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Synthesis and characterization of chitosan-TiO2:Cu nanocomposite and their enhanced antimicrobial activity with visible light.
Colloids Surf B Biointerfaces. 2016 Dec 01; 148:566-575.CS

Abstract

In the present investigation, novel strategy for the preparation of hybrid nanocomposite containing organic polymer (Chitosan) and inorganic (TiO2:Cu) nanoparticles (NPs) has been developed and demonstrated its biomedical application. The sol-gel and ultra-sonication method assisted for the preparation of uniformly distributed Chitosan-TiO2:Cu (CS-CT) nanocomposite. The structural properties of prepared CS-CT nanocomposite were studied by XRD and FTIR techniques. The XPS was used to estimate elemental composition of the nanocomposite. Thermal properties were studied using TGA. TEM and SEM analysis showed the non-spherical nature of NPs with the average mean diameter 16nm. The optical properties were analyzed with UV-vis diffuse reflectance spectroscopy to confirm optical absorption in the visible region of light. Where CS-CT showed 200% enhanced light mediated photocatalytic antimicrobial activity against microorganism (Escherichia coli and Staphylococcus aureus) as compared with control. The antimicrobial activity of CS-CT nanocomposite in presence of light is found to be enhanced than that of its components, this is due to synergistic effect of organic and inorganic material complimenting each other's activity. The OH radicals release studied by PL spectroscopy on the surface of nanocomposite was used to examine antibacterial activity. Cytotoxicity assessment of CS-CT on human fibroblast cells was performed by MTT assay.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Raut AV
Center for Interdisciplinary Research, D. Y. Patil University, Kolhapur 416006, M.S., India.
Yadav HM
Department of Materials Science & Engineering, University of Seoul, Seoul 02504, South Korea.
Gnanamani A
Microbiology Division, CSIR-CLRI, Adyar, Chennai 600036, TN, India.
Pushpavanam S
Department of Chemical Engineering, Indian Institute of Technology Madras (IIT Madras), Chennai 600036, TN, India.
Pawar SH
Center for Interdisciplinary Research, D. Y. Patil University, Kolhapur 416006, M.S., India. Electronic address: shpawar1946@gmail.com.

MeSH

AnimalsAnti-Bacterial AgentsCell SurvivalCells, CulturedChitosanCopperEscherichia coliHumansLightMetal NanoparticlesMiceMicroscopy, Electron, ScanningMicroscopy, Electron, TransmissionNIH 3T3 CellsNanocompositesPhotoelectron SpectroscopySpectroscopy, Fourier Transform InfraredStaphylococcus aureusTemperatureTitaniumX-Ray Diffraction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27693718

Citation

Raut, A V., et al. "Synthesis and Characterization of chitosan-TiO2:Cu Nanocomposite and Their Enhanced Antimicrobial Activity With Visible Light." Colloids and Surfaces. B, Biointerfaces, vol. 148, 2016, pp. 566-575.
Raut AV, Yadav HM, Gnanamani A, et al. Synthesis and characterization of chitosan-TiO2:Cu nanocomposite and their enhanced antimicrobial activity with visible light. Colloids Surf B Biointerfaces. 2016;148:566-575.
Raut, A. V., Yadav, H. M., Gnanamani, A., Pushpavanam, S., & Pawar, S. H. (2016). Synthesis and characterization of chitosan-TiO2:Cu nanocomposite and their enhanced antimicrobial activity with visible light. Colloids and Surfaces. B, Biointerfaces, 148, 566-575. https://doi.org/10.1016/j.colsurfb.2016.09.028
Raut AV, et al. Synthesis and Characterization of chitosan-TiO2:Cu Nanocomposite and Their Enhanced Antimicrobial Activity With Visible Light. Colloids Surf B Biointerfaces. 2016 Dec 1;148:566-575. PubMed PMID: 27693718.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis and characterization of chitosan-TiO2:Cu nanocomposite and their enhanced antimicrobial activity with visible light. AU - Raut,A V, AU - Yadav,H M, AU - Gnanamani,A, AU - Pushpavanam,S, AU - Pawar,S H, Y1 - 2016/09/22/ PY - 2016/06/08/received PY - 2016/09/05/revised PY - 2016/09/21/accepted PY - 2016/11/5/pubmed PY - 2017/3/11/medline PY - 2016/10/4/entrez KW - Biocompatible KW - CS-CT nanocomposite KW - Cell disruption KW - Hydroxyl radicle KW - Mechanisms of microbial adhesion KW - TiO(2):Cu KW - Visible light antimicrobial activity SP - 566 EP - 575 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 148 N2 - In the present investigation, novel strategy for the preparation of hybrid nanocomposite containing organic polymer (Chitosan) and inorganic (TiO2:Cu) nanoparticles (NPs) has been developed and demonstrated its biomedical application. The sol-gel and ultra-sonication method assisted for the preparation of uniformly distributed Chitosan-TiO2:Cu (CS-CT) nanocomposite. The structural properties of prepared CS-CT nanocomposite were studied by XRD and FTIR techniques. The XPS was used to estimate elemental composition of the nanocomposite. Thermal properties were studied using TGA. TEM and SEM analysis showed the non-spherical nature of NPs with the average mean diameter 16nm. The optical properties were analyzed with UV-vis diffuse reflectance spectroscopy to confirm optical absorption in the visible region of light. Where CS-CT showed 200% enhanced light mediated photocatalytic antimicrobial activity against microorganism (Escherichia coli and Staphylococcus aureus) as compared with control. The antimicrobial activity of CS-CT nanocomposite in presence of light is found to be enhanced than that of its components, this is due to synergistic effect of organic and inorganic material complimenting each other's activity. The OH radicals release studied by PL spectroscopy on the surface of nanocomposite was used to examine antibacterial activity. Cytotoxicity assessment of CS-CT on human fibroblast cells was performed by MTT assay. SN - 1873-4367 UR - https://www.unboundmedicine.com/medline/citation/27693718/Synthesis_and_characterization_of_chitosan_TiO2:Cu_nanocomposite_and_their_enhanced_antimicrobial_activity_with_visible_light_ DB - PRIME DP - Unbound Medicine ER -