secondary metabolites, phenylpropanoid biosynthesis, and carbon fixation in photosynthetic organisms. Chemical analyses revealed higher β-carotene and theanine levels, but lower chlorophyll a levels, in the albescent stage than in the green stage. Furthermore, unigenes involved in carotenoid, chlorophyll, and theanine biosyntheses were identified, and the expression patterns of the differentially expressed unigenes in these biosynthesis pathways were characterized. Through co-expression analyses, we identified the key genes in these pathways. These genes may be responsible for the metabolite biosynthesis differences among the different leaf color and developmental stages of 'Anji Baicha' tea plants. CONCLUSIONS: Our study presents the results of transcriptomic and biochemical analyses of 'Anji Baicha' tea plants at various stages. The distinct transcriptome profiles for each color and developmental stage enabled us to identify changes to biosynthesis pathways and revealed the contributions of such variations to the albino phenotype of tea plants. Furthermore, comparisons of the transcriptomes and related metabolites helped clarify the molecular regulatory mechanisms underlying the secondary metabolic pathways in different stages. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/27609021/Biochemical_and_transcriptomic_analyses_reveal_different_metabolite_biosynthesis_profiles_among_three_color_and_developmental_stages_in_'Anji_Baicha'__Camellia_sinensis__ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-016-0885-2 DB - PRIME DP - Unbound Medicine ER -