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Determination of a CD4+CD25-FoxP3+ T cells subset in tumor-draining lymph nodes of colorectal cancer secreting IL-2 and IFN-γ.
Tumour Biol 2016; 37(11):14659-14666TB

Abstract

CD4+CD25-FoxP3+ cells are a newly recognized subset of T cells which was first reported in autoimmune diseases. In our previous study, this subset was detected in tumor-draining lymph nodes (TDLNs) of patients with breast cancer. As little is known about their function in TDLNs of cancer patients, in this study, their frequency as well as their ability to produce interleukin (IL)-2, IL-10, or interferon (IFN)-γ were investigated in TDLNs of colorectal cancer (CRC) patients. Mononuclear cells were isolated from lymph nodes of 13 patients with CRC using Ficoll-Hypaque gradient centrifugation. Cells were stimulated in vitro and stained with CD25, CD4, FoxP3, IFN-γ, IL-10, and IL-2 or isotype matched antibodies and subjected to flow cytometry. The frequency of CD4+CD25-FoxP3+CD127dim/- cells was significantly lower than CD4+CD25+FoxP3+CD127dim/- population in TDLNs of CRC patients. The percentage of CD127dim/- cells and also the MFI of FoxP3 expression was significantly lower in CD4+CD25-FoxP3+ in comparison with CD4+CD25+FoxP3+ population. Moreover, CD4+CD25-FoxP3+ cells contained higher percentages of IL-2- and IFN-γ-producing cells than CD4+CD25+FoxP3+ subpopulation. But, no difference was seen between two subsets in terms of IL-10 production. CD4+CD25-FoxP3+ cells in TDLNs of CRC patients had lower suppressive and higher effector properties in comparison with CD4+CD25+FoxP3+ conventional regulatory T cells.

Authors+Show Affiliations

Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, P.O. Box: 71345-1798, Shiraz, Iran. Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, P.O. Box: 71345-1798, Shiraz, Iran.Colorectal Research Center, Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.Colorectal Research Center, Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, P.O. Box: 71345-1798, Shiraz, Iran. ghaderia@sums.ac.ir. Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. ghaderia@sums.ac.ir.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27619682

Citation

Jafarinia, Morteza, et al. "Determination of a CD4+CD25-FoxP3+ T Cells Subset in Tumor-draining Lymph Nodes of Colorectal Cancer Secreting IL-2 and IFN-γ." Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine, vol. 37, no. 11, 2016, pp. 14659-14666.
Jafarinia M, Mehdipour F, Hosseini SV, et al. Determination of a CD4+CD25-FoxP3+ T cells subset in tumor-draining lymph nodes of colorectal cancer secreting IL-2 and IFN-γ. Tumour Biol. 2016;37(11):14659-14666.
Jafarinia, M., Mehdipour, F., Hosseini, S. V., Ghahramani, L., Hosseinzadeh, M., & Ghaderi, A. (2016). Determination of a CD4+CD25-FoxP3+ T cells subset in tumor-draining lymph nodes of colorectal cancer secreting IL-2 and IFN-γ. Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine, 37(11), pp. 14659-14666.
Jafarinia M, et al. Determination of a CD4+CD25-FoxP3+ T Cells Subset in Tumor-draining Lymph Nodes of Colorectal Cancer Secreting IL-2 and IFN-γ. Tumour Biol. 2016;37(11):14659-14666. PubMed PMID: 27619682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of a CD4+CD25-FoxP3+ T cells subset in tumor-draining lymph nodes of colorectal cancer secreting IL-2 and IFN-γ. AU - Jafarinia,Morteza, AU - Mehdipour,Fereshteh, AU - Hosseini,Seyed Vahid, AU - Ghahramani,Leila, AU - Hosseinzadeh,Masood, AU - Ghaderi,Abbas, Y1 - 2016/09/13/ PY - 2016/04/05/received PY - 2016/09/06/accepted PY - 2016/9/14/pubmed PY - 2017/2/9/medline PY - 2016/9/14/entrez KW - CD4+CD25−FoxP3+ cells KW - Colorectal cancer KW - Cytokine KW - Lymph node SP - 14659 EP - 14666 JF - Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine JO - Tumour Biol. VL - 37 IS - 11 N2 - CD4+CD25-FoxP3+ cells are a newly recognized subset of T cells which was first reported in autoimmune diseases. In our previous study, this subset was detected in tumor-draining lymph nodes (TDLNs) of patients with breast cancer. As little is known about their function in TDLNs of cancer patients, in this study, their frequency as well as their ability to produce interleukin (IL)-2, IL-10, or interferon (IFN)-γ were investigated in TDLNs of colorectal cancer (CRC) patients. Mononuclear cells were isolated from lymph nodes of 13 patients with CRC using Ficoll-Hypaque gradient centrifugation. Cells were stimulated in vitro and stained with CD25, CD4, FoxP3, IFN-γ, IL-10, and IL-2 or isotype matched antibodies and subjected to flow cytometry. The frequency of CD4+CD25-FoxP3+CD127dim/- cells was significantly lower than CD4+CD25+FoxP3+CD127dim/- population in TDLNs of CRC patients. The percentage of CD127dim/- cells and also the MFI of FoxP3 expression was significantly lower in CD4+CD25-FoxP3+ in comparison with CD4+CD25+FoxP3+ population. Moreover, CD4+CD25-FoxP3+ cells contained higher percentages of IL-2- and IFN-γ-producing cells than CD4+CD25+FoxP3+ subpopulation. But, no difference was seen between two subsets in terms of IL-10 production. CD4+CD25-FoxP3+ cells in TDLNs of CRC patients had lower suppressive and higher effector properties in comparison with CD4+CD25+FoxP3+ conventional regulatory T cells. SN - 1423-0380 UR - https://www.unboundmedicine.com/medline/citation/27619682/Determination_of_a_CD4+CD25_FoxP3+_T_cells_subset_in_tumor_draining_lymph_nodes_of_colorectal_cancer_secreting_IL_2_and_IFN_γ_ L2 - https://link.springer.com/article/10.1007/s13277-016-5345-y DB - PRIME DP - Unbound Medicine ER -