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Enantiomer Separations by Capillary Electrophoresis.
Methods Mol Biol. 2016; 1483:277-99.MM

Abstract

Capillary electrophoresis (CE) is a versatile and flexible technique for analytical enantioseparations. This is due to the large variety of chiral selectors as well as the different operation modes including electrokinetic chromatography, micellar electrokinetic chromatography, and microemulsion electrokinetic chromatography. The chiral selector, which is added to the background electrolyte, represents a pseudostationary phase with its own electrophoretic mobility allowing a variety of different separation protocols. The present chapter briefly addresses the basic fundamentals of CE enantioseparations as well as the most frequently applied chiral selectors and separation modes. The practical example illustrates the separation of the enantiomers of a positively charged analyte using native and charged cyclodextrin derivatives as chiral selectors.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Scriba GK
Department of Pharmaceutical Chemistry, University of Jena, Philosophenweg 14, Jena, 07743, Germany. gerhard.scriba@uni-jena.de.
Harnisch H
Department of Pharmaceutical Chemistry, University of Jena, Philosophenweg 14, Jena, 07743, Germany.
Zhu Q
Department of Chemistry, Wichita State University, 1845 Fairmount St, Wichita, KS, 67260, USA.

MeSH

Chromatography, Micellar Electrokinetic CapillaryCyclodextrinsElectrophoresis, CapillaryStereoisomerism

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27645742

Citation

Scriba, Gerhard K E., et al. "Enantiomer Separations By Capillary Electrophoresis." Methods in Molecular Biology (Clifton, N.J.), vol. 1483, 2016, pp. 277-99.
Scriba GK, Harnisch H, Zhu Q. Enantiomer Separations by Capillary Electrophoresis. Methods Mol Biol. 2016;1483:277-99.
Scriba, G. K., Harnisch, H., & Zhu, Q. (2016). Enantiomer Separations by Capillary Electrophoresis. Methods in Molecular Biology (Clifton, N.J.), 1483, 277-99. https://doi.org/10.1007/978-1-4939-6403-1_15
Scriba GK, Harnisch H, Zhu Q. Enantiomer Separations By Capillary Electrophoresis. Methods Mol Biol. 2016;1483:277-99. PubMed PMID: 27645742.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enantiomer Separations by Capillary Electrophoresis. AU - Scriba,Gerhard K E, AU - Harnisch,Henrik, AU - Zhu,Qingfu, PY - 2016/9/21/entrez PY - 2016/9/21/pubmed PY - 2018/1/4/medline KW - Capillary electrophoresis KW - Chiral selector KW - Chiral separation KW - Enantiomer separation KW - Migration mode SP - 277 EP - 99 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 1483 N2 - Capillary electrophoresis (CE) is a versatile and flexible technique for analytical enantioseparations. This is due to the large variety of chiral selectors as well as the different operation modes including electrokinetic chromatography, micellar electrokinetic chromatography, and microemulsion electrokinetic chromatography. The chiral selector, which is added to the background electrolyte, represents a pseudostationary phase with its own electrophoretic mobility allowing a variety of different separation protocols. The present chapter briefly addresses the basic fundamentals of CE enantioseparations as well as the most frequently applied chiral selectors and separation modes. The practical example illustrates the separation of the enantiomers of a positively charged analyte using native and charged cyclodextrin derivatives as chiral selectors. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/27645742/Enantiomer_Separations_by_Capillary_Electrophoresis_ DB - PRIME DP - Unbound Medicine ER -