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In vitro Antioxidant Activities and Polyphenol Contents of Seven Commercially Available Fruits.
Pharmacognosy Res 2016 Oct-Dec; 8(4):258-264PR

Abstract

BACKGROUND

Fruits are considered one of the richest sources of natural antioxidants. Their consumption has been linked to the prevention of oxidative stress-induced diseases.

OBJECTIVE

In this study, in vitro antioxidant activities of blueberry, jackfruit, blackberry, black raspberry, red raspberry, strawberry, and California table grape extracts were evaluated.

MATERIALS AND METHODS

Antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant potential (FRAP), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), nitric oxide (NO), superoxide anion (O2-) scavenging assays, and ferric reducing power.

RESULTS

Black raspberry extract had the highest phenolic (965.6 ± 2.9 mg gallic acid equivalents [GAE]/g), flavonoid (186.4 ± 1.7 mg quercetin equivalents/g), and proanthocyanidin (2677 ± 71.1 mg GAE/g) contents. All fruit extracts exhibited increasing radical scavenging activities with increased concentrations. At 100 μg/ml, red raspberry extract showed the highest ferric reducing power (A700 =0.3 ± 0.0052) and FRAP activity (A593 =11.43 mM Fe2+/g). Black raspberry extract (100 μg/ml) exhibited the highest DPPH activity (A517 =89.03 ± 0.0471). Jackfruit extract (100 μg/ml) had the highest ABTS (A734 =35.6 ± 0.613), NO (A540 =81.7 ± 0.2), and O2- radical scavenging (A230 =55.5 ± 0.2) activities. Positive correlations were observed between IC50 values for different radical scavenging activities and different polyphenolics. Red raspberry extract had the highest Pearson's coefficient values (0.952-1) between total phenolics, flavonoids, and proanthocyanidins and DPPH and superoxide radical scavenging activities.

CONCLUSIONS

The antioxidant rich fruits in this study are good source of functional food and nutraceuticals that have the potential to improve human health.

SUMMARY

All fruit extracts exhibited increasing radical scavenging activities with increased concentrationsBlack raspberry extract is enriched in total phenols, flavonoids, and proanthocyanidins and showed the highest 2,2-diphenyl-1-picrylhydrazyl scavenging activity and red raspberry extract showed the highest ferric reducing power and ferric reducing antioxidant potential activityJackfruit extract exhibited the highest 2,2'azino-bis (3-ethylbenzothiazoline-6-sulfonicacid) diammonium salt, nitric oxide, O2- scavenging activitiesPositive correlations were observed between IC50 values for different radical scavenging activities and different polyphenolics. Abbreviations Used: Abs: Absorbance, ABTS: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, BHT: Butylated hydroxytoluene, DPPH: 2,2-diphenyl-1-picrylhydrazyl, DW: Dry weight, FRAP: Ferric reducing antioxidant potential, FW: Fresh weight, GAE: Gallic acid equivalents, NADH: β-nicotinamide adenine dinucleotide hydrate, NFL: The National Food Laboratories, NO: Nitric oxide, ONPG: ortho-nitrophenyl-β-galactoside, PBS: Phosphate buffered saline, PMS: Phenazine methosulfate, QE: Quercetin equivalents, ROS: Reactive oxygen species, SD: Standard deviation, SOD: Superoxide dismutase, TCA: Trichloroacetic acid, TPTZ: 2,4,6-tris(2-pyridyl)-s-triazine, Trolox: (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid.

Authors+Show Affiliations

Department of Biology, Texas Woman's University, Denton, TX 76204-5799, USA.Department of Biology, Texas Woman's University, Denton, TX 76204-5799, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27695265

Citation

Basu, Paramita, and Camelia Maier. "In Vitro Antioxidant Activities and Polyphenol Contents of Seven Commercially Available Fruits." Pharmacognosy Research, vol. 8, no. 4, 2016, pp. 258-264.
Basu P, Maier C. In vitro Antioxidant Activities and Polyphenol Contents of Seven Commercially Available Fruits. Pharmacognosy Res. 2016;8(4):258-264.
Basu, P., & Maier, C. (2016). In vitro Antioxidant Activities and Polyphenol Contents of Seven Commercially Available Fruits. Pharmacognosy Research, 8(4), pp. 258-264.
Basu P, Maier C. In Vitro Antioxidant Activities and Polyphenol Contents of Seven Commercially Available Fruits. Pharmacognosy Res. 2016;8(4):258-264. PubMed PMID: 27695265.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro Antioxidant Activities and Polyphenol Contents of Seven Commercially Available Fruits. AU - Basu,Paramita, AU - Maier,Camelia, PY - 2016/10/4/entrez PY - 2016/10/4/pubmed PY - 2016/10/4/medline KW - Antioxidant activity KW - IC50 KW - flavonoids KW - phenols KW - proanthocyanidins KW - radical scavenging SP - 258 EP - 264 JF - Pharmacognosy research JO - Pharmacognosy Res VL - 8 IS - 4 N2 - BACKGROUND: Fruits are considered one of the richest sources of natural antioxidants. Their consumption has been linked to the prevention of oxidative stress-induced diseases. OBJECTIVE: In this study, in vitro antioxidant activities of blueberry, jackfruit, blackberry, black raspberry, red raspberry, strawberry, and California table grape extracts were evaluated. MATERIALS AND METHODS: Antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant potential (FRAP), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), nitric oxide (NO), superoxide anion (O2-) scavenging assays, and ferric reducing power. RESULTS: Black raspberry extract had the highest phenolic (965.6 ± 2.9 mg gallic acid equivalents [GAE]/g), flavonoid (186.4 ± 1.7 mg quercetin equivalents/g), and proanthocyanidin (2677 ± 71.1 mg GAE/g) contents. All fruit extracts exhibited increasing radical scavenging activities with increased concentrations. At 100 μg/ml, red raspberry extract showed the highest ferric reducing power (A700 =0.3 ± 0.0052) and FRAP activity (A593 =11.43 mM Fe2+/g). Black raspberry extract (100 μg/ml) exhibited the highest DPPH activity (A517 =89.03 ± 0.0471). Jackfruit extract (100 μg/ml) had the highest ABTS (A734 =35.6 ± 0.613), NO (A540 =81.7 ± 0.2), and O2- radical scavenging (A230 =55.5 ± 0.2) activities. Positive correlations were observed between IC50 values for different radical scavenging activities and different polyphenolics. Red raspberry extract had the highest Pearson's coefficient values (0.952-1) between total phenolics, flavonoids, and proanthocyanidins and DPPH and superoxide radical scavenging activities. CONCLUSIONS: The antioxidant rich fruits in this study are good source of functional food and nutraceuticals that have the potential to improve human health. SUMMARY: All fruit extracts exhibited increasing radical scavenging activities with increased concentrationsBlack raspberry extract is enriched in total phenols, flavonoids, and proanthocyanidins and showed the highest 2,2-diphenyl-1-picrylhydrazyl scavenging activity and red raspberry extract showed the highest ferric reducing power and ferric reducing antioxidant potential activityJackfruit extract exhibited the highest 2,2'azino-bis (3-ethylbenzothiazoline-6-sulfonicacid) diammonium salt, nitric oxide, O2- scavenging activitiesPositive correlations were observed between IC50 values for different radical scavenging activities and different polyphenolics. Abbreviations Used: Abs: Absorbance, ABTS: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, BHT: Butylated hydroxytoluene, DPPH: 2,2-diphenyl-1-picrylhydrazyl, DW: Dry weight, FRAP: Ferric reducing antioxidant potential, FW: Fresh weight, GAE: Gallic acid equivalents, NADH: β-nicotinamide adenine dinucleotide hydrate, NFL: The National Food Laboratories, NO: Nitric oxide, ONPG: ortho-nitrophenyl-β-galactoside, PBS: Phosphate buffered saline, PMS: Phenazine methosulfate, QE: Quercetin equivalents, ROS: Reactive oxygen species, SD: Standard deviation, SOD: Superoxide dismutase, TCA: Trichloroacetic acid, TPTZ: 2,4,6-tris(2-pyridyl)-s-triazine, Trolox: (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid. SN - 0976-4836 UR - https://www.unboundmedicine.com/medline/citation/27695265/In_vitro_Antioxidant_Activities_and_Polyphenol_Contents_of_Seven_Commercially_Available_Fruits_ L2 - http://www.phcogres.com/article.asp?issn=0974-8490;year=2016;volume=8;issue=4;spage=258;epage=264;aulast=Basu DB - PRIME DP - Unbound Medicine ER -