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Simultaneous determination of phenolic acids and flavonoids in Chenopodium formosanum Koidz. (djulis) by HPLC-DAD-ESI-MS/MS.
J Pharm Biomed Anal. 2017 Jan 05; 132:109-116.JP

Abstract

A high performance liquid chromatography-diode array detection-tandem mass spectrometry method (HPLC-DAD-MS/MS) was developed for simultaneous determination of phenolic acids and flavonoids in djulis (Chenopodium formosanum Koidz.), a traditional Chinese herb reported to possess vital biological activities. A high yield of phenolic acids and flavonoids was attained by employing 50% ethanol in water as the extraction solvent and shaking in a 60°C water bath for 3h. A total of 8 phenolic acids and 14 flavonoids were separated and identified within 55min by using a Poroshell 120 EC-C18 column with detection at 280nm, flow rate at 0.8mL/min, column temperature at 35°C, and a gradient solvent system of 0.1% formic acid in water and acetonitrile. Two internal standards caffeic acid and kaempferol-3-O-rutinoside were used for quantitation of phenolic acids and flavonoids in djulis respectively. The amounts of phenolic acids ranged from 11.5±0.8μg/g (caffeoyl-putrescine-derivative (2)) to 1855.3±16.9μg/g (hydroxylphenylacetic acid pentoside), while the flavonoids ranged from 19.93±2.29μg/g (quercetin-3-O-(coumaryl)-rutinoside-pentoside (1)) to 257.3±2.05μg/g (rutin-O-pentoside (2)). A high recovery (89.68-97.20%) and high reproducibility was obtained for both phenolic acids and flavonoids with the relative standard deviation (RSD) for the latter ranging from 0.09-8.22% (intra-day variability) and 0.80-8.48% (inter-day variability). This method may be applied to determination of both phenolic acids and flavonoids in food products and Chinese herbs.

Authors+Show Affiliations

Department of Food and Beverage Management, Lee-Ming Institute of Technology, New Taipei City 242, Taiwan.Department of Food Science, Fu Jen Catholic University, New Taipei City 242, Taiwan.Department of Food Science, Fu Jen Catholic University, New Taipei City 242, Taiwan.Department of Food Science, Fu Jen Catholic University, New Taipei City 242, Taiwan. Electronic address: 002622@mail.fju.edu.tw.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27701037

Citation

Hsu, B Y., et al. "Simultaneous Determination of Phenolic Acids and Flavonoids in Chenopodium Formosanum Koidz. (djulis) By HPLC-DAD-ESI-MS/MS." Journal of Pharmaceutical and Biomedical Analysis, vol. 132, 2017, pp. 109-116.
Hsu BY, Lin SW, Inbaraj BS, et al. Simultaneous determination of phenolic acids and flavonoids in Chenopodium formosanum Koidz. (djulis) by HPLC-DAD-ESI-MS/MS. J Pharm Biomed Anal. 2017;132:109-116.
Hsu, B. Y., Lin, S. W., Inbaraj, B. S., & Chen, B. H. (2017). Simultaneous determination of phenolic acids and flavonoids in Chenopodium formosanum Koidz. (djulis) by HPLC-DAD-ESI-MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 132, 109-116. https://doi.org/10.1016/j.jpba.2016.09.027
Hsu BY, et al. Simultaneous Determination of Phenolic Acids and Flavonoids in Chenopodium Formosanum Koidz. (djulis) By HPLC-DAD-ESI-MS/MS. J Pharm Biomed Anal. 2017 Jan 5;132:109-116. PubMed PMID: 27701037.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of phenolic acids and flavonoids in Chenopodium formosanum Koidz. (djulis) by HPLC-DAD-ESI-MS/MS. AU - Hsu,B Y, AU - Lin,S W, AU - Inbaraj,B Stephen, AU - Chen,B H, Y1 - 2016/09/26/ PY - 2016/06/07/received PY - 2016/09/12/revised PY - 2016/09/24/accepted PY - 2016/10/5/pubmed PY - 2017/7/28/medline PY - 2016/10/5/entrez KW - Chenopodium formosanum koidz. (Djulis) KW - Flavonoids KW - HPLC-DAD-MS/MS KW - Phenolic acids SP - 109 EP - 116 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 132 N2 - A high performance liquid chromatography-diode array detection-tandem mass spectrometry method (HPLC-DAD-MS/MS) was developed for simultaneous determination of phenolic acids and flavonoids in djulis (Chenopodium formosanum Koidz.), a traditional Chinese herb reported to possess vital biological activities. A high yield of phenolic acids and flavonoids was attained by employing 50% ethanol in water as the extraction solvent and shaking in a 60°C water bath for 3h. A total of 8 phenolic acids and 14 flavonoids were separated and identified within 55min by using a Poroshell 120 EC-C18 column with detection at 280nm, flow rate at 0.8mL/min, column temperature at 35°C, and a gradient solvent system of 0.1% formic acid in water and acetonitrile. Two internal standards caffeic acid and kaempferol-3-O-rutinoside were used for quantitation of phenolic acids and flavonoids in djulis respectively. The amounts of phenolic acids ranged from 11.5±0.8μg/g (caffeoyl-putrescine-derivative (2)) to 1855.3±16.9μg/g (hydroxylphenylacetic acid pentoside), while the flavonoids ranged from 19.93±2.29μg/g (quercetin-3-O-(coumaryl)-rutinoside-pentoside (1)) to 257.3±2.05μg/g (rutin-O-pentoside (2)). A high recovery (89.68-97.20%) and high reproducibility was obtained for both phenolic acids and flavonoids with the relative standard deviation (RSD) for the latter ranging from 0.09-8.22% (intra-day variability) and 0.80-8.48% (inter-day variability). This method may be applied to determination of both phenolic acids and flavonoids in food products and Chinese herbs. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/27701037/Simultaneous_determination_of_phenolic_acids_and_flavonoids_in_Chenopodium_formosanum_Koidz___djulis__by_HPLC_DAD_ESI_MS/MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(16)30699-9 DB - PRIME DP - Unbound Medicine ER -