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Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides.
Int J Exp Pathol. 2016 08; 97(4):303-309.IJ

Abstract

Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA-DQ2 or DQ8 molecules to gluten-sensitive T cells. A ω-gliadin/C-hordein peptide (QPFPQPEQPFPW) and a rye-derived secalin peptide (QPFPQPQQPIPQ) were proposed to be toxic in CD, as they yielded positive responses when assessed with peripheral blood T-cell clones derived from individuals with CD. We sought to assess the immunogenicity of the candidate peptides using gluten-sensitive T-cell lines obtained from CD small intestinal biopsies. We also sought to investigate the potential cross-reactivity of wheat gluten-sensitive T-cell lines with peptic-tryptic digested barley hordein (PTH) and rye secalin (PTS). Synthesised candidate peptides were deamidated with tissue transglutaminase (tTG). Gluten-sensitive T-cell lines were generated by culturing small intestinal biopsies from CD patients with peptic-tryptic gluten (PTG), PTH or PTS, along with autologous PBMCs for antigen presentation. The stimulation indices were determined by measuring the relative cellular proliferation via incorporation of 3 H-thymidine. The majority of T-cell lines reacted to the peptides studied. There was also cross-reactivity between wheat gluten-sensitive T-cell lines and the hordein, gliadin and secalin peptides. PTH, PTS, barley hordein and rye secalin-derived CD antigen-sensitive T-cell lines showed positive stimulation with PTG. ω-gliadin/C-hordein peptide and rye-derived peptide are immunogenic to gluten-sensitive T-cell lines and potentially present in wheat, rye and barley. Additional CD toxic peptides may be shared.

Authors+Show Affiliations

Department of Biotechnology, Kulliyyah of Science, International Islamic University Malaysia (IIUM), Kuantan, Malaysia.Division of Diabetes and Nutritional Sciences, Department of Gastroenterology, King's College London, Rayne Institute, St. Thomas' Hospital, London, UK.Division of Diabetes and Nutritional Sciences, Department of Gastroenterology, King's College London, Rayne Institute, St. Thomas' Hospital, London, UK.Division of Diabetes and Nutritional Sciences, Department of Gastroenterology, King's College London, Rayne Institute, St. Thomas' Hospital, London, UK.Division of Diabetes and Nutritional Sciences, Department of Gastroenterology, King's College London, Rayne Institute, St. Thomas' Hospital, London, UK. paul.ciclitira@kcl.ac.uk.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27659035

Citation

Wahab, Widya A., et al. "Coeliac Disease: Immunogenicity Studies of Barley Hordein and Rye Secalin-derived Peptides." International Journal of Experimental Pathology, vol. 97, no. 4, 2016, pp. 303-309.
Wahab WA, Šuligoj T, Ellis J, et al. Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides. Int J Exp Pathol. 2016;97(4):303-309.
Wahab, W. A., Šuligoj, T., Ellis, J., Côrtez-Real, B., & Ciclitira, P. J. (2016). Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides. International Journal of Experimental Pathology, 97(4), 303-309. https://doi.org/10.1111/iep.12199
Wahab WA, et al. Coeliac Disease: Immunogenicity Studies of Barley Hordein and Rye Secalin-derived Peptides. Int J Exp Pathol. 2016;97(4):303-309. PubMed PMID: 27659035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides. AU - Wahab,Widya A, AU - Šuligoj,Tanja, AU - Ellis,Julia, AU - Côrtez-Real,Beatriz, AU - Ciclitira,Paul J, Y1 - 2016/09/23/ PY - 2016/02/29/received PY - 2016/07/27/accepted PY - 2016/9/24/pubmed PY - 2017/7/18/medline PY - 2016/9/24/entrez KW - antigen presentation KW - coeliac disease KW - gluten KW - small intestinal T-cell lines SP - 303 EP - 309 JF - International journal of experimental pathology JO - Int J Exp Pathol VL - 97 IS - 4 N2 - Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA-DQ2 or DQ8 molecules to gluten-sensitive T cells. A ω-gliadin/C-hordein peptide (QPFPQPEQPFPW) and a rye-derived secalin peptide (QPFPQPQQPIPQ) were proposed to be toxic in CD, as they yielded positive responses when assessed with peripheral blood T-cell clones derived from individuals with CD. We sought to assess the immunogenicity of the candidate peptides using gluten-sensitive T-cell lines obtained from CD small intestinal biopsies. We also sought to investigate the potential cross-reactivity of wheat gluten-sensitive T-cell lines with peptic-tryptic digested barley hordein (PTH) and rye secalin (PTS). Synthesised candidate peptides were deamidated with tissue transglutaminase (tTG). Gluten-sensitive T-cell lines were generated by culturing small intestinal biopsies from CD patients with peptic-tryptic gluten (PTG), PTH or PTS, along with autologous PBMCs for antigen presentation. The stimulation indices were determined by measuring the relative cellular proliferation via incorporation of 3 H-thymidine. The majority of T-cell lines reacted to the peptides studied. There was also cross-reactivity between wheat gluten-sensitive T-cell lines and the hordein, gliadin and secalin peptides. PTH, PTS, barley hordein and rye secalin-derived CD antigen-sensitive T-cell lines showed positive stimulation with PTG. ω-gliadin/C-hordein peptide and rye-derived peptide are immunogenic to gluten-sensitive T-cell lines and potentially present in wheat, rye and barley. Additional CD toxic peptides may be shared. SN - 1365-2613 UR - https://www.unboundmedicine.com/medline/citation/27659035/Coeliac_disease:_immunogenicity_studies_of_barley_hordein_and_rye_secalin_derived_peptides_ L2 - https://doi.org/10.1111/iep.12199 DB - PRIME DP - Unbound Medicine ER -