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Application of a quantitative 1H-NMR (1H-qNMR) method for the determination of geniposidic acid and acteoside in Plantaginis semen.
J Nat Med. 2017 Jan; 71(1):315-320.JN

Abstract

A quantitative 1H-NMR method (1H-qNMR) was developed to determine the concentration of acteoside and geniposidic acid in Plantaginis semen, which is an important crude drug for diuretic purposes. The purity of geniposidic acid and acteoside was determined by the ratio of the intensity of the H-3 signal at δ 7.51 ppm or the H-7″ signal at δ 7.58 ppm in methanol-d 4 to that of a hexamethyldisilane (HMD) signal at 0.04 ppm, respectively. The concentration of HMD was corrected with the International System of Units traceability using potassium hydrogen phthalate of certified reference material grade. The geniposidic acid content in two batches of Plantaginis semen as determined by 1H-qNMR was found to be 0.84 and 1.00 %, and the acteoside content was determined to be 0.80 and 0.93 %. We demonstrated that this method is useful for the quantitative analysis of geniposidic acid and acteoside in Plantainis semen.

Authors+Show Affiliations

Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan. National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan.National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan. anagatsu@kinjo-u.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27631429

Citation

Tanaka, Rie, et al. "Application of a Quantitative 1H-NMR (1H-qNMR) Method for the Determination of Geniposidic Acid and Acteoside in Plantaginis Semen." Journal of Natural Medicines, vol. 71, no. 1, 2017, pp. 315-320.
Tanaka R, Inagaki R, Sugimoto N, et al. Application of a quantitative 1H-NMR (1H-qNMR) method for the determination of geniposidic acid and acteoside in Plantaginis semen. J Nat Med. 2017;71(1):315-320.
Tanaka, R., Inagaki, R., Sugimoto, N., Akiyama, H., & Nagatsu, A. (2017). Application of a quantitative 1H-NMR (1H-qNMR) method for the determination of geniposidic acid and acteoside in Plantaginis semen. Journal of Natural Medicines, 71(1), 315-320. https://doi.org/10.1007/s11418-016-1040-y
Tanaka R, et al. Application of a Quantitative 1H-NMR (1H-qNMR) Method for the Determination of Geniposidic Acid and Acteoside in Plantaginis Semen. J Nat Med. 2017;71(1):315-320. PubMed PMID: 27631429.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of a quantitative 1H-NMR (1H-qNMR) method for the determination of geniposidic acid and acteoside in Plantaginis semen. AU - Tanaka,Rie, AU - Inagaki,Risa, AU - Sugimoto,Naoki, AU - Akiyama,Hiroshi, AU - Nagatsu,Akito, Y1 - 2016/09/08/ PY - 2016/07/18/received PY - 2016/08/27/accepted PY - 2016/9/16/pubmed PY - 2017/3/23/medline PY - 2016/9/16/entrez KW - 1H-qNMR KW - Acteoside KW - Geniposidic acid KW - Plantaginis semen KW - Reagent purity SP - 315 EP - 320 JF - Journal of natural medicines JO - J Nat Med VL - 71 IS - 1 N2 - A quantitative 1H-NMR method (1H-qNMR) was developed to determine the concentration of acteoside and geniposidic acid in Plantaginis semen, which is an important crude drug for diuretic purposes. The purity of geniposidic acid and acteoside was determined by the ratio of the intensity of the H-3 signal at δ 7.51 ppm or the H-7″ signal at δ 7.58 ppm in methanol-d 4 to that of a hexamethyldisilane (HMD) signal at 0.04 ppm, respectively. The concentration of HMD was corrected with the International System of Units traceability using potassium hydrogen phthalate of certified reference material grade. The geniposidic acid content in two batches of Plantaginis semen as determined by 1H-qNMR was found to be 0.84 and 1.00 %, and the acteoside content was determined to be 0.80 and 0.93 %. We demonstrated that this method is useful for the quantitative analysis of geniposidic acid and acteoside in Plantainis semen. SN - 1861-0293 UR - https://www.unboundmedicine.com/medline/citation/27631429/Application_of_a_quantitative_1H_NMR__1H_qNMR__method_for_the_determination_of_geniposidic_acid_and_acteoside_in_Plantaginis_semen_ DB - PRIME DP - Unbound Medicine ER -