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Clinical spectrum, diagnostic criteria, and polymerase chain reaction of aqueous humor in viral and toxoplasma detection in Fuchs' uveitis syndrome.
Indian J Ophthalmol. 2016 Aug; 64(8):555-8.IJ

Abstract

AIM

The aim of this study is to describe the clinical features and diagnostic criteria of Fuchs' uveitis (FU) and to determine whether it has an association with virus and toxoplasma in the aqueous humor during cataract surgery.

SETTING AND DESIGN

This is a prospective, case-control study.

MATERIALS AND METHODS

Patients with FU (n = 25), anterior uveitis (n = 15), and no uveitis (normal) (n = 50) were included based on predefined inclusion and exclusion criteria for all three groups. Polymerase chain reaction (PCR) of aqueous humor and serum for rubella, herpes simplex virus (HSV), cytomegalovirus (CMV), varicella-zoster virus (VZV), and toxoplasma was done using conventional uniplex PCR.

STATISTICAL ANALYSIS

It was done using SPSS software using Chi-square test for categorical variables, and P < 0.05 was considered statistically significant.

RESULTS

Ninety patients were enrolled in the study in three groups, comparable for age, gender, and laterality of ocular involvement. All patients had diffuse keratic precipitates in FU group (P = 0001) with none having posterior synechiae (P = 0.046) which was statistically significant when compared to anterior uveitis patients. Iris nodules were noted in one case in both groups. Serum and aqueous PCR was negative for detection of VZV, CMV, toxoplasma, and rubella in all groups. PCR for HSV was positive in one patient in "normal" group but was not statistically significant.

CONCLUSION

Our study shows that diagnosis of FU is mainly clinical. There appears to be no role of aqueous humor testing for viruses by PCR to aid in etiological diagnosis.

Authors+Show Affiliations

Department of Cornea and Anterior Segment, Tej Kohli Cornea Institute, L.V. Prasad Eye Institute, Hyderabad, Telangana, India.Department of Cornea and Anterior Segment, Tej Kohli Cornea Institute, L.V. Prasad Eye Institute, Hyderabad, Telangana, India.Jhaveri Microbiology Center, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.Center for Ocular Regeneration, Srujana Innovation Center, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.Department of Clinical Research, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.Jhaveri Microbiology Center, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27688274

Citation

Sabhapandit, Swapnali, et al. "Clinical Spectrum, Diagnostic Criteria, and Polymerase Chain Reaction of Aqueous Humor in Viral and Toxoplasma Detection in Fuchs' Uveitis Syndrome." Indian Journal of Ophthalmology, vol. 64, no. 8, 2016, pp. 555-8.
Sabhapandit S, Murthy SI, Balne PK, et al. Clinical spectrum, diagnostic criteria, and polymerase chain reaction of aqueous humor in viral and toxoplasma detection in Fuchs' uveitis syndrome. Indian J Ophthalmol. 2016;64(8):555-8.
Sabhapandit, S., Murthy, S. I., Balne, P. K., Sangwan, V. S., Sumanth, V., & Reddy, A. K. (2016). Clinical spectrum, diagnostic criteria, and polymerase chain reaction of aqueous humor in viral and toxoplasma detection in Fuchs' uveitis syndrome. Indian Journal of Ophthalmology, 64(8), 555-8. https://doi.org/10.4103/0301-4738.191485
Sabhapandit S, et al. Clinical Spectrum, Diagnostic Criteria, and Polymerase Chain Reaction of Aqueous Humor in Viral and Toxoplasma Detection in Fuchs' Uveitis Syndrome. Indian J Ophthalmol. 2016;64(8):555-8. PubMed PMID: 27688274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clinical spectrum, diagnostic criteria, and polymerase chain reaction of aqueous humor in viral and toxoplasma detection in Fuchs' uveitis syndrome. AU - Sabhapandit,Swapnali, AU - Murthy,Somasheila I, AU - Balne,Praveen K, AU - Sangwan,Virender Singh, AU - Sumanth,V, AU - Reddy,Ashok K, PY - 2016/10/1/entrez PY - 2016/10/1/pubmed PY - 2017/7/8/medline SP - 555 EP - 8 JF - Indian journal of ophthalmology JO - Indian J Ophthalmol VL - 64 IS - 8 N2 - AIM: The aim of this study is to describe the clinical features and diagnostic criteria of Fuchs' uveitis (FU) and to determine whether it has an association with virus and toxoplasma in the aqueous humor during cataract surgery. SETTING AND DESIGN: This is a prospective, case-control study. MATERIALS AND METHODS: Patients with FU (n = 25), anterior uveitis (n = 15), and no uveitis (normal) (n = 50) were included based on predefined inclusion and exclusion criteria for all three groups. Polymerase chain reaction (PCR) of aqueous humor and serum for rubella, herpes simplex virus (HSV), cytomegalovirus (CMV), varicella-zoster virus (VZV), and toxoplasma was done using conventional uniplex PCR. STATISTICAL ANALYSIS: It was done using SPSS software using Chi-square test for categorical variables, and P < 0.05 was considered statistically significant. RESULTS: Ninety patients were enrolled in the study in three groups, comparable for age, gender, and laterality of ocular involvement. All patients had diffuse keratic precipitates in FU group (P = 0001) with none having posterior synechiae (P = 0.046) which was statistically significant when compared to anterior uveitis patients. Iris nodules were noted in one case in both groups. Serum and aqueous PCR was negative for detection of VZV, CMV, toxoplasma, and rubella in all groups. PCR for HSV was positive in one patient in "normal" group but was not statistically significant. CONCLUSION: Our study shows that diagnosis of FU is mainly clinical. There appears to be no role of aqueous humor testing for viruses by PCR to aid in etiological diagnosis. SN - 1998-3689 UR - https://www.unboundmedicine.com/medline/citation/27688274/Clinical_spectrum_diagnostic_criteria_and_polymerase_chain_reaction_of_aqueous_humor_in_viral_and_toxoplasma_detection_in_Fuchs'_uveitis_syndrome_ L2 - http://www.ijo.in/article.asp?issn=0301-4738;year=2016;volume=64;issue=8;spage=555;epage=558;aulast=Sabhapandit DB - PRIME DP - Unbound Medicine ER -