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Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors.
Stem Cell Reports. 2016 10 11; 7(4):619-634.SC

Abstract

Allografts of retinal pigment epithelial (RPE) cells have been considered for the treatment of ocular diseases. We recently started the transplantation of induced pluripotent stem cell (iPSC)-derived RPE cells for patients with age-related macular degeneration (autogenic grafts). However, there are at least two problems with this approach: (1) high cost, and (2) uselessness for acute patients. To resolve these issues, we established RPE cells from induced iPSCs in HLA homozygote donors. In vitro, human T cells directly recognized allogeneic iPSC-derived RPE cells that expressed HLA class I/II antigens. However, these T cells failed to respond to HLA-A, -B, and -DRB1-matched iPSC-derived RPE cells from HLA homozygous donors. Because of the lack of T cell response to iPSC-derived RPE cells from HLA homozygous donors, we can use these allogeneic iPSC-derived RPE cells in future clinical trials if the recipient and donor are HLA matched.

Authors+Show Affiliations

Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan.Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan; Department of Ophthalmology and Visual Science, Tokyo Medical and Dental University Graduate School of Medicine and Dental Sciences, Tokyo 113-8519, Japan.Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan.Department of Fundamental Cell Technology, Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan.Department of Fundamental Cell Technology, Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan; HLA Foundation Laboratory, Kyoto 600-8813, Japan.Department of Fundamental Cell Technology, Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan; HLA Foundation Laboratory, Kyoto 600-8813, Japan.Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan. Electronic address: mretina@cdb.riken.jp.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27641646

Citation

Sugita, Sunao, et al. "Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells From HLA Homozygous Donors." Stem Cell Reports, vol. 7, no. 4, 2016, pp. 619-634.
Sugita S, Iwasaki Y, Makabe K, et al. Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors. Stem Cell Reports. 2016;7(4):619-634.
Sugita, S., Iwasaki, Y., Makabe, K., Kimura, T., Futagami, T., Suegami, S., & Takahashi, M. (2016). Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors. Stem Cell Reports, 7(4), 619-634. https://doi.org/10.1016/j.stemcr.2016.08.011
Sugita S, et al. Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells From HLA Homozygous Donors. Stem Cell Reports. 2016 10 11;7(4):619-634. PubMed PMID: 27641646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors. AU - Sugita,Sunao, AU - Iwasaki,Yuko, AU - Makabe,Kenichi, AU - Kimura,Takafumi, AU - Futagami,Takaomi, AU - Suegami,Shinji, AU - Takahashi,Masayo, Y1 - 2016/09/15/ PY - 2016/04/19/received PY - 2016/07/25/revised PY - 2016/08/16/accepted PY - 2016/9/20/pubmed PY - 2017/11/29/medline PY - 2016/9/20/entrez SP - 619 EP - 634 JF - Stem cell reports JO - Stem Cell Reports VL - 7 IS - 4 N2 - Allografts of retinal pigment epithelial (RPE) cells have been considered for the treatment of ocular diseases. We recently started the transplantation of induced pluripotent stem cell (iPSC)-derived RPE cells for patients with age-related macular degeneration (autogenic grafts). However, there are at least two problems with this approach: (1) high cost, and (2) uselessness for acute patients. To resolve these issues, we established RPE cells from induced iPSCs in HLA homozygote donors. In vitro, human T cells directly recognized allogeneic iPSC-derived RPE cells that expressed HLA class I/II antigens. However, these T cells failed to respond to HLA-A, -B, and -DRB1-matched iPSC-derived RPE cells from HLA homozygous donors. Because of the lack of T cell response to iPSC-derived RPE cells from HLA homozygous donors, we can use these allogeneic iPSC-derived RPE cells in future clinical trials if the recipient and donor are HLA matched. SN - 2213-6711 UR - https://www.unboundmedicine.com/medline/citation/27641646/Lack_of_T_Cell_Response_to_iPSC_Derived_Retinal_Pigment_Epithelial_Cells_from_HLA_Homozygous_Donors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2213-6711(16)30178-3 DB - PRIME DP - Unbound Medicine ER -