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[Study on the electrophsiological properties in the stria vascularis pericytes in cochlear of guinea pig].

Abstract

OBJECTIVE

The purpose of this paper was to study the electrophysiological properties and the type of potassium channels on cell membrane in the stria vascularis pericytes in cochlear of guinea pig.

METHODS

Firstly examined the expression of the stria vascularis pericytes by desmin, a marker of pericytes, in cochlear of guinea pig with immunofluorescent method. Using whole-cell patch clamp recording techniques to observe electrophysiological properties in the cochlear pericytes in stria vascularis of guinea pig.

RESULTS

Pericytes were predominately distributed in the capillaries of cochlea.The average membrane capacitance, resistance, and potential of a single pericyte in stria vascularis were(5.9±0.3)pF, (2.2±0.3)GΩ and (-30.9±1.2)mV, respectively by using patch clamp technique. In addition, the average current density of cochlear pericyte was voltage-sensitive (Vh from 0 to + 60 mV, in 20 mV steps). The pericytes exhibited outward current and this property could be blocked by TEA (tetraethylammonium) 1 mmol/L, a large-conductance calcium-activated potassium channel(BKCa)inhibitor and 4-AP (4-aminopyridine) 1 mmol/L, a voltage-dependent K(+) channels(KV) channel blocker. TEA blocked the outward current from (296.2±35.9)pA to (163.7±16.8)pA and 4-AP blocked the outward current from (248.7±39.8)pA to (158.0±38.0)pA.

CONCLUSION

These results suggest that pericytes in stria vascularis have BKCa and KV channels.

Authors+Show Affiliations

Department of Physiology, the Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University; Shihezi 832002, China.Department of Physiology, the Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University; Shihezi 832002, China.Department of Physiology, the Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University; Shihezi 832002, China.Department of Physiology, the Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University; Shihezi 832002, China.Department of Physiology, the Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University; Shihezi 832002, China.Department of Physiology, the Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University; Shihezi 832002, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

27625130

Citation

Liu, Y H., et al. "[Study On the Electrophsiological Properties in the Stria Vascularis Pericytes in Cochlear of Guinea Pig]." Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi = Chinese Journal of Otorhinolaryngology Head and Neck Surgery, vol. 51, no. 8, 2016, pp. 600-5.
Liu YH, Wang YP, Wang Y, et al. [Study on the electrophsiological properties in the stria vascularis pericytes in cochlear of guinea pig]. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2016;51(8):600-5.
Liu, Y. H., Wang, Y. P., Wang, Y., Ma, K. T., Si, J. Q., & Li, L. (2016). [Study on the electrophsiological properties in the stria vascularis pericytes in cochlear of guinea pig]. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi = Chinese Journal of Otorhinolaryngology Head and Neck Surgery, 51(8), 600-5. https://doi.org/10.3760/cma.j.issn.1673-0860.2016.08.008
Liu YH, et al. [Study On the Electrophsiological Properties in the Stria Vascularis Pericytes in Cochlear of Guinea Pig]. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2016 Aug 7;51(8):600-5. PubMed PMID: 27625130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study on the electrophsiological properties in the stria vascularis pericytes in cochlear of guinea pig]. AU - Liu,Y H, AU - Wang,Y P, AU - Wang,Y, AU - Ma,K T, AU - Si,J Q, AU - Li,L, PY - 2016/9/15/entrez PY - 2016/9/15/pubmed PY - 2017/1/19/medline SP - 600 EP - 5 JF - Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery JO - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi VL - 51 IS - 8 N2 - OBJECTIVE: The purpose of this paper was to study the electrophysiological properties and the type of potassium channels on cell membrane in the stria vascularis pericytes in cochlear of guinea pig. METHODS: Firstly examined the expression of the stria vascularis pericytes by desmin, a marker of pericytes, in cochlear of guinea pig with immunofluorescent method. Using whole-cell patch clamp recording techniques to observe electrophysiological properties in the cochlear pericytes in stria vascularis of guinea pig. RESULTS: Pericytes were predominately distributed in the capillaries of cochlea.The average membrane capacitance, resistance, and potential of a single pericyte in stria vascularis were(5.9±0.3)pF, (2.2±0.3)GΩ and (-30.9±1.2)mV, respectively by using patch clamp technique. In addition, the average current density of cochlear pericyte was voltage-sensitive (Vh from 0 to + 60 mV, in 20 mV steps). The pericytes exhibited outward current and this property could be blocked by TEA (tetraethylammonium) 1 mmol/L, a large-conductance calcium-activated potassium channel(BKCa)inhibitor and 4-AP (4-aminopyridine) 1 mmol/L, a voltage-dependent K(+) channels(KV) channel blocker. TEA blocked the outward current from (296.2±35.9)pA to (163.7±16.8)pA and 4-AP blocked the outward current from (248.7±39.8)pA to (158.0±38.0)pA. CONCLUSION: These results suggest that pericytes in stria vascularis have BKCa and KV channels. SN - 1673-0860 UR - https://www.unboundmedicine.com/medline/citation/27625130/[Study_on_the_electrophsiological_properties_in_the_stria_vascularis_pericytes_in_cochlear_of_guinea_pig]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=1673-0860&year=2016&vol=51&issue=8&fpage=600 DB - PRIME DP - Unbound Medicine ER -