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Improved Quantitation of Gluten in Wheat Starch for Celiac Disease Patients by Gel-Permeation High-Performance Liquid Chromatography with Fluorescence Detection (GP-HPLC-FLD).
J Agric Food Chem. 2016 Oct 12; 64(40):7622-7631.JA

Abstract

Purified wheat starch (WSt) is commonly used in gluten-free products for celiac disease (CD) patients. It is mostly well-tolerated, but doubts about its safety for CD patients persist. One reason may be that most ELISA kits primarily recognize the alcohol-soluble gliadin fraction of gluten, but insufficiently target the alcohol-insoluble glutenin fraction. To address this problem, a new sensitive method based on the sequential extraction of gliadins, glutenins, and gluten from WSt followed by gel-permeation high-performance liquid chromatography with fluorescence detection (GP-HPLC-FLD) was developed. It revealed that considerable amounts of glutenins were present in most WSt. The gluten contents quantitated by GP-HPLC-FLD as sum of gliadins and glutenins were higher than those by R5 ELISA (gluten as gliadin content multiplied by a factor of 2) in 19 out of 26 WSt. Despite its limited selectivity, GP-HPLC-FLD may be applied as confirmatory method to ELISA to quantitate gluten in WSt.

Authors+Show Affiliations

Deutsche Forschungsanstalt für Lebensmittelchemie, Leibniz Institut , Lise-Meitner-Straβe 34, 85354 Freising, Germany.Deutsche Forschungsanstalt für Lebensmittelchemie, Leibniz Institut , Lise-Meitner-Straβe 34, 85354 Freising, Germany.Deutsche Forschungsanstalt für Lebensmittelchemie, Leibniz Institut , Lise-Meitner-Straβe 34, 85354 Freising, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27633005

Citation

Scherf, Katharina Anne, et al. "Improved Quantitation of Gluten in Wheat Starch for Celiac Disease Patients By Gel-Permeation High-Performance Liquid Chromatography With Fluorescence Detection (GP-HPLC-FLD)." Journal of Agricultural and Food Chemistry, vol. 64, no. 40, 2016, pp. 7622-7631.
Scherf KA, Wieser H, Koehler P. Improved Quantitation of Gluten in Wheat Starch for Celiac Disease Patients by Gel-Permeation High-Performance Liquid Chromatography with Fluorescence Detection (GP-HPLC-FLD). J Agric Food Chem. 2016;64(40):7622-7631.
Scherf, K. A., Wieser, H., & Koehler, P. (2016). Improved Quantitation of Gluten in Wheat Starch for Celiac Disease Patients by Gel-Permeation High-Performance Liquid Chromatography with Fluorescence Detection (GP-HPLC-FLD). Journal of Agricultural and Food Chemistry, 64(40), 7622-7631.
Scherf KA, Wieser H, Koehler P. Improved Quantitation of Gluten in Wheat Starch for Celiac Disease Patients By Gel-Permeation High-Performance Liquid Chromatography With Fluorescence Detection (GP-HPLC-FLD). J Agric Food Chem. 2016 Oct 12;64(40):7622-7631. PubMed PMID: 27633005.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved Quantitation of Gluten in Wheat Starch for Celiac Disease Patients by Gel-Permeation High-Performance Liquid Chromatography with Fluorescence Detection (GP-HPLC-FLD). AU - Scherf,Katharina Anne, AU - Wieser,Herbert, AU - Koehler,Peter, Y1 - 2016/09/29/ PY - 2016/9/17/pubmed PY - 2017/4/13/medline PY - 2016/9/17/entrez KW - celiac disease KW - enzyme-linked immunosorbent assay (ELISA) KW - gel-permeation HPLC fluorescence detection KW - gliadin KW - gluten analysis KW - glutenin KW - wheat starch SP - 7622 EP - 7631 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 64 IS - 40 N2 - Purified wheat starch (WSt) is commonly used in gluten-free products for celiac disease (CD) patients. It is mostly well-tolerated, but doubts about its safety for CD patients persist. One reason may be that most ELISA kits primarily recognize the alcohol-soluble gliadin fraction of gluten, but insufficiently target the alcohol-insoluble glutenin fraction. To address this problem, a new sensitive method based on the sequential extraction of gliadins, glutenins, and gluten from WSt followed by gel-permeation high-performance liquid chromatography with fluorescence detection (GP-HPLC-FLD) was developed. It revealed that considerable amounts of glutenins were present in most WSt. The gluten contents quantitated by GP-HPLC-FLD as sum of gliadins and glutenins were higher than those by R5 ELISA (gluten as gliadin content multiplied by a factor of 2) in 19 out of 26 WSt. Despite its limited selectivity, GP-HPLC-FLD may be applied as confirmatory method to ELISA to quantitate gluten in WSt. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/27633005/Improved_Quantitation_of_Gluten_in_Wheat_Starch_for_Celiac_Disease_Patients_by_Gel_Permeation_High_Performance_Liquid_Chromatography_with_Fluorescence_Detection__GP_HPLC_FLD__ L2 - https://doi.org/10.1021/acs.jafc.6b02512 DB - PRIME DP - Unbound Medicine ER -