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The germin-like protein OsGLP2-1 enhances resistance to fungal blast and bacterial blight in rice.
Plant Mol Biol 2016; 92(4-5):411-423PM

Abstract

KEY MESSAGE

This is the first report that GLP gene (OsGLP2-1) is involved in panicle blast and bacterial blight resistance in rice. In addition to its resistance to blast and bacterial blight, OsGLP2-1 has also been reported to co-localize with a QTLs for sheath blight resistance in rice. These suggest that the disease resistance provided by OsGLP2-1 is quantitative and broad spectrum. Its good resistance to these major diseases in rice makes it to be a promising target in rice breeding. Rice (Oryza sativa) blast caused by Magnaporthe oryzae and bacterial blight caused by Xanthomonas oryzae pv. oryzae are the two most destructive rice diseases worldwide. Germin-like protein (GLP) gene family is one of the important defense gene families which have been reported to be involved in disease resistance in plants. Although GLP proteins have been demonstrated to positively regulate leaf blast resistance in rice, their involvement in resistance to panicle blast and bacterial blight, has not been reported. In this study, we reported that one of the rice GLP genes, OsGLP2-1, was significantly induced by blast fungus. Overexpression of OsGLP2-1 quantitatively enhanced resistance to leaf blast, panicle blast and bacterial blight. The temporal and spatial expression analysis revealed that OsGLP2-1is highly expressed in leaves and panicles and sub-localized in the cell wall. Compared with empty vector transformed (control) plants, the OsGLP2-1 overexpressing plants exhibited higher levels of H2O2 both before and after pathogen inoculation. Moreover, OsGLP2-1 was significantly induced by jasmonic acid (JA). Overexpression of OsGLP2-1 induced three well-characterized defense-related genes which are associated in JA-dependent pathway after pathogen infection. Higher endogenous level of JA was also identified in OsGLP2-1 overexpressing plants than in control plants both before and after pathogen inoculation. Together, these results suggest that OsGLP2-1 functions as a positive regulator to modulate disease resistance. Its good quantitative resistance to the two major diseases in rice makes it to be a promising target in rice breeding.

Authors+Show Affiliations

Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.Guangdong Key Laboratory of New Technology in Plant protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.Guangdong Key Laboratory of New Technology in Plant protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China.Guangdong Key Laboratory of New Technology in Plant protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China. gzzhuxy@tom.com.Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China. lbgz1009@163.com. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Wushan, Tianhe District, Guangzhou, 510640, China. lbgz1009@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27631432

Citation

Liu, Qing, et al. "The Germin-like Protein OsGLP2-1 Enhances Resistance to Fungal Blast and Bacterial Blight in Rice." Plant Molecular Biology, vol. 92, no. 4-5, 2016, pp. 411-423.
Liu Q, Yang J, Yan S, et al. The germin-like protein OsGLP2-1 enhances resistance to fungal blast and bacterial blight in rice. Plant Mol Biol. 2016;92(4-5):411-423.
Liu, Q., Yang, J., Yan, S., Zhang, S., Zhao, J., Wang, W., ... Liu, B. (2016). The germin-like protein OsGLP2-1 enhances resistance to fungal blast and bacterial blight in rice. Plant Molecular Biology, 92(4-5), pp. 411-423.
Liu Q, et al. The Germin-like Protein OsGLP2-1 Enhances Resistance to Fungal Blast and Bacterial Blight in Rice. Plant Mol Biol. 2016;92(4-5):411-423. PubMed PMID: 27631432.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The germin-like protein OsGLP2-1 enhances resistance to fungal blast and bacterial blight in rice. AU - Liu,Qing, AU - Yang,Jianyuan, AU - Yan,Shijuan, AU - Zhang,Shaohong, AU - Zhao,Junliang, AU - Wang,Wenjuan, AU - Yang,Tifeng, AU - Wang,Xiaofei, AU - Mao,Xingxue, AU - Dong,Jingfang, AU - Zhu,Xiaoyuan, AU - Liu,Bin, Y1 - 2016/09/15/ PY - 2015/12/03/received PY - 2016/08/01/accepted PY - 2016/10/27/pubmed PY - 2017/2/10/medline PY - 2016/9/16/entrez KW - Bacterial blight KW - H2O2 KW - Jasmonic acid KW - Oryza sativa KW - OsGLP2-1 KW - Rice blast SP - 411 EP - 423 JF - Plant molecular biology JO - Plant Mol. Biol. VL - 92 IS - 4-5 N2 - KEY MESSAGE: This is the first report that GLP gene (OsGLP2-1) is involved in panicle blast and bacterial blight resistance in rice. In addition to its resistance to blast and bacterial blight, OsGLP2-1 has also been reported to co-localize with a QTLs for sheath blight resistance in rice. These suggest that the disease resistance provided by OsGLP2-1 is quantitative and broad spectrum. Its good resistance to these major diseases in rice makes it to be a promising target in rice breeding. Rice (Oryza sativa) blast caused by Magnaporthe oryzae and bacterial blight caused by Xanthomonas oryzae pv. oryzae are the two most destructive rice diseases worldwide. Germin-like protein (GLP) gene family is one of the important defense gene families which have been reported to be involved in disease resistance in plants. Although GLP proteins have been demonstrated to positively regulate leaf blast resistance in rice, their involvement in resistance to panicle blast and bacterial blight, has not been reported. In this study, we reported that one of the rice GLP genes, OsGLP2-1, was significantly induced by blast fungus. Overexpression of OsGLP2-1 quantitatively enhanced resistance to leaf blast, panicle blast and bacterial blight. The temporal and spatial expression analysis revealed that OsGLP2-1is highly expressed in leaves and panicles and sub-localized in the cell wall. Compared with empty vector transformed (control) plants, the OsGLP2-1 overexpressing plants exhibited higher levels of H2O2 both before and after pathogen inoculation. Moreover, OsGLP2-1 was significantly induced by jasmonic acid (JA). Overexpression of OsGLP2-1 induced three well-characterized defense-related genes which are associated in JA-dependent pathway after pathogen infection. Higher endogenous level of JA was also identified in OsGLP2-1 overexpressing plants than in control plants both before and after pathogen inoculation. Together, these results suggest that OsGLP2-1 functions as a positive regulator to modulate disease resistance. Its good quantitative resistance to the two major diseases in rice makes it to be a promising target in rice breeding. SN - 1573-5028 UR - https://www.unboundmedicine.com/medline/citation/27631432/The_germin_like_protein_OsGLP2_1_enhances_resistance_to_fungal_blast_and_bacterial_blight_in_rice_ L2 - https://doi.org/10.1007/s11103-016-0521-4 DB - PRIME DP - Unbound Medicine ER -