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Localization of methyl-branched ceramide [EOS] species within the long-periodicity phase in stratum corneum lipid model membranes: A neutron diffraction study.
Biochim Biophys Acta. 2016 11; 1858(11):2911-2922.BB

Abstract

The outermost layer of the mammalian skin, the stratum corneum (SC), is a very thin structure and realizes simultaneously the main barrier properties. The penetration barrier for xenobiotica is mostly represented by a complex lipid matrix. There is great interest in the subject of getting information about the arrangement of the lipids, which are mainly ceramides (CER), free fatty acids (FFA) and cholesterol (CHOL). SC lipid model membranes containing synthetically derived lipids in a non-physiological ratio were investigated. To compare the study to a former experiment, a methyl-branched ceramide [EOS] species in presence of the ultra-long chain CER[AP], CHOL and behenic acid (23/10/33/33, wt%) was applied. The membrane structure was studied using the very versatile technique of neutron diffraction. We were able to identify a long-periodicity phase (LPP) with a size of 114Å or 118Å with CER[EOS]-br in a ratio of >60wt% of the ceramides. Furthermore, we figured out two additional coexisting short-periodicity phases (SPP) with repeat distances of 48Å and 45Å, respectively. Partial deuterations of CER[EOS]-br and CER[AP] enabled the localization of the molecules within the multiphase system. CER[EOS]-d3 was present in the LPP, but absent in both SPP. CER[AP]-d3 was determined in both short phases but not localized within the LPP. Besides, we revealed influences of humidity and time with respect to the long-periodicity phase.

Authors+Show Affiliations

Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120 Halle/Saale, Germany.Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120 Halle/Saale, Germany.Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120 Halle/Saale, Germany.Institute of Soft Matter and Functional Materials, Helmholtz-Zentrum Berlin für Materialien und Energie, 14109 Berlin, Germany.Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120 Halle/Saale, Germany; DR. KADE Pharmazeutische Fabrik GmbH, 12277 Berlin, Germany.Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120 Halle/Saale, Germany; Institute of Applied Dermatopharmacy at the Martin Luther University Halle-Wittenberg, 06120 Halle/Saale, Germany. Electronic address: reinhard.neubert@pharmazie.uni-halle.de.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27614192

Citation

Eichner, Adina, et al. "Localization of Methyl-branched Ceramide [EOS] Species Within the Long-periodicity Phase in Stratum Corneum Lipid Model Membranes: a Neutron Diffraction Study." Biochimica Et Biophysica Acta, vol. 1858, no. 11, 2016, pp. 2911-2922.
Eichner A, Sonnenberger S, Dobner B, et al. Localization of methyl-branched ceramide [EOS] species within the long-periodicity phase in stratum corneum lipid model membranes: A neutron diffraction study. Biochim Biophys Acta. 2016;1858(11):2911-2922.
Eichner, A., Sonnenberger, S., Dobner, B., Hauβ, T., Schroeter, A., & Neubert, R. H. H. (2016). Localization of methyl-branched ceramide [EOS] species within the long-periodicity phase in stratum corneum lipid model membranes: A neutron diffraction study. Biochimica Et Biophysica Acta, 1858(11), 2911-2922. https://doi.org/10.1016/j.bbamem.2016.09.002
Eichner A, et al. Localization of Methyl-branched Ceramide [EOS] Species Within the Long-periodicity Phase in Stratum Corneum Lipid Model Membranes: a Neutron Diffraction Study. Biochim Biophys Acta. 2016;1858(11):2911-2922. PubMed PMID: 27614192.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Localization of methyl-branched ceramide [EOS] species within the long-periodicity phase in stratum corneum lipid model membranes: A neutron diffraction study. AU - Eichner,Adina, AU - Sonnenberger,Stefan, AU - Dobner,Bodo, AU - Hauβ,Thomas, AU - Schroeter,Annett, AU - Neubert,Reinhard H H, Y1 - 2016/09/07/ PY - 2016/06/20/received PY - 2016/08/25/revised PY - 2016/09/03/accepted PY - 2016/9/11/entrez PY - 2016/9/11/pubmed PY - 2017/6/3/medline KW - Ceramides KW - Lipid model membrane KW - Long-periodicity phase KW - Neutron diffraction KW - Short- periodicity phase KW - Stratum corneum SP - 2911 EP - 2922 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1858 IS - 11 N2 - The outermost layer of the mammalian skin, the stratum corneum (SC), is a very thin structure and realizes simultaneously the main barrier properties. The penetration barrier for xenobiotica is mostly represented by a complex lipid matrix. There is great interest in the subject of getting information about the arrangement of the lipids, which are mainly ceramides (CER), free fatty acids (FFA) and cholesterol (CHOL). SC lipid model membranes containing synthetically derived lipids in a non-physiological ratio were investigated. To compare the study to a former experiment, a methyl-branched ceramide [EOS] species in presence of the ultra-long chain CER[AP], CHOL and behenic acid (23/10/33/33, wt%) was applied. The membrane structure was studied using the very versatile technique of neutron diffraction. We were able to identify a long-periodicity phase (LPP) with a size of 114Å or 118Å with CER[EOS]-br in a ratio of >60wt% of the ceramides. Furthermore, we figured out two additional coexisting short-periodicity phases (SPP) with repeat distances of 48Å and 45Å, respectively. Partial deuterations of CER[EOS]-br and CER[AP] enabled the localization of the molecules within the multiphase system. CER[EOS]-d3 was present in the LPP, but absent in both SPP. CER[AP]-d3 was determined in both short phases but not localized within the LPP. Besides, we revealed influences of humidity and time with respect to the long-periodicity phase. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/27614192/Localization_of_methyl_branched_ceramide_[EOS]_species_within_the_long_periodicity_phase_in_stratum_corneum_lipid_model_membranes:_A_neutron_diffraction_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0005-2736(16)30291-7 DB - PRIME DP - Unbound Medicine ER -