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Simultaneous quantification and identification of flavonoids, lignans, coumarin and amides in leaves of Zanthoxylum armatum using UPLC-DAD-ESI-QTOF-MS/MS.
J Pharm Biomed Anal. 2017 Jan 05; 132:46-55.JP

Abstract

The current study presents isolation and characterization of twelve compounds including catechin (1), isovitexin (2), hesperidin (3), psoralin (4), eudesmin (5), kobusin (6), fargesin (7), sesamin (8), asarinin (9), planispine-A (10), α-sanshool (11) and vitexin (12), from the leaves of Zanthoxylum armatum. Further, two rapid and simple ultra performance liquid chromatography-diode array detection (UPLC-DAD) methods were developed for the simultaneous quantitative determination of isolated compounds from Z. armatum leaves. These analytical methods were validated for linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The LOD and LOQ were in the range of 0.06-0.21μg/mL and 0.19-0.69μg/mL, respectively. The validated method was linear (R2≥0.9906), precise in terms of peak area (intra-day RSDs <3.8% and inter-day RSDs <2.7%), and accurate (109.6-92.5%). This is the first report on the isolation and quantification of 1, 2, 4 and 12 in Z. armatum and 3 in Zanthoxylum genus. The methods: were successfully applied to assess the quality of samples collected from different locations of Himachal Pradesh during summer and winter season. The results demonstrated that flavonoids and furofuran lignans were the major constituents in Z. armatum leaves. The developed methods: were further applied for tandem electrospray ionization-mass spectrometry (UPLC-DAD-ESI-MS/MS) and total eighteen compounds were identified including phenolic acid, flavonoids, furofuran lignans, coumarin and isobutyl amides.

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Authors+Show Affiliations

Bhatt V
Department of Natural Product Chemistry and Process Development, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India; Academy of Scientific and Innovative Research, Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India.
Sharma S
Department of Natural Product Chemistry and Process Development, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India; Academy of Scientific and Innovative Research, Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India.
Kumar N
Department of Natural Product Chemistry and Process Development, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India; Academy of Scientific and Innovative Research, Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India.
Sharma U
Department of Natural Product Chemistry and Process Development, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India; Academy of Scientific and Innovative Research, Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India.
Singh B
Department of Natural Product Chemistry and Process Development, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India; Academy of Scientific and Innovative Research, Anusandhan Bhawan, 2 Rafi Marg, New Delhi, 110001, India. Electronic address: bikramsingh@ihbt.res.in.

MeSH

AmidesApigeninBenzodioxolesCatechinChromatography, High Pressure LiquidCoumarinsDioxolesFlavonoidsFuransHesperidinHydroxybenzoatesLignansLimit of DetectionPlant LeavesPowdersReproducibility of ResultsSeasonsSpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryZanthoxylum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27693952

Citation

Bhatt, Vinod, et al. "Simultaneous Quantification and Identification of Flavonoids, Lignans, Coumarin and Amides in Leaves of Zanthoxylum Armatum Using UPLC-DAD-ESI-QTOF-MS/MS." Journal of Pharmaceutical and Biomedical Analysis, vol. 132, 2017, pp. 46-55.
Bhatt V, Sharma S, Kumar N, et al. Simultaneous quantification and identification of flavonoids, lignans, coumarin and amides in leaves of Zanthoxylum armatum using UPLC-DAD-ESI-QTOF-MS/MS. J Pharm Biomed Anal. 2017;132:46-55.
Bhatt, V., Sharma, S., Kumar, N., Sharma, U., & Singh, B. (2017). Simultaneous quantification and identification of flavonoids, lignans, coumarin and amides in leaves of Zanthoxylum armatum using UPLC-DAD-ESI-QTOF-MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 132, 46-55. https://doi.org/10.1016/j.jpba.2016.09.035
Bhatt V, et al. Simultaneous Quantification and Identification of Flavonoids, Lignans, Coumarin and Amides in Leaves of Zanthoxylum Armatum Using UPLC-DAD-ESI-QTOF-MS/MS. J Pharm Biomed Anal. 2017 Jan 5;132:46-55. PubMed PMID: 27693952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantification and identification of flavonoids, lignans, coumarin and amides in leaves of Zanthoxylum armatum using UPLC-DAD-ESI-QTOF-MS/MS. AU - Bhatt,Vinod, AU - Sharma,Sushila, AU - Kumar,Neeraj, AU - Sharma,Upendra, AU - Singh,Bikram, Y1 - 2016/09/26/ PY - 2016/06/30/received PY - 2016/09/19/revised PY - 2016/09/24/accepted PY - 2016/10/4/pubmed PY - 2017/7/28/medline PY - 2016/10/4/entrez KW - Coumarin KW - Flavonoids KW - Furofuran lignans KW - Isobutyl amides KW - UPLC-DAD-ESI-QTOF-MS/MS KW - Zanthoxylum armatum leaves SP - 46 EP - 55 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 132 N2 - The current study presents isolation and characterization of twelve compounds including catechin (1), isovitexin (2), hesperidin (3), psoralin (4), eudesmin (5), kobusin (6), fargesin (7), sesamin (8), asarinin (9), planispine-A (10), α-sanshool (11) and vitexin (12), from the leaves of Zanthoxylum armatum. Further, two rapid and simple ultra performance liquid chromatography-diode array detection (UPLC-DAD) methods were developed for the simultaneous quantitative determination of isolated compounds from Z. armatum leaves. These analytical methods were validated for linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The LOD and LOQ were in the range of 0.06-0.21μg/mL and 0.19-0.69μg/mL, respectively. The validated method was linear (R2≥0.9906), precise in terms of peak area (intra-day RSDs <3.8% and inter-day RSDs <2.7%), and accurate (109.6-92.5%). This is the first report on the isolation and quantification of 1, 2, 4 and 12 in Z. armatum and 3 in Zanthoxylum genus. The methods: were successfully applied to assess the quality of samples collected from different locations of Himachal Pradesh during summer and winter season. The results demonstrated that flavonoids and furofuran lignans were the major constituents in Z. armatum leaves. The developed methods: were further applied for tandem electrospray ionization-mass spectrometry (UPLC-DAD-ESI-MS/MS) and total eighteen compounds were identified including phenolic acid, flavonoids, furofuran lignans, coumarin and isobutyl amides. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/27693952/Simultaneous_quantification_and_identification_of_flavonoids_lignans_coumarin_and_amides_in_leaves_of_Zanthoxylum_armatum_using_UPLC_DAD_ESI_QTOF_MS/MS_ DB - PRIME DP - Unbound Medicine ER -
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