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Methicillin-resistant Staphylococcus aureus isolates with SCCmec type V and spa types t437 or t1081 associated to discordant susceptibility results between oxacillin and cefoxitin, Central Taiwan.
Diagn Microbiol Infect Dis. 2016 Dec; 86(4):405-411.DM

Abstract

Staphylococcus aureus isolates with discordant susceptibility results between oxacillin and cefoxitin obtained using automated microbiology systems are infrequently observed. From April 2013 to December 2014, 1956 methicillin-resistant S. aureus (MRSA) and 1761 methicillin-susceptible S. aureus isolates were obtained from different patients. Forty isolates (1.1% and 2% in case of S. aureus and MRSA, respectively) with discordant susceptibility results (oxacillin susceptible and cefoxitin resistant) and carrying mecA gene were obtained. Except 2 SCCmec type IV isolates, 38 MRSA isolates were all SCCmec type V (VT or non-VT), which were further divided into VT (n=22) and non-VT (n=16). The most common spa type in VT and non-VT isolates were t437 (n=19) and t1081 (n=13), respectively. Only 55% of patients received effective antimicrobial agents; 2 mortalities were not attributable to MRSA infection. Using standard agar dilution, 17 MRSA isolates (0.46% and 0.87% in case of S. aureus and MRSA, respectively) had oxacillin MIC in the susceptible ranges (oxacillin-susceptible MRSA [OS-MRSA]); all carried SCCmec type V (VT, n=8; non-VT, n=9). The most common spa-MLST types of OS-MRSA in VT and non-VT were t437-ST59 (n=4) and t1081-ST45 (n=7), respectively. Concomitant testing by both cefoxitin- and oxacillin-based methods is a practical strategy for OS-MRSA detection in the clinical laboratories. Continuous monitoring of OS-MRSA isolates is necessary to elucidate their impact in clinical infectious diseases.

Authors+Show Affiliations

Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan; Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan; Department of Nursing, Hungkuang University, Taichung, Taiwan; Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan.Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan; Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan.Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan.Department of Infectious Diseases, Children's Hospital, China Medical University Hospital, China Medical University, Taichung, Taiwan.Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan.Department of Laboratory Medicine, Chang-Gung Memorial Hospital, Linkou, Taoyuan, Taiwan; Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Kweu-Shan, Taoyuan, Taiwan.Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan; Department of Laboratory Medicine, Chang-Gung Memorial Hospital, Linkou, Taoyuan, Taiwan; Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Kweu-Shan, Taoyuan, Taiwan. Electronic address: janglu45@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27650515

Citation

Ho, Cheng-Mao, et al. "Methicillin-resistant Staphylococcus Aureus Isolates With SCCmec Type V and Spa Types T437 or T1081 Associated to Discordant Susceptibility Results Between Oxacillin and Cefoxitin, Central Taiwan." Diagnostic Microbiology and Infectious Disease, vol. 86, no. 4, 2016, pp. 405-411.
Ho CM, Lin CY, Ho MW, et al. Methicillin-resistant Staphylococcus aureus isolates with SCCmec type V and spa types t437 or t1081 associated to discordant susceptibility results between oxacillin and cefoxitin, Central Taiwan. Diagn Microbiol Infect Dis. 2016;86(4):405-411.
Ho, C. M., Lin, C. Y., Ho, M. W., Lin, H. C., Chen, C. J., Lin, L. C., & Lu, J. J. (2016). Methicillin-resistant Staphylococcus aureus isolates with SCCmec type V and spa types t437 or t1081 associated to discordant susceptibility results between oxacillin and cefoxitin, Central Taiwan. Diagnostic Microbiology and Infectious Disease, 86(4), 405-411. https://doi.org/10.1016/j.diagmicrobio.2016.08.025
Ho CM, et al. Methicillin-resistant Staphylococcus Aureus Isolates With SCCmec Type V and Spa Types T437 or T1081 Associated to Discordant Susceptibility Results Between Oxacillin and Cefoxitin, Central Taiwan. Diagn Microbiol Infect Dis. 2016;86(4):405-411. PubMed PMID: 27650515.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Methicillin-resistant Staphylococcus aureus isolates with SCCmec type V and spa types t437 or t1081 associated to discordant susceptibility results between oxacillin and cefoxitin, Central Taiwan. AU - Ho,Cheng-Mao, AU - Lin,Chien-Yu, AU - Ho,Mao-Wang, AU - Lin,Hsiao-Chuan, AU - Chen,Chao-Jung, AU - Lin,Lee-Chung, AU - Lu,Jang-Jih, Y1 - 2016/08/28/ PY - 2016/05/16/received PY - 2016/08/03/revised PY - 2016/08/24/accepted PY - 2016/11/5/pubmed PY - 2017/2/9/medline PY - 2016/9/22/entrez KW - Cefoxitin KW - MLST KW - OS-MRSA KW - Oxacillin KW - Staphylococcus aureus KW - mecA KW - mecC KW - spa SP - 405 EP - 411 JF - Diagnostic microbiology and infectious disease JO - Diagn. Microbiol. Infect. Dis. VL - 86 IS - 4 N2 - Staphylococcus aureus isolates with discordant susceptibility results between oxacillin and cefoxitin obtained using automated microbiology systems are infrequently observed. From April 2013 to December 2014, 1956 methicillin-resistant S. aureus (MRSA) and 1761 methicillin-susceptible S. aureus isolates were obtained from different patients. Forty isolates (1.1% and 2% in case of S. aureus and MRSA, respectively) with discordant susceptibility results (oxacillin susceptible and cefoxitin resistant) and carrying mecA gene were obtained. Except 2 SCCmec type IV isolates, 38 MRSA isolates were all SCCmec type V (VT or non-VT), which were further divided into VT (n=22) and non-VT (n=16). The most common spa type in VT and non-VT isolates were t437 (n=19) and t1081 (n=13), respectively. Only 55% of patients received effective antimicrobial agents; 2 mortalities were not attributable to MRSA infection. Using standard agar dilution, 17 MRSA isolates (0.46% and 0.87% in case of S. aureus and MRSA, respectively) had oxacillin MIC in the susceptible ranges (oxacillin-susceptible MRSA [OS-MRSA]); all carried SCCmec type V (VT, n=8; non-VT, n=9). The most common spa-MLST types of OS-MRSA in VT and non-VT were t437-ST59 (n=4) and t1081-ST45 (n=7), respectively. Concomitant testing by both cefoxitin- and oxacillin-based methods is a practical strategy for OS-MRSA detection in the clinical laboratories. Continuous monitoring of OS-MRSA isolates is necessary to elucidate their impact in clinical infectious diseases. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/27650515/Methicillin_resistant_Staphylococcus_aureus_isolates_with_SCCmec_type_V_and_spa_types_t437_or_t1081_associated_to_discordant_susceptibility_results_between_oxacillin_and_cefoxitin_Central_Taiwan_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(16)30265-6 DB - PRIME DP - Unbound Medicine ER -