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Antibacterial performance and in vivo diabetic wound healing of curcumin loaded gum tragacanth/poly(ε-caprolactone) electrospun nanofibers.

Abstract

In this study we describe the potential of electrospun curcumin-loaded poly(ε-caprolactone) (PCL)/gum tragacanth (GT) (PCL/GT/Cur) nanofibers for wound healing in diabetic rats. These scaffolds with antibacterial property against methicillin resistant Staphylococcus aureus as gram positive bacteria and extended spectrum β lactamase as gram negative bacteria were applied in two forms of acellular and cell-seeded for assessing their capability in healing full thickness wound on the dorsum of rats. After 15days, pathological study showed that the application of GT/PCL/Cur nanofibers caused markedly fast wound closure with well-formed granulation tissue dominated by fibroblast proliferation, collagen deposition, complete early regenerated epithelial layer and formation of sweat glands and hair follicles. No such appendage formation was observed in the untreated controls during this duration. Masson's trichrome staining confirmed the increased presence of collagen in the dermis of the nanofiber treated wounds on day 5 and 15, while the control wounds were largely devoid of collagen on day 5 and exhibited less collagen amount on day 15. Quantification analysis of scaffolds on day 5 confirmed that, tissue engineered scaffolds with increased amount of angiogenesis number, granulation tissue area (μ(2)), fibroblast number, and decreased epithelial gap (μ) can be more effective compared to GT/PCL/Cur nanofibers.

Authors+Show Affiliations

Textile Group, Engineering Department, University of Bonab, Bonab, Iran. Electronic address: m.ranjbar@bonabu.ac.ir.Tehran Heart Center, Tehran University of Medical Sciences, Iran.Textile engineering Department, Amirkabir University of Technology, Tehran, Iran.Cellular and Molecular Research Center, Iran University of Medical Science, Tehran, Iran.Department of Pathobiology, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27612816

Citation

Ranjbar-Mohammadi, Marziyeh, et al. "Antibacterial Performance and in Vivo Diabetic Wound Healing of Curcumin Loaded Gum Tragacanth/poly(ε-caprolactone) Electrospun Nanofibers." Materials Science & Engineering. C, Materials for Biological Applications, vol. 69, 2016, pp. 1183-91.
Ranjbar-Mohammadi M, Rabbani S, Bahrami SH, et al. Antibacterial performance and in vivo diabetic wound healing of curcumin loaded gum tragacanth/poly(ε-caprolactone) electrospun nanofibers. Mater Sci Eng C Mater Biol Appl. 2016;69:1183-91.
Ranjbar-Mohammadi, M., Rabbani, S., Bahrami, S. H., Joghataei, M. T., & Moayer, F. (2016). Antibacterial performance and in vivo diabetic wound healing of curcumin loaded gum tragacanth/poly(ε-caprolactone) electrospun nanofibers. Materials Science & Engineering. C, Materials for Biological Applications, 69, pp. 1183-91. doi:10.1016/j.msec.2016.08.032.
Ranjbar-Mohammadi M, et al. Antibacterial Performance and in Vivo Diabetic Wound Healing of Curcumin Loaded Gum Tragacanth/poly(ε-caprolactone) Electrospun Nanofibers. Mater Sci Eng C Mater Biol Appl. 2016 Dec 1;69:1183-91. PubMed PMID: 27612816.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial performance and in vivo diabetic wound healing of curcumin loaded gum tragacanth/poly(ε-caprolactone) electrospun nanofibers. AU - Ranjbar-Mohammadi,Marziyeh, AU - Rabbani,Shahram, AU - Bahrami,S Hajir, AU - Joghataei,M T, AU - Moayer,F, Y1 - 2016/08/13/ PY - 2016/06/17/received PY - 2016/07/23/revised PY - 2016/08/12/accepted PY - 2016/9/11/entrez PY - 2016/9/11/pubmed PY - 2017/4/21/medline KW - Curcumin KW - Diabetic wound healing KW - Gum tragacanth KW - Nanofibrous scaffolds KW - Poly(caprolactone) SP - 1183 EP - 91 JF - Materials science & engineering. C, Materials for biological applications JO - Mater Sci Eng C Mater Biol Appl VL - 69 N2 - In this study we describe the potential of electrospun curcumin-loaded poly(ε-caprolactone) (PCL)/gum tragacanth (GT) (PCL/GT/Cur) nanofibers for wound healing in diabetic rats. These scaffolds with antibacterial property against methicillin resistant Staphylococcus aureus as gram positive bacteria and extended spectrum β lactamase as gram negative bacteria were applied in two forms of acellular and cell-seeded for assessing their capability in healing full thickness wound on the dorsum of rats. After 15days, pathological study showed that the application of GT/PCL/Cur nanofibers caused markedly fast wound closure with well-formed granulation tissue dominated by fibroblast proliferation, collagen deposition, complete early regenerated epithelial layer and formation of sweat glands and hair follicles. No such appendage formation was observed in the untreated controls during this duration. Masson's trichrome staining confirmed the increased presence of collagen in the dermis of the nanofiber treated wounds on day 5 and 15, while the control wounds were largely devoid of collagen on day 5 and exhibited less collagen amount on day 15. Quantification analysis of scaffolds on day 5 confirmed that, tissue engineered scaffolds with increased amount of angiogenesis number, granulation tissue area (μ(2)), fibroblast number, and decreased epithelial gap (μ) can be more effective compared to GT/PCL/Cur nanofibers. SN - 1873-0191 UR - https://www.unboundmedicine.com/medline/citation/27612816/Antibacterial_performance_and_in_vivo_diabetic_wound_healing_of_curcumin_loaded_gum_tragacanth/poly_ε_caprolactone__electrospun_nanofibers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-4931(16)30813-X DB - PRIME DP - Unbound Medicine ER -