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Gallotannin-Enriched Extract Isolated from Galla Rhois May Be a Functional Candidate with Laxative Effects for Treatment of Loperamide-Induced Constipation of SD Rats.
PLoS One. 2016; 11(9):e0161144.Plos

Abstract

Several natural products containing tannins are used as traditional medicines for treatment of constipation; however, their pharmacological mechanism is not well understood. The laxative effects of gallotannin-enriched extract isolated from Galla Rhois (GEGR) were investigated using a constipation model induced by loperamide (Lop) injection. After analysis for antioxidant activity of GEGR, alterations in the excretion parameters, histological structure, mucin secretion, and related protein levels were measured in the transverse colon of Sprague Dawley (SD) rats with Lop-induced constipation following treatment with 250, 500 and 1,000 mg/ml of GEGR. The number and weight of feces increased significantly by 48-79% and 128-159%, respectively, in the Lop+GEGR treated group relative to the Lop+vehicle treated group, while food intake and water consumption were maintained at a constant level. The thickness of mucosa, muscle and flat luminal surface, as well as the number of goblet cells and crypt of lieberkuhn were enhanced in the Lop+GEGR treated group. Moreover, mucin secretion increased significantly in a dose dependent manner in the Lop+GEGR treated group. Furthermore, the downstream signaling pathway of the muscarinic acetylcholine receptors (mAChR) M2 and M3 was recovered by GEGR treatment, although the expression level varied. The levels of Gα expression and inositol triphosphate (IP3) concentration were also recovered in the Lop+GEGR treated group relative to the Lop+vehicle treated group. The results of the present study provide strong evidence that tannins distributed in various medicinal plants are important candidates for improving chronic constipation induced by Lop treatment in animal models.

Authors+Show Affiliations

Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.Department of Organic Materials Science and Engineering, Pusan National University, Busan, Korea.College of Pharmacy, Chungbuk National University, Chungju, Korea.Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27618438

Citation

Kim, Ji Eun, et al. "Gallotannin-Enriched Extract Isolated From Galla Rhois May Be a Functional Candidate With Laxative Effects for Treatment of Loperamide-Induced Constipation of SD Rats." PloS One, vol. 11, no. 9, 2016, pp. e0161144.
Kim JE, Go J, Koh EK, et al. Gallotannin-Enriched Extract Isolated from Galla Rhois May Be a Functional Candidate with Laxative Effects for Treatment of Loperamide-Induced Constipation of SD Rats. PLoS One. 2016;11(9):e0161144.
Kim, J. E., Go, J., Koh, E. K., Song, S. H., Sung, J. E., Lee, H. A., Lee, Y. H., Hong, J. T., & Hwang, D. Y. (2016). Gallotannin-Enriched Extract Isolated from Galla Rhois May Be a Functional Candidate with Laxative Effects for Treatment of Loperamide-Induced Constipation of SD Rats. PloS One, 11(9), e0161144. https://doi.org/10.1371/journal.pone.0161144
Kim JE, et al. Gallotannin-Enriched Extract Isolated From Galla Rhois May Be a Functional Candidate With Laxative Effects for Treatment of Loperamide-Induced Constipation of SD Rats. PLoS One. 2016;11(9):e0161144. PubMed PMID: 27618438.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gallotannin-Enriched Extract Isolated from Galla Rhois May Be a Functional Candidate with Laxative Effects for Treatment of Loperamide-Induced Constipation of SD Rats. AU - Kim,Ji Eun, AU - Go,Jun, AU - Koh,Eun Kyoung, AU - Song,Sung Hwa, AU - Sung,Ji Eun, AU - Lee,Hyun Ah, AU - Lee,Young Hee, AU - Hong,Jin Tae, AU - Hwang,Dae Youn, Y1 - 2016/09/12/ PY - 2016/03/30/received PY - 2016/08/01/accepted PY - 2016/9/13/entrez PY - 2016/9/13/pubmed PY - 2017/8/3/medline SP - e0161144 EP - e0161144 JF - PloS one JO - PLoS One VL - 11 IS - 9 N2 - Several natural products containing tannins are used as traditional medicines for treatment of constipation; however, their pharmacological mechanism is not well understood. The laxative effects of gallotannin-enriched extract isolated from Galla Rhois (GEGR) were investigated using a constipation model induced by loperamide (Lop) injection. After analysis for antioxidant activity of GEGR, alterations in the excretion parameters, histological structure, mucin secretion, and related protein levels were measured in the transverse colon of Sprague Dawley (SD) rats with Lop-induced constipation following treatment with 250, 500 and 1,000 mg/ml of GEGR. The number and weight of feces increased significantly by 48-79% and 128-159%, respectively, in the Lop+GEGR treated group relative to the Lop+vehicle treated group, while food intake and water consumption were maintained at a constant level. The thickness of mucosa, muscle and flat luminal surface, as well as the number of goblet cells and crypt of lieberkuhn were enhanced in the Lop+GEGR treated group. Moreover, mucin secretion increased significantly in a dose dependent manner in the Lop+GEGR treated group. Furthermore, the downstream signaling pathway of the muscarinic acetylcholine receptors (mAChR) M2 and M3 was recovered by GEGR treatment, although the expression level varied. The levels of Gα expression and inositol triphosphate (IP3) concentration were also recovered in the Lop+GEGR treated group relative to the Lop+vehicle treated group. The results of the present study provide strong evidence that tannins distributed in various medicinal plants are important candidates for improving chronic constipation induced by Lop treatment in animal models. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/27618438/Gallotannin_Enriched_Extract_Isolated_from_Galla_Rhois_May_Be_a_Functional_Candidate_with_Laxative_Effects_for_Treatment_of_Loperamide_Induced_Constipation_of_SD_Rats_ DB - PRIME DP - Unbound Medicine ER -