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A UHPLC method for the simultaneous analysis of biogenic amines, amino acids and ammonium ions in beer.
Food Chem. 2017 Feb 15; 217:117-124.FC

Abstract

This paper reports a novel UHPLC method for simultaneously quantifying nine biogenic amines, 21 amino acids, and ammonium ions, in beer. Precision values of standard curves slopes were lower than 3.4% and recovery was between 85% and 106%, indicating the absence of matrix effect. Linear calibration curves were obtained for analyte concentrations between two and four orders of magnitude (R(2)>0.996). Repeatability tests returned mean variations of 3.2% and 0.5% for beer and a standard solution, respectively. Sensitivity ranged between 0.03mg/L and 0.63mg/L for the biogenic amines, and 0.05mg/L and 5.19mg/L for other compounds. Original data on the habitual presence of ethanolamine in beers are presented. The method allows for more samples to be assayed per unit time, it uses less solvent than other techniques and therefore reduces costs and the associated waste. It could be a valuable tool for monitoring the safety and quality of beers.

Authors+Show Affiliations

Dairy Research Institute (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain. Electronic address: bredruel@ipla.csic.es.Dairy Research Institute (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain. Electronic address: ladero@ipla.csic.es.Dairy Research Institute (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain. Electronic address: beadelrio@ipla.csic.es.Dairy Research Institute (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain. Electronic address: mfernandez@ipla.csic.es.Dairy Research Institute (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain. Electronic address: mcm@ipla.csic.es.Dairy Research Institute (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain. Electronic address: maag@ipla.csic.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27664616

Citation

Redruello, Begoña, et al. "A UHPLC Method for the Simultaneous Analysis of Biogenic Amines, Amino Acids and Ammonium Ions in Beer." Food Chemistry, vol. 217, 2017, pp. 117-124.
Redruello B, Ladero V, Del Rio B, et al. A UHPLC method for the simultaneous analysis of biogenic amines, amino acids and ammonium ions in beer. Food Chem. 2017;217:117-124.
Redruello, B., Ladero, V., Del Rio, B., Fernández, M., Martin, M. C., & Alvarez, M. A. (2017). A UHPLC method for the simultaneous analysis of biogenic amines, amino acids and ammonium ions in beer. Food Chemistry, 217, 117-124. https://doi.org/10.1016/j.foodchem.2016.08.040
Redruello B, et al. A UHPLC Method for the Simultaneous Analysis of Biogenic Amines, Amino Acids and Ammonium Ions in Beer. Food Chem. 2017 Feb 15;217:117-124. PubMed PMID: 27664616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A UHPLC method for the simultaneous analysis of biogenic amines, amino acids and ammonium ions in beer. AU - Redruello,Begoña, AU - Ladero,Victor, AU - Del Rio,Beatriz, AU - Fernández,María, AU - Martin,M C, AU - Alvarez,Miguel A, Y1 - 2016/08/16/ PY - 2016/02/12/received PY - 2016/07/08/revised PY - 2016/08/13/accepted PY - 2016/9/25/entrez PY - 2016/9/25/pubmed PY - 2016/12/31/medline KW - Agmatine (PubChem CID: 199) KW - Amino acid KW - Beer KW - Biogenic amines KW - Cadaverine (PubChem CID: 273) KW - DEEMM KW - Diethylethoxymethylenemalonate (PubChem CID: 6871) KW - Ethanolamine (PubChem CID: 700) KW - Ethylamine (PubChem CID: 6341) KW - Histamine (PubChem CID: 774) KW - Putrescine (PubChem CID: 1045) KW - Tryptamine (PubChem CID: 1150) KW - Tyramine (PubChem CID: 5610) KW - UHPLC KW - β-Phenylethylamine (PubChem CID: 1001) SP - 117 EP - 124 JF - Food chemistry JO - Food Chem VL - 217 N2 - This paper reports a novel UHPLC method for simultaneously quantifying nine biogenic amines, 21 amino acids, and ammonium ions, in beer. Precision values of standard curves slopes were lower than 3.4% and recovery was between 85% and 106%, indicating the absence of matrix effect. Linear calibration curves were obtained for analyte concentrations between two and four orders of magnitude (R(2)>0.996). Repeatability tests returned mean variations of 3.2% and 0.5% for beer and a standard solution, respectively. Sensitivity ranged between 0.03mg/L and 0.63mg/L for the biogenic amines, and 0.05mg/L and 5.19mg/L for other compounds. Original data on the habitual presence of ethanolamine in beers are presented. The method allows for more samples to be assayed per unit time, it uses less solvent than other techniques and therefore reduces costs and the associated waste. It could be a valuable tool for monitoring the safety and quality of beers. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/27664616/A_UHPLC_method_for_the_simultaneous_analysis_of_biogenic_amines_amino_acids_and_ammonium_ions_in_beer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(16)31280-8 DB - PRIME DP - Unbound Medicine ER -