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Symplocos cochinchinensis enhances insulin sensitivity via the down regulation of lipogenesis and insulin resistance in high energy diet rat model.
J Ethnopharmacol. 2016 Dec 04; 193:500-509.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

This plant has been utilized in Indian system of medicine for treatment of diabetes. This is clearly evident from the composition of Ayurvedic preparation for diabetes 'Nisakathakadi Kashayam' where this is one of the main ingredients of this preparation AIM OF THE STUDY: The study aims in elucidating the molecular mechanisms underlying the insulin sensitizing effects of Symplocos cochinchinensis ethanol extract (SCE) using a high fructose and saturated fat (HFS) fed insulin resistant rat model.

MATERIALS AND METHODS

Experimental groups consisted of normal diet (ND), ND+SCE 500mg/kg bwd, HFS+vehicle, HFS+metformin 100mg/kg bwd, HFS+SCE 250/500mg/kg bwd. Initially the animals were kept under HFS diet for 8 weeks, and at the end of 8 week period, animals were found to develop insulin resistance and dyslipidemia. Post-administration of SCE, metformin or vehicle were carried out for 3 weeks. Gene and protein expressions relevant to insulin signalling pathway were analysed.

RESULTS

HFS significantly altered the normal physiology of animals via proteins and genes relevant to metabolism like stearoyl-CoA desaturase (SCD1), sterol regulatory element binding protein 1 (SREBP-1c), fatty acid synthase (FAS), glucose 6 phosphatase (G6Pase), phosphoenol pyruvate carboxykinase (PEPCK), glucose transporter 2 (GLUT2), protein tyrosine phosphatse 1B (PTP1B), peroxisome proliferator activated receptor alpha (PPAR alpha), sirtuin 1 (SIRT1) and glucokinase. SCE administration attenuates the insulin resistance in HFS rat by the down regulation of SCD1 gene expression that modulates SREBP-1c dependent and independent hepatic lipid accumulation.

CONCLUSION

SCE enhances insulin sensitivity via the down regulation of lipogenesis and insulin resistance in HFS rat model.

Authors+Show Affiliations

Agroprocessing and Natural Products Division, CSIR - National Institute for Interdisciplinary Science and Technology(NIIST), Thiruvananthapuram 695019, Kerala, India.Agroprocessing and Natural Products Division, CSIR - National Institute for Interdisciplinary Science and Technology(NIIST), Thiruvananthapuram 695019, Kerala, India.Agroprocessing and Natural Products Division, CSIR - National Institute for Interdisciplinary Science and Technology(NIIST), Thiruvananthapuram 695019, Kerala, India.Division of Biochemistry, CSIR - Central Drug Research Institute(CDRI), Sector 10, Janakipuram Extension, Sitapur Road, Lucknow 226031, Uttar Pradesh, India.Division of Biochemistry, CSIR - Central Drug Research Institute(CDRI), Sector 10, Janakipuram Extension, Sitapur Road, Lucknow 226031, Uttar Pradesh, India.Agroprocessing and Natural Products Division, CSIR - National Institute for Interdisciplinary Science and Technology(NIIST), Thiruvananthapuram 695019, Kerala, India. Electronic address: raghukgopal@niist.res.in.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

27686268

Citation

Antu, Kalathookunnel Antony, et al. "Symplocos Cochinchinensis Enhances Insulin Sensitivity Via the Down Regulation of Lipogenesis and Insulin Resistance in High Energy Diet Rat Model." Journal of Ethnopharmacology, vol. 193, 2016, pp. 500-509.
Antu KA, Riya MP, Nair A, et al. Symplocos cochinchinensis enhances insulin sensitivity via the down regulation of lipogenesis and insulin resistance in high energy diet rat model. J Ethnopharmacol. 2016;193:500-509.
Antu, K. A., Riya, M. P., Nair, A., Mishra, A., Srivastava, A. K., & Raghu, K. G. (2016). Symplocos cochinchinensis enhances insulin sensitivity via the down regulation of lipogenesis and insulin resistance in high energy diet rat model. Journal of Ethnopharmacology, 193, 500-509. https://doi.org/10.1016/j.jep.2016.09.050
Antu KA, et al. Symplocos Cochinchinensis Enhances Insulin Sensitivity Via the Down Regulation of Lipogenesis and Insulin Resistance in High Energy Diet Rat Model. J Ethnopharmacol. 2016 Dec 4;193:500-509. PubMed PMID: 27686268.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Symplocos cochinchinensis enhances insulin sensitivity via the down regulation of lipogenesis and insulin resistance in high energy diet rat model. AU - Antu,Kalathookunnel Antony, AU - Riya,Mariam Philip, AU - Nair,Anupama, AU - Mishra,Arvind, AU - Srivastava,Arvind K, AU - Raghu,Kozhiparambil Gopalan, Y1 - 2016/09/27/ PY - 2016/03/22/received PY - 2016/09/25/revised PY - 2016/09/25/accepted PY - 2016/10/1/pubmed PY - 2017/4/18/medline PY - 2016/10/1/entrez KW - Chloroform (PubChem CID: 6212) KW - Diabetes KW - Ethanol (PubChem CID: 702) KW - Glucose (PubChem CID: 5793) KW - Glycine (PubChem CID: 750) KW - Hepatic steatosis KW - Insulin resistance KW - Metformin (PubChem CID: 14219) KW - Methanol (PubChem CID: 887) KW - SCD1 KW - SDS (PubChem CID: 3423265) KW - SIRT1 KW - Sodium chloride (PubChem CID: 5234) KW - Symplocos cochinchinensis SP - 500 EP - 509 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 193 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: This plant has been utilized in Indian system of medicine for treatment of diabetes. This is clearly evident from the composition of Ayurvedic preparation for diabetes 'Nisakathakadi Kashayam' where this is one of the main ingredients of this preparation AIM OF THE STUDY: The study aims in elucidating the molecular mechanisms underlying the insulin sensitizing effects of Symplocos cochinchinensis ethanol extract (SCE) using a high fructose and saturated fat (HFS) fed insulin resistant rat model. MATERIALS AND METHODS: Experimental groups consisted of normal diet (ND), ND+SCE 500mg/kg bwd, HFS+vehicle, HFS+metformin 100mg/kg bwd, HFS+SCE 250/500mg/kg bwd. Initially the animals were kept under HFS diet for 8 weeks, and at the end of 8 week period, animals were found to develop insulin resistance and dyslipidemia. Post-administration of SCE, metformin or vehicle were carried out for 3 weeks. Gene and protein expressions relevant to insulin signalling pathway were analysed. RESULTS: HFS significantly altered the normal physiology of animals via proteins and genes relevant to metabolism like stearoyl-CoA desaturase (SCD1), sterol regulatory element binding protein 1 (SREBP-1c), fatty acid synthase (FAS), glucose 6 phosphatase (G6Pase), phosphoenol pyruvate carboxykinase (PEPCK), glucose transporter 2 (GLUT2), protein tyrosine phosphatse 1B (PTP1B), peroxisome proliferator activated receptor alpha (PPAR alpha), sirtuin 1 (SIRT1) and glucokinase. SCE administration attenuates the insulin resistance in HFS rat by the down regulation of SCD1 gene expression that modulates SREBP-1c dependent and independent hepatic lipid accumulation. CONCLUSION: SCE enhances insulin sensitivity via the down regulation of lipogenesis and insulin resistance in HFS rat model. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/27686268/Symplocos_cochinchinensis_enhances_insulin_sensitivity_via_the_down_regulation_of_lipogenesis_and_insulin_resistance_in_high_energy_diet_rat_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)31005-4 DB - PRIME DP - Unbound Medicine ER -