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Simultaneous quantification of triterpenoid saponins in rat plasma by UHPLC-MS/MS and its application to a pharmacokinetic study after oral total saponin of Aralia elata leaves.
J Sep Sci. 2016 Nov; 39(22):4360-4368.JS

Abstract

A rapid, sensitive, and reliable analytical ultra performance liquid chromatography with tandem mass spectrometry method was developed for the simultaneous determination of Aralia-saponin IV, 3-O-β-d-glucopyranosyl-(1→3)-β-d-glucopyranosyl-(1→3)-β-d-glucopyranosyl oleanolic acid 28-O-β-d-glucopyranoside, Aralia-saponin A and Aralia-saponin B after the oral administration of total saponin of Aralia elata leaves in rat plasma. Plasma samples were pretreated by protein precipitation with methanol. The analysis was performed on an ACQUITY UPLC HSS T3 column. The detection was performed on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode using an electrospray ionization source with negative ionization mode. Under the experimental conditions, the calibration curves of four analytes had good linearity values (r > 0.991). The intra- and inter-day precision values of the four analytes were ≤ 11.6%, and the accuracy was between -6.2 and 4.2%.The extraction recoveries of four triterpenoid saponins were in the range of 84.06-91.66% (RSD < 10.5%), and all values of the matrix effect were more than 90.30%. The developed analytical method was successfully applied to pharmacokinetic study on simultaneous determination of the four triterpenoid saponins in rat plasma after oral administration of total saponin of Aralia elata leaves, which helps guiding clinical usage of Aralia elata leaves.

Authors+Show Affiliations

Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, China.Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, China.Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, China.Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, China.Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, China.Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, China.Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, China.Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, China.Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27670645

Citation

Sun, Yichun, et al. "Simultaneous Quantification of Triterpenoid Saponins in Rat Plasma By UHPLC-MS/MS and Its Application to a Pharmacokinetic Study After Oral Total Saponin of Aralia Elata Leaves." Journal of Separation Science, vol. 39, no. 22, 2016, pp. 4360-4368.
Sun Y, Xue J, Li B, et al. Simultaneous quantification of triterpenoid saponins in rat plasma by UHPLC-MS/MS and its application to a pharmacokinetic study after oral total saponin of Aralia elata leaves. J Sep Sci. 2016;39(22):4360-4368.
Sun, Y., Xue, J., Li, B., Lin, X., Wang, Z., Jiang, H., Zhang, H., Wang, Q., & Kuang, H. (2016). Simultaneous quantification of triterpenoid saponins in rat plasma by UHPLC-MS/MS and its application to a pharmacokinetic study after oral total saponin of Aralia elata leaves. Journal of Separation Science, 39(22), 4360-4368. https://doi.org/10.1002/jssc.201600801
Sun Y, et al. Simultaneous Quantification of Triterpenoid Saponins in Rat Plasma By UHPLC-MS/MS and Its Application to a Pharmacokinetic Study After Oral Total Saponin of Aralia Elata Leaves. J Sep Sci. 2016;39(22):4360-4368. PubMed PMID: 27670645.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantification of triterpenoid saponins in rat plasma by UHPLC-MS/MS and its application to a pharmacokinetic study after oral total saponin of Aralia elata leaves. AU - Sun,Yichun, AU - Xue,Juan, AU - Li,Baimei, AU - Lin,Xiaoting, AU - Wang,Zhibin, AU - Jiang,Hai, AU - Zhang,Hongwei, AU - Wang,Qiuhong, AU - Kuang,Haixue, Y1 - 2016/10/28/ PY - 2016/07/19/received PY - 2016/08/23/revised PY - 2016/09/16/accepted PY - 2016/10/30/pubmed PY - 2018/3/7/medline PY - 2016/9/28/entrez KW - Aralia elata leaves KW - Pharmacokinetics KW - Tandem mass spectrometry KW - Triterpenoid saponins SP - 4360 EP - 4368 JF - Journal of separation science JO - J Sep Sci VL - 39 IS - 22 N2 - A rapid, sensitive, and reliable analytical ultra performance liquid chromatography with tandem mass spectrometry method was developed for the simultaneous determination of Aralia-saponin IV, 3-O-β-d-glucopyranosyl-(1→3)-β-d-glucopyranosyl-(1→3)-β-d-glucopyranosyl oleanolic acid 28-O-β-d-glucopyranoside, Aralia-saponin A and Aralia-saponin B after the oral administration of total saponin of Aralia elata leaves in rat plasma. Plasma samples were pretreated by protein precipitation with methanol. The analysis was performed on an ACQUITY UPLC HSS T3 column. The detection was performed on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode using an electrospray ionization source with negative ionization mode. Under the experimental conditions, the calibration curves of four analytes had good linearity values (r > 0.991). The intra- and inter-day precision values of the four analytes were ≤ 11.6%, and the accuracy was between -6.2 and 4.2%.The extraction recoveries of four triterpenoid saponins were in the range of 84.06-91.66% (RSD < 10.5%), and all values of the matrix effect were more than 90.30%. The developed analytical method was successfully applied to pharmacokinetic study on simultaneous determination of the four triterpenoid saponins in rat plasma after oral administration of total saponin of Aralia elata leaves, which helps guiding clinical usage of Aralia elata leaves. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/27670645/Simultaneous_quantification_of_triterpenoid_saponins_in_rat_plasma_by_UHPLC_MS/MS_and_its_application_to_a_pharmacokinetic_study_after_oral_total_saponin_of_Aralia_elata_leaves_ L2 - https://doi.org/10.1002/jssc.201600801 DB - PRIME DP - Unbound Medicine ER -