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QTL mapping of Fusarium head blight resistance in three related durum wheat populations.
Theor Appl Genet. 2017 Jan; 130(1):13-27.TA

Abstract

KEY MESSAGE

The QTL Fhb1 was successfully introgressed and validated in three durum wheat populations. The novel germplasm and the QTL detected will support improvement of Fusarium resistance in durum wheat. Durum wheat (Triticum durum Desf.) is particularly susceptible to Fusarium head blight (FHB) and breeding for resistance is hampered by limited genetic variation within this species. To date, resistant sources are mainly available in a few wild relative tetraploid wheat accessions. In this study, the effect of the well-known hexaploid wheat (Triticum aestivum L.) quantitative trait locus (QTL) Fhb1 was assessed for the first time in durum wheat. Three F7-RIL mapping populations of about 100 lines were developed from crosses between the durum wheat experimental line DBC-480, which carries an Fhb1 introgression from Sumai-3, and the European T. durum cultivars Karur, Durobonus and SZD1029K. The RILs were evaluated in field experiments for FHB resistance in three seasons using spray inoculation and genotyped with SSR as well as genotyping-by-sequencing markers. QTL associated with FHB resistance were identified on chromosome arms 2BL, 3BS, 4AL, 4BS, 5AL and 6AS at which the resistant parent DBC-480 contributed the positive alleles. The QTL on 3BS was detected in all three populations centered at the Fhb1 interval. The Rht-B1 locus governing plant height was found to have a strong effect in modulating FHB severity in all populations. The negative effect of the semi-dwarf allele Rht-B1b on FHB resistance was compensated by combining with Fhb1 and additional resistance QTL. The successful deployment of Fhb1 in T. durum was further substantiated by assessing type 2 resistance in one population. The efficient introgression of Fhb1 represents a significant step forward for enhancing FHB resistance in durum wheat.

Authors+Show Affiliations

Department of Agrobiotechnology, Institute of Biotechnology in Plant Production, BOKU-University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Str. 20, 3430, Tulln, Austria. INRA, UBP, Genetics, Diversity and Ecophysiology of Cereals, GDEC, 63039, Clermont-Ferrand Cedex 2, France. Florimond-Desprez, 3 rue Florimond-Desprez, BP 41, 59242, Cappelle-en-Pevele, France.Department of Agrobiotechnology, Institute of Biotechnology in Plant Production, BOKU-University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Str. 20, 3430, Tulln, Austria.Department of Agrobiotechnology, Institute of Biotechnology in Plant Production, BOKU-University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Str. 20, 3430, Tulln, Austria.Department of Agrobiotechnology, Institute of Biotechnology in Plant Production, BOKU-University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Str. 20, 3430, Tulln, Austria.Department of Agrobiotechnology, Institute of Biotechnology in Plant Production, BOKU-University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Str. 20, 3430, Tulln, Austria.INRA, UBP, Genetics, Diversity and Ecophysiology of Cereals, GDEC, 63039, Clermont-Ferrand Cedex 2, France.Florimond-Desprez, 3 rue Florimond-Desprez, BP 41, 59242, Cappelle-en-Pevele, France.Department of Agrobiotechnology, Institute of Biotechnology in Plant Production, BOKU-University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Str. 20, 3430, Tulln, Austria. hermann.buerstmayr@boku.ac.at.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27662843

Citation

Prat, Noemie, et al. "QTL Mapping of Fusarium Head Blight Resistance in Three Related Durum Wheat Populations." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 130, no. 1, 2017, pp. 13-27.
Prat N, Guilbert C, Prah U, et al. QTL mapping of Fusarium head blight resistance in three related durum wheat populations. Theor Appl Genet. 2017;130(1):13-27.
Prat, N., Guilbert, C., Prah, U., Wachter, E., Steiner, B., Langin, T., Robert, O., & Buerstmayr, H. (2017). QTL mapping of Fusarium head blight resistance in three related durum wheat populations. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 130(1), 13-27. https://doi.org/10.1007/s00122-016-2785-0
Prat N, et al. QTL Mapping of Fusarium Head Blight Resistance in Three Related Durum Wheat Populations. Theor Appl Genet. 2017;130(1):13-27. PubMed PMID: 27662843.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - QTL mapping of Fusarium head blight resistance in three related durum wheat populations. AU - Prat,Noemie, AU - Guilbert,Camille, AU - Prah,Ursa, AU - Wachter,Elisabeth, AU - Steiner,Barbara, AU - Langin,Thierry, AU - Robert,Olivier, AU - Buerstmayr,Hermann, Y1 - 2016/09/23/ PY - 2016/06/28/received PY - 2016/09/03/accepted PY - 2016/9/25/pubmed PY - 2017/2/14/medline PY - 2016/9/25/entrez SP - 13 EP - 27 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 130 IS - 1 N2 - KEY MESSAGE: The QTL Fhb1 was successfully introgressed and validated in three durum wheat populations. The novel germplasm and the QTL detected will support improvement of Fusarium resistance in durum wheat. Durum wheat (Triticum durum Desf.) is particularly susceptible to Fusarium head blight (FHB) and breeding for resistance is hampered by limited genetic variation within this species. To date, resistant sources are mainly available in a few wild relative tetraploid wheat accessions. In this study, the effect of the well-known hexaploid wheat (Triticum aestivum L.) quantitative trait locus (QTL) Fhb1 was assessed for the first time in durum wheat. Three F7-RIL mapping populations of about 100 lines were developed from crosses between the durum wheat experimental line DBC-480, which carries an Fhb1 introgression from Sumai-3, and the European T. durum cultivars Karur, Durobonus and SZD1029K. The RILs were evaluated in field experiments for FHB resistance in three seasons using spray inoculation and genotyped with SSR as well as genotyping-by-sequencing markers. QTL associated with FHB resistance were identified on chromosome arms 2BL, 3BS, 4AL, 4BS, 5AL and 6AS at which the resistant parent DBC-480 contributed the positive alleles. The QTL on 3BS was detected in all three populations centered at the Fhb1 interval. The Rht-B1 locus governing plant height was found to have a strong effect in modulating FHB severity in all populations. The negative effect of the semi-dwarf allele Rht-B1b on FHB resistance was compensated by combining with Fhb1 and additional resistance QTL. The successful deployment of Fhb1 in T. durum was further substantiated by assessing type 2 resistance in one population. The efficient introgression of Fhb1 represents a significant step forward for enhancing FHB resistance in durum wheat. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/27662843/QTL_mapping_of_Fusarium_head_blight_resistance_in_three_related_durum_wheat_populations_ L2 - https://dx.doi.org/10.1007/s00122-016-2785-0 DB - PRIME DP - Unbound Medicine ER -