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Genetic variability and forensic efficiency of 39 microsatellite loci in the Li ethnic group from Hainan Island in the South China Sea.
Ann Hum Biol. 2017 Aug; 44(5):467-474.AH

Abstract

BACKGROUND

Investigation of allele and genotype frequencies of microsatellite loci in various populations is an essential pre-requisite in forensic application.

AIM

The present study obtained population genetic data and forensic parameters of 39 autosomal Short Tandem Repeats (STRs) loci from a Chinese Li ethnic group and estimated the genetic relationships between Li and other reference populations.

SUBJECTS AND METHODS

Thirty-nine STR loci, which include D19S433, D5S818, D21S11, D18S51, D6S1043, D3S1358, D13S317, D7S820, D16S539, CSF1PO, Penta D, D2S441, vWA, D8S1179, TPOX, Penta E, TH01, D12S391, D2S1338, FGA, D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, D1S1656, D22-GATA198B05, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D7S3048, D17S1290 and D5S2500, were amplified in two multiplex DNA-STR fluorescence detection systems for 189 unrelated healthy individuals of the Chinese Li ethnic group. The allele frequency distribution and several parameters commonly used in forensic science were statistically analysed.

RESULTS

A total of 378 alleles were observed with corresponding allelic frequencies ranging from 0.0026-0.5899. The power of discrimination and power of exclusion ranged from 0.7569-0.9672 and 0.2513-0.7355, respectively. The power of exclusion (PE) ranged from 0.2580-0.7943 for trio paternity cases and 0.1693-0.5940 for duo paternity cases. The polymorphism information content (PIC) ranged from 0.5001-0.8611. The cumulative match probability across these 39 loci was 2.4242 × 10-38.

CONCLUSION

The results indicate that 39 STR loci are polymorphic among the Li ethnic group in Hainan Island in the South China Sea. This set of polymorphic STR loci provide highly polymorphic information and forensic efficiency for forensic individual identification and paternity testing, as well as basic population data for population genetics and anthropological research.

Authors+Show Affiliations

a CAS Key Laboratory of Genome Sciences and Information , Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing , PR China.a CAS Key Laboratory of Genome Sciences and Information , Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing , PR China.a CAS Key Laboratory of Genome Sciences and Information , Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing , PR China.a CAS Key Laboratory of Genome Sciences and Information , Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing , PR China.b Guangzhou Forensic Science Institute , Guangdong , PR China.c Beijing Microread Genetics Co., Ltd , Beijing , PR China.c Beijing Microread Genetics Co., Ltd , Beijing , PR China.d Beijing Center for Physical and Chemical Analysis , Beijing , PR China. e Beijing Engineering Technology Research Center of Gene Sequencing and Gene Function Analysis , Beijing , PR China.a CAS Key Laboratory of Genome Sciences and Information , Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing , PR China.d Beijing Center for Physical and Chemical Analysis , Beijing , PR China. e Beijing Engineering Technology Research Center of Gene Sequencing and Gene Function Analysis , Beijing , PR China.a CAS Key Laboratory of Genome Sciences and Information , Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing , PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27670224

Citation

Chen, Jing, et al. "Genetic Variability and Forensic Efficiency of 39 Microsatellite Loci in the Li Ethnic Group From Hainan Island in the South China Sea." Annals of Human Biology, vol. 44, no. 5, 2017, pp. 467-474.
Chen J, Xie B, Yang Y, et al. Genetic variability and forensic efficiency of 39 microsatellite loci in the Li ethnic group from Hainan Island in the South China Sea. Ann Hum Biol. 2017;44(5):467-474.
Chen, J., Xie, B., Yang, Y., Yang, M., Liu, C., Lv, Y., Chen, C., Liu, X., Fang, X., Wu, H., & Yan, J. (2017). Genetic variability and forensic efficiency of 39 microsatellite loci in the Li ethnic group from Hainan Island in the South China Sea. Annals of Human Biology, 44(5), 467-474. https://doi.org/10.1080/03014460.2016.1241300
Chen J, et al. Genetic Variability and Forensic Efficiency of 39 Microsatellite Loci in the Li Ethnic Group From Hainan Island in the South China Sea. Ann Hum Biol. 2017;44(5):467-474. PubMed PMID: 27670224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic variability and forensic efficiency of 39 microsatellite loci in the Li ethnic group from Hainan Island in the South China Sea. AU - Chen,Jing, AU - Xie,Bingbing, AU - Yang,Yaran, AU - Yang,Meng, AU - Liu,Chao, AU - Lv,Yuexin, AU - Chen,Chuguang, AU - Liu,Xu, AU - Fang,Xiangdong, AU - Wu,Huijuan, AU - Yan,Jiangwei, Y1 - 2016/11/13/ PY - 2016/9/28/pubmed PY - 2018/4/7/medline PY - 2016/9/28/entrez KW - Chinese Li group KW - Short tandem repeats KW - forensic genetics KW - genetic polymorphisms KW - inter-population comparison SP - 467 EP - 474 JF - Annals of human biology JO - Ann Hum Biol VL - 44 IS - 5 N2 - BACKGROUND: Investigation of allele and genotype frequencies of microsatellite loci in various populations is an essential pre-requisite in forensic application. AIM: The present study obtained population genetic data and forensic parameters of 39 autosomal Short Tandem Repeats (STRs) loci from a Chinese Li ethnic group and estimated the genetic relationships between Li and other reference populations. SUBJECTS AND METHODS: Thirty-nine STR loci, which include D19S433, D5S818, D21S11, D18S51, D6S1043, D3S1358, D13S317, D7S820, D16S539, CSF1PO, Penta D, D2S441, vWA, D8S1179, TPOX, Penta E, TH01, D12S391, D2S1338, FGA, D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, D1S1656, D22-GATA198B05, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D7S3048, D17S1290 and D5S2500, were amplified in two multiplex DNA-STR fluorescence detection systems for 189 unrelated healthy individuals of the Chinese Li ethnic group. The allele frequency distribution and several parameters commonly used in forensic science were statistically analysed. RESULTS: A total of 378 alleles were observed with corresponding allelic frequencies ranging from 0.0026-0.5899. The power of discrimination and power of exclusion ranged from 0.7569-0.9672 and 0.2513-0.7355, respectively. The power of exclusion (PE) ranged from 0.2580-0.7943 for trio paternity cases and 0.1693-0.5940 for duo paternity cases. The polymorphism information content (PIC) ranged from 0.5001-0.8611. The cumulative match probability across these 39 loci was 2.4242 × 10-38. CONCLUSION: The results indicate that 39 STR loci are polymorphic among the Li ethnic group in Hainan Island in the South China Sea. This set of polymorphic STR loci provide highly polymorphic information and forensic efficiency for forensic individual identification and paternity testing, as well as basic population data for population genetics and anthropological research. SN - 1464-5033 UR - https://www.unboundmedicine.com/medline/citation/27670224/Genetic_variability_and_forensic_efficiency_of_39_microsatellite_loci_in_the_Li_ethnic_group_from_Hainan_Island_in_the_South_China_Sea_ DB - PRIME DP - Unbound Medicine ER -