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Economical, Plain, and Rapid Authentication of Actaea racemosa L. (syn. Cimicifuga racemosa, Black Cohosh) Herbal Raw Material by Resilient RP-PDA-HPLC and Chemometric Analysis.
Phytochem Anal. 2016 Nov; 27(6):318-325.PA

Abstract

INTRODUCTION

The medicinal plant Actaea racemosa L. (Ranunculaceae, aka black cohosh) is widely used to treat climacteric complaints as an alternative to hormone substitution. Recent trials prove efficacy and safety of the approved herbal medicinal products from extracts of pharmaceutical quality. This led to worldwide increasing sales. A higher demand for the plant material results in problems with economically motivated adulteration. Thus, reliable tools for herbal drug authentication are necessary.

OBJECTIVE

To develop an economical, plain, and rapid method to distinguish between closely related American and Asian Actaea species, using securely established and resilient analytical methods coupled to a chemometric evaluation of the resulting data.

METHODOLOGY

We developed and validated a RP-PDA-HPLC method including an extraction by ultra-sonication to determine the genuine contents of partly hydrolysis-sensitive polyphenols in Actaea racemosa roots and rhizomes, and applied it to a large number of 203 Actaea samples consisting of seven species.

RESULTS

We were able to generate reliable data with regards to the polyphenolic esters in the samples. The evaluation of this data by principle component analysis (PCA) made a discrimination between Asian Actaea species (sheng ma), one American Actaea species (Appalachian bugbane), and A. racemosa possible.

CONCLUSION

The developed RP-PDA-HPLC method coupled to PCA is an excellent tool for authentication of the Actaea racemosa herbal drug, and can be a powerful addition to the TLC methods used in the dedicated pharmacopoeias, and is a promising alternative to expensive and lots of expertise requiring methods. Copyright © 2016 John Wiley & Sons, Ltd.

Authors+Show Affiliations

Institute of Pharmacy, Freie Universität Berlin, Königin-Luise-Str. 2+4, 14195, Berlin, Germany.Albrecht Daniel Thaer-Institut für Agrar- und Gartenbauwissenschaften, Humboldt-Universität zu Berlin, Albrecht-Thaer-Weg 5, 14195, Berlin, Germany.Institute of Chemistry and Biochemistry, Core Facility BioSupraMol, Freie Universität Berlin, Takustr. 3, 14195, Berlin, Germany.Institute of Pharmacy, Freie Universität Berlin, Königin-Luise-Str. 2+4, 14195, Berlin, Germany. melzig@zedat.fu-berlin.de.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

27690371

Citation

Bittner, Marian, et al. "Economical, Plain, and Rapid Authentication of Actaea Racemosa L. (syn. Cimicifuga Racemosa, Black Cohosh) Herbal Raw Material By Resilient RP-PDA-HPLC and Chemometric Analysis." Phytochemical Analysis : PCA, vol. 27, no. 6, 2016, pp. 318-325.
Bittner M, Schenk R, Springer A, et al. Economical, Plain, and Rapid Authentication of Actaea racemosa L. (syn. Cimicifuga racemosa, Black Cohosh) Herbal Raw Material by Resilient RP-PDA-HPLC and Chemometric Analysis. Phytochem Anal. 2016;27(6):318-325.
Bittner, M., Schenk, R., Springer, A., & Melzig, M. F. (2016). Economical, Plain, and Rapid Authentication of Actaea racemosa L. (syn. Cimicifuga racemosa, Black Cohosh) Herbal Raw Material by Resilient RP-PDA-HPLC and Chemometric Analysis. Phytochemical Analysis : PCA, 27(6), 318-325. https://doi.org/10.1002/pca.2622
Bittner M, et al. Economical, Plain, and Rapid Authentication of Actaea Racemosa L. (syn. Cimicifuga Racemosa, Black Cohosh) Herbal Raw Material By Resilient RP-PDA-HPLC and Chemometric Analysis. Phytochem Anal. 2016;27(6):318-325. PubMed PMID: 27690371.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Economical, Plain, and Rapid Authentication of Actaea racemosa L. (syn. Cimicifuga racemosa, Black Cohosh) Herbal Raw Material by Resilient RP-PDA-HPLC and Chemometric Analysis. AU - Bittner,Marian, AU - Schenk,Regina, AU - Springer,Andreas, AU - Melzig,Matthias F, Y1 - 2016/09/30/ PY - 2015/12/09/received PY - 2016/03/21/revised PY - 2016/03/25/accepted PY - 2016/10/22/pubmed PY - 2017/2/28/medline PY - 2016/10/1/entrez KW - actaea racemosa KW - black cohosh KW - cimicifuga racemosa, sheng ma, 升麻 SP - 318 EP - 325 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 27 IS - 6 N2 - INTRODUCTION: The medicinal plant Actaea racemosa L. (Ranunculaceae, aka black cohosh) is widely used to treat climacteric complaints as an alternative to hormone substitution. Recent trials prove efficacy and safety of the approved herbal medicinal products from extracts of pharmaceutical quality. This led to worldwide increasing sales. A higher demand for the plant material results in problems with economically motivated adulteration. Thus, reliable tools for herbal drug authentication are necessary. OBJECTIVE: To develop an economical, plain, and rapid method to distinguish between closely related American and Asian Actaea species, using securely established and resilient analytical methods coupled to a chemometric evaluation of the resulting data. METHODOLOGY: We developed and validated a RP-PDA-HPLC method including an extraction by ultra-sonication to determine the genuine contents of partly hydrolysis-sensitive polyphenols in Actaea racemosa roots and rhizomes, and applied it to a large number of 203 Actaea samples consisting of seven species. RESULTS: We were able to generate reliable data with regards to the polyphenolic esters in the samples. The evaluation of this data by principle component analysis (PCA) made a discrimination between Asian Actaea species (sheng ma), one American Actaea species (Appalachian bugbane), and A. racemosa possible. CONCLUSION: The developed RP-PDA-HPLC method coupled to PCA is an excellent tool for authentication of the Actaea racemosa herbal drug, and can be a powerful addition to the TLC methods used in the dedicated pharmacopoeias, and is a promising alternative to expensive and lots of expertise requiring methods. Copyright © 2016 John Wiley & Sons, Ltd. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/27690371/Economical_Plain_and_Rapid_Authentication_of_Actaea_racemosa_L___syn__Cimicifuga_racemosa_Black_Cohosh__Herbal_Raw_Material_by_Resilient_RP_PDA_HPLC_and_Chemometric_Analysis_ L2 - https://doi.org/10.1002/pca.2622 DB - PRIME DP - Unbound Medicine ER -