Tags

Type your tag names separated by a space and hit enter

Anti-diabetic and renoprotective effects of aliskiren in streptozotocin-induced diabetic nephropathy in female rats.
Naunyn Schmiedebergs Arch Pharmacol. 2016 Dec; 389(12):1315-1324.NS

Abstract

Since chronic kidney disease due to diabetic nephropathy (DN) is becoming an ever larger health burden worldwide, more effective therapies are desperately needed. In the present study, the anti-diabetic and renoprotective effects of aliskiren have been evaluated in streptozotocin (STZ)-induced DN in rats. DN was induced by a single intraperitoneal injection of STZ (65 mg/kg). Three weeks after STZ, rats were divided into four groups; normal, diabetic, diabetic treated with gliclazide (10 mg/kg/day) for 1 month, and diabetic treated with aliskiren (50 mg/kg/day) for 1 month. At the end of the experiment, mean arterial blood pressure and heart rate were recorded. Rats were then euthanized and serum was separated for determination of glucose, insulin, kidney function tests, superoxide dismutase activity (SOD), adiponectin, and tumor necrosis factor-alpha (TNF-α). One kidney was used for estimation of malondialdehyde (MDA), reduced glutathione (GSH), and nitric oxide (NO) contents. Other kidney was used for histopathological study and immunohistochemical measurement of caspase-3 and transforming growth factor beta (TGF-β). In addition, islets of Langerhans were isolated from normal rats by collagenase digestion technique for in vitro study. Aliskiren normalized STZ-induced hyperglycemia, increased insulin level both in vivo and in vitro, normalized kidney function tests and blood pressure, and alleviated STZ-induced kidney histopathological changes. This could be related to the ability of aliskiren toward preserving hemodynamic changes and alleviating oxidative stress and inflammatory and apoptotic markers induced by STZ in rats. However, aliskiren was more effective than gliclazide in relieving STZ-induced DN. These findings support the beneficial effect of aliskiren treatment in DN which could be attributed to its anti-diabetic, renoprotective, antioxidant, anti-inflammatory, and anti-apoptotic effects. Moreover, clinical studies are required to establish the effectiveness of aliskiren treatment in patients suffering from hypertension and diabetes.

Authors+Show Affiliations

Department of Pharmacology, National Organization for Drug Control and Research (NODCAR), Giza, 35521, Egypt. mahfozamal@gmail.com. Faculty of Pharmacy, Cairo University, Kasr El Aini St., Cairo, 11562, Egypt. mahfozamal@gmail.com.Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt.Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt.Department of Pharmacology, National Organization for Drug Control and Research (NODCAR), Giza, 35521, Egypt.Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

27612855

Citation

Mahfoz, Amal M., et al. "Anti-diabetic and Renoprotective Effects of Aliskiren in Streptozotocin-induced Diabetic Nephropathy in Female Rats." Naunyn-Schmiedeberg's Archives of Pharmacology, vol. 389, no. 12, 2016, pp. 1315-1324.
Mahfoz AM, El-Latif HA, Ahmed LA, et al. Anti-diabetic and renoprotective effects of aliskiren in streptozotocin-induced diabetic nephropathy in female rats. Naunyn Schmiedebergs Arch Pharmacol. 2016;389(12):1315-1324.
Mahfoz, A. M., El-Latif, H. A., Ahmed, L. A., Hassanein, N. M., & Shoka, A. A. (2016). Anti-diabetic and renoprotective effects of aliskiren in streptozotocin-induced diabetic nephropathy in female rats. Naunyn-Schmiedeberg's Archives of Pharmacology, 389(12), 1315-1324.
Mahfoz AM, et al. Anti-diabetic and Renoprotective Effects of Aliskiren in Streptozotocin-induced Diabetic Nephropathy in Female Rats. Naunyn Schmiedebergs Arch Pharmacol. 2016;389(12):1315-1324. PubMed PMID: 27612855.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-diabetic and renoprotective effects of aliskiren in streptozotocin-induced diabetic nephropathy in female rats. AU - Mahfoz,Amal M, AU - El-Latif,Hekma A Abd, AU - Ahmed,Lamiaa A, AU - Hassanein,Nahed M, AU - Shoka,Afaf A, Y1 - 2016/09/09/ PY - 2016/04/19/received PY - 2016/09/02/accepted PY - 2016/9/11/pubmed PY - 2017/2/24/medline PY - 2016/9/11/entrez KW - Aliskiren KW - Diabetic nephropathy KW - Gliclazide KW - Oxidative stress KW - Renin-angiotensin system SP - 1315 EP - 1324 JF - Naunyn-Schmiedeberg's archives of pharmacology JO - Naunyn Schmiedebergs Arch Pharmacol VL - 389 IS - 12 N2 - Since chronic kidney disease due to diabetic nephropathy (DN) is becoming an ever larger health burden worldwide, more effective therapies are desperately needed. In the present study, the anti-diabetic and renoprotective effects of aliskiren have been evaluated in streptozotocin (STZ)-induced DN in rats. DN was induced by a single intraperitoneal injection of STZ (65 mg/kg). Three weeks after STZ, rats were divided into four groups; normal, diabetic, diabetic treated with gliclazide (10 mg/kg/day) for 1 month, and diabetic treated with aliskiren (50 mg/kg/day) for 1 month. At the end of the experiment, mean arterial blood pressure and heart rate were recorded. Rats were then euthanized and serum was separated for determination of glucose, insulin, kidney function tests, superoxide dismutase activity (SOD), adiponectin, and tumor necrosis factor-alpha (TNF-α). One kidney was used for estimation of malondialdehyde (MDA), reduced glutathione (GSH), and nitric oxide (NO) contents. Other kidney was used for histopathological study and immunohistochemical measurement of caspase-3 and transforming growth factor beta (TGF-β). In addition, islets of Langerhans were isolated from normal rats by collagenase digestion technique for in vitro study. Aliskiren normalized STZ-induced hyperglycemia, increased insulin level both in vivo and in vitro, normalized kidney function tests and blood pressure, and alleviated STZ-induced kidney histopathological changes. This could be related to the ability of aliskiren toward preserving hemodynamic changes and alleviating oxidative stress and inflammatory and apoptotic markers induced by STZ in rats. However, aliskiren was more effective than gliclazide in relieving STZ-induced DN. These findings support the beneficial effect of aliskiren treatment in DN which could be attributed to its anti-diabetic, renoprotective, antioxidant, anti-inflammatory, and anti-apoptotic effects. Moreover, clinical studies are required to establish the effectiveness of aliskiren treatment in patients suffering from hypertension and diabetes. SN - 1432-1912 UR - https://www.unboundmedicine.com/medline/citation/27612855/Anti_diabetic_and_renoprotective_effects_of_aliskiren_in_streptozotocin_induced_diabetic_nephropathy_in_female_rats_ L2 - https://dx.doi.org/10.1007/s00210-016-1299-2 DB - PRIME DP - Unbound Medicine ER -